##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AspEI AviII
BbeI BbrPI BcgI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I FseI
FspI Hin1I HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 5778 ACATGT A^C[GA][CT]GT
ApaI 5186 GGGCCC GGGCC^C
AspHI 246 GAGCTC G[AT]GC[AT]^C
AvrII 4893 CCTAGG C^CTAGG
BamHI 1285 GGATCC G^GATCC
BanI 3338 GGTGCC G^G[CT][GA]CC
BciVI 844 GGATAC GTATCC(6/5)
BglI 1418 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 363 AGATCT A^GATCT
BlnI 4893 CCTAGG C^CTAGG
BlpI 285 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 285 GCTTAGC GC^T[ACGT]AGC
BsiHKAI 246 GAGCTC G[AT]GC[AT]^C
BspLU11I 5778 ACATGT A^CATGT
Bst1107I 5774 GTATAC GTA^TAC
BstXI 5300 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5774 GTATAC GTA^TAC
CelII 285 GCTTAGC GC^T[ACGT]AGC
DraII 5185 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 5185 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2581 GAATTC G^AATTC
EcoRV 4520 GATATC GAT^ATC
HaeII 1742 AGCGCC [GA]GCGC^[CT]
HgaI 33 GACGC GACGC(5/10)
HincII 3314 GTTGAC GT[CT]^[GA]AC
HindII 3314 GTTGAC GT[CT]^[GA]AC
MaeII 1693 ACGT A^CGT
MfeI 5418 CAATTG C^AATTG
MspA1I 3657 CAGCTG C[AC]G^C[GT]G
MunI 5418 CAATTG C^AATTG
PvuII 3657 CAGCTG CAG^CTG
SacI 246 GAGCTC GAGCT^C
ScaI 3150 AGTACT AGT^ACT
SexAI 3334 ACCTGGT A^CC[AT]GGT
SmaI 1592 CCCGGG CCC^GGG
SpeI 5495 ACTAGT A^CTAGT
SphI 1881 GCATGC GCATG^C
StuI 957 AGGCCT AGG^CCT
XbaI 1326 TCTAGA T^CTAGA
XcmI 3737 CCATGTGCCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1592 CCCGGG C^CCGGG
XmaI 1592 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 426 CGCG CG^CG
5197 CGCG
AflII 1134 CTTAAG C^TTAAG
2769 CTTAAG
AocI 1289 CCTTAGG CC^T[ACGT]AGG
4820 CCTCAGG
AspI 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5063 GACAGAGTC
AvaI 1592 CCCGGG C^[CT]CG[GA]G
5165 CCCGAG
AvaII 3257 GGTCC G^G[AT]CC
5177 GGACC
BbsI 1006 GAAGAC GAAGAC(2/6)
2776 GTCTTC
BbvCI 5156 CCTCAGC CCTCAGC(-5/-2)
2381 GCTGAGG
BclI 414 TGATCA T^GATCA
5760 TGATCA
BfrI 1134 CTTAAG C^TTAAG
2769 CTTAAG
BpuAI 1006 GAAGAC GAAGAC(2/6)
2776 GTCTTC
BsaBI 1543 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4884 GATGTGCATC
BsaI 733 GAGACC GGTCTC(1/5)
3640 GGTCTC
BseRI 28 GAGGAG GAGGAG(10/8)
2220 GAGGAG
BsmBI 31 GAGACG CGTCTC(1/5)
5228 GAGACG
BsoBI 1592 CCCGGG C^[CT]CG[GA]G
5165 CCCGAG
BssSI 1163 CACGAG CACGAG(-5/-1)
1222 CACGAG
BstAPI 2744 GCACTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4109 GCAAGTGCTGC
BstUI 426 CGCG CG^CG
5197 CGCG
Bsu36I 1289 CCTTAGG CC^T[ACGT]AGG
4820 CCTCAGG
Eco81I 1289 CCTTAGG CC^T[ACGT]AGG
4820 CCTCAGG
FbaI 414 TGATCA T^GATCA
5760 TGATCA
MamI 1543 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4884 GATGTGCATC
MvnI 426 CGCG CG^CG
5197 CGCG
NcoI 965 CCATGG C^CATGG
4423 CCATGG
PflFI 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5063 GACAGAGTC
PstI 2855 CTGCAG CTGCA^G
4368 CTGCAG
Tth111I 54 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5063 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI