##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I AviII AvrII BciVI
BglII BlnI BsaBI BsiWI BsmI
Bsp1407I BspDI BsrGI BssHII BstBI
ClaI CpoI DrdI EcoRI EcoRV
EcoT22I FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1162 GACGTC GACGT^C
AcsI 496 AAATTC [GA]^AATT[CT]
AflII 1993 CTTAAG C^TTAAG
AflIII 4106 ACATGT A^C[GA][CT]GT
AhdI 646 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2541 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1226 AGCGCT AGC^GCT
ApoI 496 AAATTC [GA]^AATT[CT]
AspEI 646 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1004 TGGCCA TGG^CCA
BamHI 116 GGATCC G^GATCC
BbeI 3846 GGCGCC GGCGC^C
BbrPI 3951 CACGTG CAC^GTG
BbvCI 2136 CCTCAGC CCTCAGC(-5/-2)
BcgI 2075 GCAACAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 878 TGATCA T^GATCA
BfrI 1993 CTTAAG C^TTAAG
BlpI 2796 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2796 GCTGAGC GC^T[ACGT]AGC
BsaI 2762 GGTCTC GGTCTC(1/5)
BspHI 3756 TCATGA T^CATGA
BspLU11I 4106 ACATGT A^CATGT
Bst1107I 1074 GTATAC GTA^TAC
BstAPI 2292 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3266 CCAGTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1074 GTATAC GTA^TAC
Bsu36I 2541 CCTCAGG CC^T[ACGT]AGG
CelII 2796 GCTGAGC GC^T[ACGT]AGC
DraI 3186 TTTAAA TTT^AAA
Eam1105I 646 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1226 AGCGCT AGC^GCT
Eco81I 2541 CCTCAGG CC^T[ACGT]AGG
FbaI 878 TGATCA T^GATCA
FseI 2501 GGCCGGCC GGCCGG^CC
HindIII 3196 AAGCTT A^AGCTT
KasI 3846 GGCGCC G^GCGCC
MluNI 1004 TGGCCA TGG^CCA
MscI 1004 TGGCCA TGG^CCA
NarI 3846 GGCGCC GG^CGCC
PflMI 996 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3951 CACGTG CAC^GTG
PmlI 3951 CACGTG CAC^GTG
RcaI 3756 TCATGA T^CATGA
SbfI 1908 CCTGCAGG CCTGCA^GG
SfoI 3846 GGCGCC GGC^GCC
Sse8387I 1908 CCTGCAGG CCTGCA^GG
Van91I 996 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspI 705 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4127 GACTCGGTC
BsmBI 745 GAGACG CGTCTC(1/5)
2224 GAGACG
BsrDI 1455 CATTGC GCAATG(2/0)
257 GCAATG
DraIII 526 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3875 CACCTGGTG
EagI 6 CGGCCG C^GGCCG
34 CGGCCG
EclXI 6 CGGCCG C^GGCCG
34 CGGCCG
Eco52I 6 CGGCCG C^GGCCG
34 CGGCCG
EcoNI 1242 CCTGTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2492 CCTGAGCGAGG
NaeI 615 GCCGGC GCC^GGC
2502 GCCGGC
NcoI 641 CCATGG C^CATGG
3027 CCATGG
NgoMIV 615 GCCGGC G^CCGGC
2502 GCCGGC
PflFI 705 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4127 GACTCGGTC
PshAI 2691 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3383 GACCTTGGTC
PvuII 1273 CAGCTG CAG^CTG
3094 CAGCTG
ScaI 431 AGTACT AGT^ACT
1112 AGTACT
TfiI 66 GATTC G^A[AT]TC
262 GATTC
Tsp509I 497 AATT ^AATT
3180 AATT
Tth111I 705 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4127 GACTCGGTC
XcmI 509 CCAGCTCGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3867 CCAGTCGGCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstEII BstF5I
BstNI BstPI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I XhoII
XmnI