##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BbvCI
BcgI BglI BlnI BsaHI BsaWI
BseAI BsgI BsiWI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FauI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PpuMI PstI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Alw44I 2185 GTGCAC G^TGCAC
ApaLI 2185 GTGCAC G^TGCAC
AseI 3394 ATTAAT AT^TAAT
AsnI 3394 ATTAAT AT^TAAT
AvaI 1104 CCCGGG C^[CT]CG[GA]G
BamHI 3749 GGATCC G^GATCC
BanII 1966 GAGCTC G[GA]GC[CT]^C
BbrPI 324 CACGTG CAC^GTG
BciVI 3634 GGATAC GTATCC(6/5)
BglII 2197 AGATCT A^GATCT
BlpI 1968 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1968 GCTCAGC GC^T[ACGT]AGC
BsaAI 324 CACGTG [CT]AC^GT[GA]
BsaI 1488 GGTCTC GGTCTC(1/5)
BsiEI 1148 CGATCG CG[GA][CT]^CG
BsoBI 1104 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1696 TGTACA T^GTACA
BspDI 1146 ATCGAT AT^CGAT
BspMI 3234 ACCTGC ACCTGC(4/8)
BsrGI 1696 TGTACA T^GTACA
BstBI 143 TTCGAA TT^CGAA
CelII 1968 GCTCAGC GC^T[ACGT]AGC
ClaI 1146 ATCGAT AT^CGAT
EcoRI 343 GAATTC G^AATTC
EcoT14I 3268 CCAAGG C^C[AT][AT]GG
HapII 1105 CCGG C^CGG
HgaI 2128 GACGC GACGC(5/10)
HpaII 1105 CCGG C^CGG
MfeI 1686 CAATTG C^AATTG
MspA1I 1513 CAGCGG C[AC]G^C[GT]G
MspI 1105 CCGG C^CGG
MunI 1686 CAATTG C^AATTG
NspV 143 TTCGAA TT^CGAA
PmaCI 324 CACGTG CAC^GTG
PmlI 324 CACGTG CAC^GTG
PshAI 747 GACGTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3394 ATTAAT AT^TAAT
PvuI 1148 CGATCG CGAT^CG
SacI 1966 GAGCTC GAGCT^C
SapI 2427 GAAGAGC GCTCTTC(1/4)
ScaI 693 AGTACT AGT^ACT
SfuI 143 TTCGAA TT^CGAA
SmaI 1104 CCCGGG CCC^GGG
SspBI 1696 TGTACA T^GTACA
StyI 3268 CCAAGG C^C[AT][AT]GG
XmaCI 1104 CCCGGG C^CCGGG
XmaI 1104 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AclI 456 AACGTT AA^CGTT
1354 AACGTT
BcnI 1104 CCCGG CC^[GC]GG
1105 CCGGG
BsmBI 586 CGTCTC CGTCTC(1/5)
2126 GAGACG
BsrDI 265 CATTGC GCAATG(2/0)
1886 CATTGC
BssSI 1634 CTCGTG CACGAG(-5/-1)
2352 CTCGTG
Cac8I 269 GCCAGC GC[ACGT]^[ACGT]GC
1986 GCTGGC
EcoT22I 1827 ATGCAT ATGCA^T
2285 ATGCAT
HincII 1518 GTTGAC GT[CT]^[GA]AC
2758 GTTGAC
HindII 1518 GTTGAC GT[CT]^[GA]AC
2758 GTTGAC
MslI 149 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
700 CACCAAAATG
NciI 1104 CCCGG CC^[GC]GG
1105 CCGGG
NsiI 1827 ATGCAT ATGCA^T
2285 ATGCAT
Psp1406I 456 AACGTT AA^CGTT
1354 AACGTT
SfcI 2934 CTGTAG C^T[GA][CT]AG
2975 CTGTAG
SspI 895 AATATT AAT^ATT
2242 AATATT
StuI 2553 AGGCCT AGG^CCT
3101 AGGCCT
XbaI 1668 TCTAGA T^CTAGA
2103 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BalI BbsI BbvI
BclI BfaI BfrI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cfr13I DdeI
DpnII DraI EaeI EarI Eco81I
EcoRII FbaI Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII