##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI AspI AviII BciVI BclI
BfrI BglII BsaWI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 4153 GGTACC G^GTACC
AhdI 3629 GACACACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2527 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1973 AGCGCT AGC^GCT
Asp700I 2331 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4153 GGTACC G^GTACC
AspEI 3629 GACACACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3871 GGATCC G^GATCC
BbeI 1558 GGCGCC GGCGC^C
BbrPI 4134 CACGTG CAC^GTG
BbsI 2934 GAAGAC GAAGAC(2/6)
BcgI 972 CGAGCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 2934 GAAGAC GAAGAC(2/6)
BsaAI 4134 CACGTG [CT]AC^GT[GA]
BsaBI 276 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 736 GAGACC GGTCTC(1/5)
BsmBI 149 GAGACG CGTCTC(1/5)
BsmI 2619 GAATGC GAATGC(1/-1)
Bsp1407I 3945 TGTACA T^GTACA
BspHI 627 TCATGA T^CATGA
BspLU11I 4146 ACATGT A^CATGT
BspMI 4178 ACCTGC ACCTGC(4/8)
BsrDI 336 CATTGC GCAATG(2/0)
BsrGI 3945 TGTACA T^GTACA
BstAPI 2718 GCACCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4066 GGTTACC G^GT[ACGT]ACC
BstPI 4066 GGTTACC G^GT[ACGT]ACC
Bsu36I 2527 CCTCAGG CC^T[ACGT]AGG
DraI 4471 TTTAAA TTT^AAA
DraIII 518 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3629 GACACACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1973 AGCGCT AGC^GCT
Eco81I 2527 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4066 GGTTACC G^GT[ACGT]ACC
KasI 1558 GGCGCC G^GCGCC
KpnI 4153 GGTACC GGTAC^C
MamI 276 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1558 GGCGCC GG^CGCC
PaeR7I 141 CTCGAG C^TCGAG
PmaCI 4134 CACGTG CAC^GTG
PmlI 4134 CACGTG CAC^GTG
RcaI 627 TCATGA T^CATGA
SbfI 2061 CCTGCAGG CCTGCA^GG
SfiI 1896 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1558 GGCGCC GGC^GCC
Sse8387I 2061 CCTGCAGG CCTGCA^GG
SspBI 3945 TGTACA T^GTACA
XhoI 141 CTCGAG C^TCGAG
XmnI 2331 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 244 GAATTT [GA]^AATT[CT]
2876 AAATTT
AcyI 1558 GGCGCC G[GA]^CG[CT]C
3032 GACGCC
AflIII 4135 ACGTGT A^C[GA][CT]GT
4146 ACATGT
ApoI 244 GAATTT [GA]^AATT[CT]
2876 AAATTT
AvrII 480 CCTAGG C^CTAGG
4301 CCTAGG
BlnI 480 CCTAGG C^CTAGG
4301 CCTAGG
BsaHI 1558 GGCGCC G[GA]^CG[CT]C
3032 GACGCC
BsgI 1795 CTGCAC GTGCAG(16/14)
3370 CTGCAC
BsiEI 193 CGGCCG CG[GA][CT]^CG
694 CGGCCG
BssSI 394 CTCGTG CACGAG(-5/-1)
2086 CTCGTG
EagI 193 CGGCCG C^GGCCG
694 CGGCCG
EclXI 193 CGGCCG C^GGCCG
694 CGGCCG
Eco52I 193 CGGCCG C^GGCCG
694 CGGCCG
Hin1I 1558 GGCGCC G[GA]^CG[CT]C
3032 GACGCC
KspI 27 CCGCGG CCGC^GG
183 CCGCGG
MaeII 1584 ACGT A^CGT
4135 ACGT
SacII 27 CCGCGG CCGC^GG
183 CCGCGG
SapI 932 GCTCTTC GCTCTTC(1/4)
1398 GCTCTTC
SphI 2213 GCATGC GCATG^C
4108 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI