##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AspI AviII BamHI BbrPI
BbvCI BcgI BfrI BglI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI FspI HgaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 1322 GGCGCC G[GA]^CG[CT]C
AhdI 282 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1269 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 886 CCTAAGG CC^T[ACGT]AGG
AspEI 282 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 379 CCTAGG C^CTAGG
BbeI 1322 GGCGCC GGCGC^C
BciVI 2838 GGATAC GTATCC(6/5)
BclI 3296 TGATCA T^GATCA
BglII 593 AGATCT A^GATCT
BlnI 379 CCTAGG C^CTAGG
BsaHI 1322 GGCGCC G[GA]^CG[CT]C
BspLU11I 1286 ACATGT A^CATGT
BssSI 113 CTCGTG CACGAG(-5/-1)
BstAPI 645 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 886 CCTAAGG CC^T[ACGT]AGG
CfoI 1323 GCGC GCG^C
Eam1105I 282 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 886 CCTAAGG CC^T[ACGT]AGG
EcoNI 315 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 450 CCCGC CCCGC(4/6)
FbaI 3296 TGATCA T^GATCA
HaeII 1322 GGCGCC [GA]GCGC^[CT]
HhaI 1323 GCGC GCG^C
Hin1I 1322 GGCGCC G[GA]^CG[CT]C
HinP1I 1323 GCGC G^CGC
HindIII 3004 AAGCTT A^AGCTT
HpaI 4556 GTTAAC GTT^AAC
KasI 1322 GGCGCC G^GCGCC
NarI 1322 GGCGCC GG^CGCC
NheI 453 GCTAGC G^CTAGC
PflMI 2070 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 703 GAGCTC GAGCT^C
SalI 1043 GTCGAC G^TCGAC
ScaI 720 AGTACT AGT^ACT
SfoI 1322 GGCGCC GGC^GCC
StuI 1968 AGGCCT AGG^CCT
SwaI 4776 ATTTAAAT ATTT^AAAT
Van91I 2070 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3478 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3988 GGTACC G^GTACC
4250 GGTACC
AflIII 286 ACGTGT A^C[GA][CT]GT
1286 ACATGT
AseI 2420 ATTAAT AT^TAAT
4821 ATTAAT
AsnI 2420 ATTAAT AT^TAAT
4821 ATTAAT
Asp718I 3988 GGTACC G^GTACC
4250 GGTACC
BalI 1477 TGGCCA TGG^CCA
1874 TGGCCA
BlpI 333 GCTGAGC GC^T[ACGT]AGC
1638 GCTCAGC
Bpu1102I 333 GCTGAGC GC^T[ACGT]AGC
1638 GCTCAGC
Bsp1407I 2623 TGTACA T^GTACA
2631 TGTACA
BspMI 2172 GCAGGT ACCTGC(4/8)
4658 GCAGGT
BsrGI 2623 TGTACA T^GTACA
2631 TGTACA
BstXI 1865 CCATGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2902 CCAGTGTGATGG
BstYI 188 AGATCC [GA]^GATC[CT]
593 AGATCT
CelII 333 GCTGAGC GC^T[ACGT]AGC
1638 GCTCAGC
EaeI 1477 TGGCCA [CT]^GGCC[GA]
1874 TGGCCA
EcoT22I 3439 ATGCAT ATGCA^T
4813 ATGCAT
KpnI 3988 GGTACC GGTAC^C
4250 GGTACC
MflI 188 AGATCC [GA]^GATC[CT]
593 AGATCT
MluNI 1477 TGGCCA TGG^CCA
1874 TGGCCA
MscI 1477 TGGCCA TGG^CCA
1874 TGGCCA
NsiI 3439 ATGCAT ATGCA^T
4813 ATGCAT
PpuMI 1034 GGGACCT [GA]G^G[AT]CC[CT]
1349 AGGACCT
PshAI 816 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1036 GACCTTTGTC
PshBI 2420 ATTAAT AT^TAAT
4821 ATTAAT
SapI 2167 GAAGAGC GCTCTTC(1/4)
558 GCTCTTC
SexAI 3991 ACCTGGT A^CC[AT]GGT
4253 ACCTGGT
SmaI 1424 CCCGGG CCC^GGG
2234 CCCGGG
SmlI 1591 CTTGAG C^T[CT][GA]AG
2814 CTTGAG
SspBI 2623 TGTACA T^GTACA
2631 TGTACA
XcmI 2280 CCAGGGTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4714 CCATTTTTATTGTGG
XhoII 188 AGATCC [GA]^GATC[CT]
593 AGATCT
XmaCI 1424 CCCGGG C^CCGGG
2234 CCCGGG
XmaI 1424 CCCGGG C^CCGGG
2234 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI