##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI ApaI AscI
Asp718I AspEI AviII BamHI BanII
BbeI BbvCI BcgI BfrI BsaWI
BseAI BsiWI BspDI BspEI BspMI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I EcoNI EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 89 GACGCC G[GA]^CG[CT]C
Alw44I 439 GTGCAC G^TGCAC
AocI 3320 CCTAAGG CC^T[ACGT]AGG
ApaLI 439 GTGCAC G^TGCAC
AseI 3027 ATTAAT AT^TAAT
AsnI 3027 ATTAAT AT^TAAT
AspHI 439 GTGCAC G[AT]GC[AT]^C
AspI 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 461 CTCGGG C^[CT]CG[GA]G
BalI 387 TGGCCA TGG^CCA
BbrPI 577 CACGTG CAC^GTG
BciVI 4465 GTATCC GTATCC(6/5)
BlpI 64 GCTGAGC GC^T[ACGT]AGC
BpmI 34 CTCCAG CTGGAG(16/14)
Bpu1102I 64 GCTGAGC GC^T[ACGT]AGC
BsaBI 1073 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 89 GACGCC G[GA]^CG[CT]C
BsaI 1733 GAGACC GGTCTC(1/5)
BsiHKAI 439 GTGCAC G[AT]GC[AT]^C
BsmBI 787 CGTCTC CGTCTC(1/5)
BsoBI 461 CTCGGG C^[CT]CG[GA]G
BspLU11I 1128 ACATGT A^CATGT
BsrBI 448 GAGCGG CCGCTC(-3/-3)
BsrFI 410 ACCGGC [GA]^CCGG[CT]
BssSI 1331 CTCGTG CACGAG(-5/-1)
BstAPI 957 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1159 GGTGACC G^GT[ACGT]ACC
BstPI 1159 GGTGACC G^GT[ACGT]ACC
Bsu36I 3320 CCTAAGG CC^T[ACGT]AGG
CelII 64 GCTGAGC GC^T[ACGT]AGC
Cfr10I 410 ACCGGC [GA]^CCGG[CT]
DraIII 391 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3320 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1159 GGTGACC G^GT[ACGT]ACC
Hin1I 89 GACGCC G[GA]^CG[CT]C
HincII 2001 GTTAAC GT[CT]^[GA]AC
HindII 2001 GTTAAC GT[CT]^[GA]AC
HpaI 2001 GTTAAC GTT^AAC
MamI 1073 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 387 TGGCCA TGG^CCA
MscI 387 TGGCCA TGG^CCA
NheI 1527 GCTAGC G^CTAGC
PflFI 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 577 CACGTG CAC^GTG
PmlI 577 CACGTG CAC^GTG
PpuMI 636 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 179 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3027 ATTAAT AT^TAAT
PvuII 3220 CAGCTG CAG^CTG
SpeI 2246 ACTAGT A^CTAGT
StuI 3429 AGGCCT AGG^CCT
SwaI 3629 ATTTAAAT ATTT^AAAT
Tth111I 89 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 999 AGCGCT AGC^GCT
1323 AGCGCT
Asp700I 1473 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3968 GAAAGAATTC
AvaII 637 GGACC G^G[AT]CC
1170 GGACC
BanI 81 GGCACC G^G[CT][GA]CC
4455 GGTGCC
BglI 92 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1624 GCCTAGGGGGC
BglII 1188 AGATCT A^GATCT
1803 AGATCT
BsaAI 577 CACGTG [CT]AC^GT[GA]
2774 CACGTA
BseRI 849 CTCCTC GAGGAG(10/8)
2430 GAGGAG
Bsp1407I 3693 TGTACA T^GTACA
4546 TGTACA
BsrDI 1141 CATTGC GCAATG(2/0)
2039 CATTGC
BsrGI 3693 TGTACA T^GTACA
4546 TGTACA
DrdI 2652 GACAATAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3462 GACAGGCTAGTC
EagI 21 CGGCCG C^GGCCG
99 CGGCCG
EclXI 21 CGGCCG C^GGCCG
99 CGGCCG
Eco47III 999 AGCGCT AGC^GCT
1323 AGCGCT
Eco52I 21 CGGCCG C^GGCCG
99 CGGCCG
EcoRI 3087 GAATTC G^AATTC
3972 GAATTC
HaeII 999 AGCGCT [GA]GCGC^[CT]
1323 AGCGCT
MspA1I 647 CAGCGG C[AC]G^C[GT]G
3220 CAGCTG
NdeI 2668 CATATG CA^TATG
3484 CATATG
PflMI 208 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACAGAGTGG
ScaI 2620 AGTACT AGT^ACT
4088 AGTACT
SmlI 1003 CTCAAG C^T[CT][GA]AG
1700 CTTGAG
SspBI 3693 TGTACA T^GTACA
4546 TGTACA
Van91I 208 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
390 CCACAGAGTGG
XbaI 2925 TCTAGA T^CTAGA
3940 TCTAGA
XcmI 1146 CCAGGGGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1638 CCAGGGTTCTTGTGG
XmnI 1473 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3968 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvrII
BbsI BbvI BclI BcnI BfaI
BlnI BmyI BpuAI BsaJI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII