##################################
# NO CUT ENZYMES ( 34 enzymes) #
##################################
Acc65I AclI AflII AgeI Asp718I
BfrI BsiWI EagI EclXI Eco52I
FseI HpaI KpnI KspI MfeI
MluI MunI NdeI NotI NruI
PacI PinAI PmeI PshAI Psp1406I
PvuI SacII SbfI SfiI SgrAI
SnaBI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 414 GTGCAC G^TGCAC
ApaLI 414 GTGCAC G^TGCAC
AscI 7342 GGCGCGCC GG^CGCGCC
AviII 7111 TGCGCA TGC^GCA
AvrII 4073 CCTAGG C^CTAGG
BcgI 7808 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4073 CCTAGG C^CTAGG
BsaBI 8359 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 304 CGGTCG CG[GA][CT]^CG
BspDI 7239 ATCGAT AT^CGAT
Bst1107I 4844 GTATAC GTA^TAC
BstBI 1668 TTCGAA TT^CGAA
BstZ17I 4844 GTATAC GTA^TAC
ClaI 7239 ATCGAT AT^CGAT
EcoRV 5952 GATATC GAT^ATC
FspI 7111 TGCGCA TGC^GCA
HindIII 5716 AAGCTT A^AGCTT
MamI 8359 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 1668 TTCGAA TT^CGAA
SalI 6399 GTCGAC G^TCGAC
ScaI 4773 AGTACT AGT^ACT
SfuI 1668 TTCGAA TT^CGAA
SpeI 9656 ACTAGT A^CTAGT
SspI 9982 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2886 GACGTC GACGT^C
7200 GACGTC
AflIII 6187 ACATGT A^C[GA][CT]GT
8784 ACATGT
Aor51HI 3625 AGCGCT AGC^GCT
6616 AGCGCT
AseI 5502 ATTAAT AT^TAAT
8360 ATTAAT
AsnI 5502 ATTAAT AT^TAAT
8360 ATTAAT
BamHI 5199 GGATCC G^GATCC
7358 GGATCC
BbeI 250 GGCGCC GGCGC^C
272 GGCGCC
BbrPI 438 CACGTG CAC^GTG
6885 CACGTG
BclI 3763 TGATCA T^GATCA
9490 TGATCA
BsaAI 438 CACGTG [CT]AC^GT[GA]
6885 CACGTG
Bsp1407I 2389 TGTACA T^GTACA
10685 TGTACA
BspHI 10876 TCATGA T^CATGA
10968 TCATGA
BspLU11I 6187 ACATGT A^CATGT
8784 ACATGT
BsrGI 2389 TGTACA T^GTACA
10685 TGTACA
BssHII 185 GCGCGC G^CGCGC
7343 GCGCGC
CpoI 2985 CGGACCG CG^G[AT]CCG
7843 CGGACCG
Eco47III 3625 AGCGCT AGC^GCT
6616 AGCGCT
EcoT22I 5879 ATGCAT ATGCA^T
8570 ATGCAT
FbaI 3763 TGATCA T^GATCA
9490 TGATCA
KasI 250 GGCGCC G^GCGCC
272 GGCGCC
NarI 250 GGCGCC GG^CGCC
272 GGCGCC
NheI 4460 GCTAGC G^CTAGC
4752 GCTAGC
NsiI 5879 ATGCAT ATGCA^T
8570 ATGCAT
PaeR7I 2779 CTCGAG C^TCGAG
7253 CTCGAG
PmaCI 438 CACGTG CAC^GTG
6885 CACGTG
PmlI 438 CACGTG CAC^GTG
6885 CACGTG
PshBI 5502 ATTAAT AT^TAAT
8360 ATTAAT
RcaI 10876 TCATGA T^CATGA
10968 TCATGA
RsrII 2985 CGGACCG CG^G[AT]CCG
7843 CGGACCG
SexAI 4088 ACCAGGT A^CC[AT]GGT
8772 ACCTGGT
SfoI 250 GGCGCC GGC^GCC
272 GGCGCC
SphI 1702 GCATGC GCATG^C
2074 GCATGC
SspBI 2389 TGTACA T^GTACA
10685 TGTACA
XhoI 2779 CTCGAG C^TCGAG
7253 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AhdI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI