HUGE

Gene/Protein Characteristic Table for KIAA1422

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB037843
Description :	Potassium channel subfamily T member 1.
HUGO Gene Name :	potassium channel, subfamily T, member 1 (KCNT1)
Clone Name :	hk10484 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 4284 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 0 bp

Genome contig ID gi89161216f_137633859
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AAGGCGCCCAAGCAGGCAGGCCGGGCGGCGGCCGC
Flanking genome sequence
(184218 - 184267)
----+----*----+----*----+----*----+----*----+----*
GGAGTGGATCAGCCAGCAGCGCCTCAGCCTGTACCGGCGCTCTGAGCGCC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 9 f 137733859 137818075 29 99.8 Perfect prediction

Features of the protein sequence	Description

Length: 1151 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW88178	100.0	potassium chann...	Homo sapiens
NP_065873	100.0	potassium chann...	Homo sapiens
AAI71770	99.9	Unknown (protei...	Homo sapiens
EAW88177	99.6	potassium chann...	Homo sapiens
XP_001118025	93.1	similar to pota...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR013099 299 377 PF07885 Ion transport 2

IPR003929 516 637 PF03493 Calcium-activated BK potassium channel

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013099	299	377	PF07885	Ion transport 2
IPR003929	516	637	PF03493	Calcium-activated BK potassium channel

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 145 KLLTCLLYIVRVLLDDPALGIGC 167 SECONDARY 23

2 199 WAIQVIVAIISFLETMLLIYLSY 221 PRIMARY 23

3 229 IFRVSFVLEMINTLPFIITIFWP 251 PRIMARY 23

4 288 AMFNQVLILFCTLLCLVFTGTCG 310 SECONDARY 23

5 317 AGENLSLLTSFYFCIVTFSTVGY 339 SECONDARY 23

6 347 WPSQLLVVIMICVALVVLPLQFE 369 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	145	KLLTCLLYIVRVLLDDPALGIGC	167	SECONDARY	23
2	199	WAIQVIVAIISFLETMLLIYLSY	221	PRIMARY	23
3	229	IFRVSFVLEMINTLPFIITIFWP	251	PRIMARY	23
4	288	AMFNQVLILFCTLLCLVFTGTCG	310	SECONDARY	23
5	317	AGENLSLLTSFYFCIVTFSTVGY	339	SECONDARY	23
6	347	WPSQLLVVIMICVALVVLPLQFE	369	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TACAACTTCATCGTGCCACTG

Primer_r
: ACCAGGTTGTCCGCATAGATG

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 9

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTCAGAGCCCAGGTAAGCAAC
Primer_r	: CAGTGCAGGAGGGAGTGAGTG
PCR product length	: 210 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	0 bp
Genome contig ID	gi89161216f_137633859
PolyA signal sequence (None)	+--------+--------+----*----+---- AAGGCGCCCAAGCAGGCAGGCCGGGCGGCGGCCGC
Flanking genome sequence (184218 - 184267)	----+--------+--------+--------+--------+----* GGAGTGGATCAGCCAGCAGCGCCTCAGCCTGTACCGGCGCTCTGAGCGCC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	9	f	137733859	137818075	29	99.8	Perfect prediction

Primer_f	: TACAACTTCATCGTGCCACTG
Primer_r	: ACCAGGTTGTCCGCATAGATG
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1422

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information