##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AccII AccIII AclI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AviII BbeI BbrPI BbvCI BcgI
BseAI BsiWI BspDI BspEI BssHII
BstUI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NsiI PacI PflMI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SfiI SfoI SgrAI SmaI
SphI SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AgeI 5467 ACCGGT A^CCGGT
AvrII 1590 CCTAGG C^CTAGG
BamHI 6503 GGATCC G^GATCC
BanI 6202 GGTGCC G^G[CT][GA]CC
BclI 2405 TGATCA T^GATCA
BcnI 2254 CCGGG CC^[GC]GG
BlnI 1590 CCTAGG C^CTAGG
BsaWI 5467 ACCGGT [AT]^CCGG[AT]
BsiEI 5614 CGACCG CG[GA][CT]^CG
BsmBI 1405 CGTCTC CGTCTC(1/5)
BspLU11I 5296 ACATGT A^CATGT
BsrBI 5432 GAGCGG CCGCTC(-3/-3)
BsrFI 5467 ACCGGT [GA]^CCGG[CT]
BssSI 5599 CACGAG CACGAG(-5/-1)
Bst1107I 6047 GTATAC GTA^TAC
BstBI 3880 TTCGAA TT^CGAA
BstZ17I 6047 GTATAC GTA^TAC
CfoI 5357 GCGC GCG^C
Cfr10I 5467 ACCGGT [GA]^CCGG[CT]
DraIII 5316 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3014 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2405 TGATCA T^GATCA
HhaI 5357 GCGC GCG^C
HinP1I 5357 GCGC G^CGC
HpaI 5147 GTTAAC GTT^AAC
NciI 2254 CCGGG CC^[GC]GG
NheI 3044 GCTAGC G^CTAGC
NspV 3880 TTCGAA TT^CGAA
PaeR7I 2333 CTCGAG C^TCGAG
PinAI 5467 ACCGGT A^CCGGT
SalI 4765 GTCGAC G^TCGAC
SbfI 957 CCTGCAGG CCTGCA^GG
ScaI 665 AGTACT AGT^ACT
SfuI 3880 TTCGAA TT^CGAA
SnaBI 6415 TACGTA TAC^GTA
SpeI 4119 ACTAGT A^CTAGT
Sse8387I 957 CCTGCAGG CCTGCA^GG
SspI 5901 AATATT AAT^ATT
XbaI 3716 TCTAGA T^CTAGA
XhoI 2333 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1273 GACGTC GACGT^C
1403 GACGTC
AflII 996 CTTAAG C^TTAAG
3780 CTTAAG
AflIII 5296 ACATGT A^C[GA][CT]GT
5880 ACACGT
AhdI 2709 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2782 GACCCCTTGTC
AlwNI 175 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
4585 CAGGCTCTG
AocI 2168 CCTAAGG CC^T[ACGT]AGG
4776 CCTGAGG
AseI 2510 ATTAAT AT^TAAT
3446 ATTAAT
AsnI 2510 ATTAAT AT^TAAT
3446 ATTAAT
AspEI 2709 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2782 GACCCCTTGTC
AspHI 1396 GAGCAC G[AT]GC[AT]^C
4448 GAGCAC
AvaI 1783 CTCGGG C^[CT]CG[GA]G
2333 CTCGAG
BciVI 257 GGATAC GTATCC(6/5)
6302 GGATAC
BfrI 996 CTTAAG C^TTAAG
3780 CTTAAG
BglI 2207 GCCTTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4626 GCCTCCCTGGC
BglII 1044 AGATCT A^GATCT
1724 AGATCT
BsaAI 2655 CACGTA [CT]AC^GT[GA]
6415 TACGTA
BsaBI 2593 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2945 GATAGACATC
BsgI 4011 GTGCAG GTGCAG(16/14)
6129 GTGCAG
BsiHKAI 1396 GAGCAC G[AT]GC[AT]^C
4448 GAGCAC
BsoBI 1783 CTCGGG C^[CT]CG[GA]G
2333 CTCGAG
Bsp1407I 315 TGTACA T^GTACA
2875 TGTACA
BspMI 293 ACCTGC ACCTGC(4/8)
2268 ACCTGC
BsrGI 315 TGTACA T^GTACA
2875 TGTACA
BstAPI 3206 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5766 GCAGCGAATGC
BstXI 967 CCAGATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5659 CCAGCACCTTGG
Bsu36I 2168 CCTAAGG CC^T[ACGT]AGG
4776 CCTGAGG
DrdI 2897 GACGACTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3093 GACACTGGAGTC
Eam1105I 2709 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2782 GACCCCTTGTC
Eco81I 2168 CCTAAGG CC^T[ACGT]AGG
4776 CCTGAGG
EcoRV 4430 GATATC GAT^ATC
6269 GATATC
FauI 538 CCCGC CCCGC(4/6)
5434 GCGGG
HgaI 2191 GACGC GACGC(5/10)
3397 GCGTC
HindIII 520 AAGCTT A^AGCTT
5732 AAGCTT
MamI 2593 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2945 GATAGACATC
NcoI 598 CCATGG C^CATGG
2829 CCATGG
NdeI 2602 CATATG CA^TATG
3458 CATATG
PshAI 4082 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4758 GACTCCAGTC
PshBI 2510 ATTAAT AT^TAAT
3446 ATTAAT
PstI 958 CTGCAG CTGCA^G
5764 CTGCAG
SexAI 677 ACCTGGT A^CC[AT]GGT
1237 ACCAGGT
SspBI 315 TGTACA T^GTACA
2875 TGTACA
StuI 13 AGGCCT AGG^CCT
4624 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspI
AvaII BalI BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII Hin1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmnI