##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BbvCI
BcnI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspEI
BsrBI BsrFI BssHII BstBI BstUI
Cfr10I CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I FauI
FseI FspI HapII HgaI Hin1I
HpaII KasI KpnI KspI MluI
MroI MspI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AocI 818 CCTGAGG CC^T[ACGT]AGG
AspI 1517 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3065 GGATCC G^GATCC
BcgI 1759 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 946 GTATCC GTATCC(6/5)
BglI 2950 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 53 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 53 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 2336 TGTACA T^GTACA
BspDI 2750 ATCGAT AT^CGAT
BspLU11I 4031 ACATGT A^CATGT
BsrGI 2336 TGTACA T^GTACA
BssSI 5362 CACGAG CACGAG(-5/-1)
BstAPI 3495 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2236 CCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 818 CCTGAGG CC^T[ACGT]AGG
CelII 53 GCTGAGC GC^T[ACGT]AGC
ClaI 2750 ATCGAT AT^CGAT
Eco81I 818 CCTGAGG CC^T[ACGT]AGG
EcoNI 2831 CCTGTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 670 GAATTC G^AATTC
MspA1I 2424 CAGCTG C[AC]G^C[GT]G
NheI 3994 GCTAGC G^CTAGC
PaeR7I 5081 CTCGAG C^TCGAG
PflFI 1517 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 599 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 553 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 4030 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4299 CTGCAG CTGCA^G
PvuII 2424 CAGCTG CAG^CTG
SexAI 3662 ACCAGGT A^CC[AT]GGT
SspBI 2336 TGTACA T^GTACA
Tth111I 1517 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 599 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5197 TCTAGA T^CTAGA
XcmI 2493 CCAAAGAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5081 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 1490 CTTAAG C^TTAAG
4433 CTTAAG
AseI 3731 ATTAAT AT^TAAT
4292 ATTAAT
AsnI 3731 ATTAAT AT^TAAT
4292 ATTAAT
BfrI 1490 CTTAAG C^TTAAG
4433 CTTAAG
BsaAI 1475 CACGTA [CT]AC^GT[GA]
2796 TACGTG
BspHI 394 TCATGA T^CATGA
4900 TCATGA
BspMI 3500 ACCTGC ACCTGC(4/8)
4825 ACCTGC
Bst1107I 3646 GTATAC GTA^TAC
4890 GTATAC
BstZ17I 3646 GTATAC GTA^TAC
4890 GTATAC
DraII 553 AGGACCC [GA]G^G[ACGT]CC[CT]
5130 AGGCCCT
EcoO109I 553 AGGACCC [GA]G^G[ACGT]CC[CT]
5130 AGGCCCT
EcoRV 2016 GATATC GAT^ATC
3182 GATATC
HpaI 1147 GTTAAC GTT^AAC
3043 GTTAAC
PshBI 3731 ATTAAT AT^TAAT
4292 ATTAAT
RcaI 394 TCATGA T^CATGA
4900 TCATGA
SapI 3674 GCTCTTC GCTCTTC(1/4)
3897 GCTCTTC
ScaI 2484 AGTACT AGT^ACT
4495 AGTACT
SspI 693 AATATT AAT^ATT
5433 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI Eco47III EcoO65I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MunI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI