##################################
# NO CUT ENZYMES ( 38 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AvrII BciVI
BlnI BspDI BspLU11I Bst1107I BstZ17I
ClaI CpoI EcoRI EcoRV EcoT22I
HpaI KpnI MluI NdeI NheI
NotI NsiI PacI PinAI PshBI
Psp1406I PvuI RsrII SalI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 781 GTCTAC GT^[AC][GT]AC
AccIII 2207 TCCGGA T^CCGGA
AflII 502 CTTAAG C^TTAAG
Asp700I 282 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3848 TGCGCA TGC^GCA
BbeI 4567 GGCGCC GGCGC^C
BclI 1469 TGATCA T^GATCA
BfaI 1240 CTAG C^TAG
BfrI 502 CTTAAG C^TTAAG
BsaBI 3711 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2207 TCCGGA [AT]^CCGG[AT]
BseAI 2207 TCCGGA T^CCGGA
BsiEI 1921 CGGCCG CG[GA][CT]^CG
BsiWI 2072 CGTACG C^GTACG
BspEI 2207 TCCGGA T^CCGGA
BssHII 390 GCGCGC G^CGCGC
BstBI 3619 TTCGAA TT^CGAA
DrdI 1497 GACCTACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1921 CGGCCG C^GGCCG
EclXI 1921 CGGCCG C^GGCCG
Eco52I 1921 CGGCCG C^GGCCG
FbaI 1469 TGATCA T^GATCA
FseI 5067 GGCCGGCC GGCCGG^CC
FspI 3848 TGCGCA TGC^GCA
HincII 1276 GTCAAC GT[CT]^[GA]AC
HindII 1276 GTCAAC GT[CT]^[GA]AC
HindIII 3535 AAGCTT A^AGCTT
KasI 4567 GGCGCC G^GCGCC
MaeI 1240 CTAG C^TAG
MamI 3711 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2796 CAATTG C^AATTG
MroI 2207 TCCGGA T^CCGGA
MunI 2796 CAATTG C^AATTG
NarI 4567 GGCGCC GG^CGCC
NruI 3384 TCGCGA TCG^CGA
NspV 3619 TTCGAA TT^CGAA
PaeR7I 2545 CTCGAG C^TCGAG
PmeI 3663 GTTTAAAC GTTT^AAAC
PshAI 6160 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1658 AGTACT AGT^ACT
SfoI 4567 GGCGCC GGC^GCC
SfuI 3619 TTCGAA TT^CGAA
SgrAI 4844 CGCCGGTG C[GA]^CCGG[CT]G
SspI 5752 AATATT AAT^ATT
XhoI 2545 CTCGAG C^TCGAG
XmnI 282 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 295 AAATTC [GA]^AATT[CT]
6449 AAATTC
AflIII 5283 ACACGT A^C[GA][CT]GT
5285 ACGTGT
AhdI 1411 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3051 GACCCTTGGTC
Aor51HI 4281 AGCGCT AGC^GCT
4931 AGCGCT
ApaI 4755 GGGCCC GGGCC^C
6389 GGGCCC
ApoI 295 AAATTC [GA]^AATT[CT]
6449 AAATTC
AspEI 1411 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3051 GACCCTTGGTC
BamHI 3512 GGATCC G^GATCC
5416 GGATCC
BbrPI 1621 CACGTG CAC^GTG
5284 CACGTG
BlpI 1188 GCTGAGC GC^T[ACGT]AGC
2382 GCTGAGC
Bpu1102I 1188 GCTGAGC GC^T[ACGT]AGC
2382 GCTGAGC
BsmBI 3854 GAGACG CGTCTC(1/5)
5384 GAGACG
Bsp1407I 3682 TGTACA T^GTACA
6430 TGTACA
BsrDI 935 GCAATG GCAATG(2/0)
1217 GCAATG
BsrGI 3682 TGTACA T^GTACA
6430 TGTACA
BstAPI 1617 GCACCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5135 GCAGCTGGTGC
BstXI 930 CCATAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2049 CCAGATTCGTGG
CelII 1188 GCTGAGC GC^T[ACGT]AGC
2382 GCTGAGC
DraI 3664 TTTAAA TTT^AAA
6423 TTTAAA
Eam1105I 1411 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3051 GACCCTTGGTC
Eco47III 4281 AGCGCT AGC^GCT
4931 AGCGCT
PmaCI 1621 CACGTG CAC^GTG
5284 CACGTG
PmlI 1621 CACGTG CAC^GTG
5284 CACGTG
SacI 4507 GAGCTC GAGCT^C
4594 GAGCTC
SbfI 564 CCTGCAGG CCTGCA^GG
1869 CCTGCAGG
SexAI 1907 ACCTGGT A^CC[AT]GGT
3566 ACCAGGT
SfiI 1992 GGCCACGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3873 GGCCCTGGTGGCC
SmaI 5 CCCGGG CCC^GGG
1516 CCCGGG
SphI 410 GCATGC GCATG^C
4643 GCATGC
Sse8387I 564 CCTGCAGG CCTGCA^GG
1869 CCTGCAGG
SspBI 3682 TGTACA T^GTACA
6430 TGTACA
XmaCI 5 CCCGGG C^CCGGG
1516 CCCGGG
XmaI 5 CCCGGG C^CCGGG
1516 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII