##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AgeI AscI AseI AsnI AvrII
BamHI BbeI BlnI BsiWI BspDI
BssHII BstBI ClaI CpoI DraIII
EcoRV FseI HpaI KasI MfeI
MluI MunI NarI NcoI NdeI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2604 GACGTC GACGT^C
AccIII 849 TCCGGA T^CCGGA
AclI 2103 AACGTT AA^CGTT
AhdI 3202 GACCACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 6679 AGCGCT AGC^GCT
AspEI 3202 GACCACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6424 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 151 TGCGCA TGC^GCA
BbrPI 711 CACGTG CAC^GTG
BclI 80 TGATCA T^GATCA
BglI 4649 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 849 TCCGGA T^CCGGA
Bsp1407I 603 TGTACA T^GTACA
BspEI 849 TCCGGA T^CCGGA
BspHI 1471 TCATGA T^CATGA
BspLU11I 4630 ACATGT A^CATGT
BsrGI 603 TGTACA T^GTACA
Bst1107I 5062 GTATAC GTA^TAC
BstZ17I 5062 GTATAC GTA^TAC
Eam1105I 3202 GACCACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6679 AGCGCT AGC^GCT
FbaI 80 TGATCA T^GATCA
FspI 151 TGCGCA TGC^GCA
HincII 1614 GTCAAC GT[CT]^[GA]AC
HindII 1614 GTCAAC GT[CT]^[GA]AC
MroI 849 TCCGGA T^CCGGA
NheI 5183 GCTAGC G^CTAGC
PflFI 6424 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 711 CACGTG CAC^GTG
PmlI 711 CACGTG CAC^GTG
Psp1406I 2103 AACGTT AA^CGTT
PvuI 2203 CGATCG CGAT^CG
RcaI 1471 TCATGA T^CATGA
SnaBI 5059 TACGTA TAC^GTA
SspBI 603 TGTACA T^GTACA
SspI 5618 AATATT AAT^ATT
Tth111I 6424 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 2848 GGTACC G^GTACC
5291 GGTACC
AflII 4944 CTTAAG C^TTAAG
6299 CTTAAG
Asp718I 2848 GGTACC G^GTACC
5291 GGTACC
BcgI 1894 CGACAACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6659 GCACACCCTTCG
BfrI 4944 CTTAAG C^TTAAG
6299 CTTAAG
BglII 4940 AGATCT A^GATCT
5111 AGATCT
BsaBI 2702 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3755 GATACCGATC
BsmI 185 GAATGC GAATGC(1/-1)
6878 GAATGC
BstEII 1709 GGTGACC G^GT[ACGT]ACC
4047 GGTGACC
BstPI 1709 GGTGACC G^GT[ACGT]ACC
4047 GGTGACC
DraI 6247 TTTAAA TTT^AAA
7001 TTTAAA
EcoNI 972 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1657 CCTCAGTAAGG
EcoO65I 1709 GGTGACC G^GT[ACGT]ACC
4047 GGTGACC
EcoRI 471 GAATTC G^AATTC
6164 GAATTC
KpnI 2848 GGTACC GGTAC^C
5291 GGTACC
MamI 2702 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3755 GATACCGATC
NaeI 1393 GCCGGC GCC^GGC
2277 GCCGGC
NgoMIV 1393 GCCGGC G^CCGGC
2277 GCCGGC
SphI 5759 GCATGC GCATG^C
6469 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI