##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BseAI BsiWI BspEI BspHI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2685 GACGTC GACGT^C
AgeI 37 ACCGGT A^CCGGT
AlwNI 2860 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BbeI 126 GGCGCC GGCGC^C
BbsI 2532 GTCTTC GAAGAC(2/6)
BglI 180 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2502 AGATCT A^GATCT
BpuAI 2532 GTCTTC GAAGAC(2/6)
BsgI 2235 CTGCAC GTGCAG(16/14)
BsiEI 39 CGGTCG CG[GA][CT]^CG
BsmBI 2683 GAGACG CGTCTC(1/5)
BspDI 1593 ATCGAT AT^CGAT
BsrFI 37 ACCGGT [GA]^CCGG[CT]
BstAPI 2095 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 14 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 37 ACCGGT [GA]^CCGG[CT]
ClaI 1593 ATCGAT AT^CGAT
DraII 263 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 3567 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3735 CCATGG C^C[GA][CT]GG
EcoNI 1172 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 263 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 126 GGCGCC [GA]GCGC^[CT]
KasI 126 GGCGCC G^GCGCC
MspA1I 3201 CAGCTG C[AC]G^C[GT]G
NarI 126 GGCGCC GG^CGCC
NcoI 3735 CCATGG C^CATGG
PaeR7I 2680 CTCGAG C^TCGAG
PflMI 368 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 37 ACCGGT A^CCGGT
PstI 3649 CTGCAG CTGCA^G
PvuII 3201 CAGCTG CAG^CTG
SapI 300 GCTCTTC GCTCTTC(1/4)
ScaI 703 AGTACT AGT^ACT
SfoI 126 GGCGCC GGC^GCC
Van91I 368 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2680 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BanI 126 GGCGCC G^G[CT][GA]CC
586 GGTGCC
BlpI 885 GCTAAGC GC^T[ACGT]AGC
2852 GCTCAGC
Bpu1102I 885 GCTAAGC GC^T[ACGT]AGC
2852 GCTCAGC
BsaI 987 GAGACC GGTCTC(1/5)
3636 GAGACC
BsaWI 37 ACCGGT [AT]^CCGG[AT]
1737 ACCGGA
BsrBI 111 CCGCTC CCGCTC(-3/-3)
250 GAGCGG
BstYI 2502 AGATCT [GA]^GATC[CT]
2627 GGATCT
CelII 885 GCTAAGC GC^T[ACGT]AGC
2852 GCTCAGC
EarI 301 CTCTTC CTCTTC(1/4)
1236 GAAGAG
EcoT22I 1789 ATGCAT ATGCA^T
3248 ATGCAT
HgaI 107 GACGC GACGC(5/10)
50 GCGTC
HincII 786 GTTGAC GT[CT]^[GA]AC
2588 GTCAAC
HindII 786 GTTGAC GT[CT]^[GA]AC
2588 GTCAAC
Ksp632I 301 CTCTTC CTCTTC(1/4)
1236 GAAGAG
MflI 2502 AGATCT [GA]^GATC[CT]
2627 GGATCT
NsiI 1789 ATGCAT ATGCA^T
3248 ATGCAT
NspI 70 ACATGC [GA]CATG^[CT]
2592 ACATGC
SacI 277 GAGCTC GAGCT^C
1364 GAGCTC
SspI 3495 AATATT AAT^ATT
3870 AATATT
StuI 1435 AGGCCT AGG^CCT
2194 AGGCCT
TaqI 1594 TCGA T^CGA
2681 TCGA
TthHB8I 1594 TCGA T^CGA
2681 TCGA
XbaI 3451 TCTAGA T^CTAGA
3911 TCTAGA
XhoII 2502 AGATCT [GA]^GATC[CT]
2627 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmaCI XmaI XmnI