##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI Alw44I ApaLI ApoI AscI
Asp718I AvrII BamHI BclI BlnI
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRI FbaI FseI HgaI HincII
HindII HindIII HpaI KpnI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SmaI SnaBI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 3322 GACGTC GACGT^C
AccII 4 CGCG CG^CG
AflII 1952 CTTAAG C^TTAAG
AflIII 4099 ACATGT A^C[GA][CT]GT
AhdI 4676 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3966 AGCGCT AGC^GCT
AseI 4924 ATTAAT AT^TAAT
AsnI 4924 ATTAAT AT^TAAT
AspEI 4676 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2640 TGCGCA TGC^GCA
BbeI 3996 GGCGCC GGCGC^C
BbrPI 2715 CACGTG CAC^GTG
BciVI 3257 GGATAC GTATCC(6/5)
BfrI 1952 CTTAAG C^TTAAG
BglII 91 AGATCT A^GATCT
BlpI 1272 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1272 GCTCAGC GC^T[ACGT]AGC
BsaBI 2024 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 176 TGTACA T^GTACA
BspLU11I 4099 ACATGT A^CATGT
BsrFI 2561 ACCGGC [GA]^CCGG[CT]
BsrGI 176 TGTACA T^GTACA
BstEII 2650 GGTCACC G^GT[ACGT]ACC
BstPI 2650 GGTCACC G^GT[ACGT]ACC
BstUI 4 CGCG CG^CG
CelII 1272 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2561 ACCGGC [GA]^CCGG[CT]
DraIII 1234 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 48 GACCGCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4676 GACAACTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3966 AGCGCT AGC^GCT
EcoO65I 2650 GGTCACC G^GT[ACGT]ACC
EcoRV 2437 GATATC GAT^ATC
EcoT22I 3146 ATGCAT ATGCA^T
FspI 2640 TGCGCA TGC^GCA
KasI 3996 GGCGCC G^GCGCC
KspI 3 CCGCGG CCGC^GG
MamI 2024 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 4 CGCG CG^CG
NarI 3996 GGCGCC GG^CGCC
NheI 2072 GCTAGC G^CTAGC
NsiI 3146 ATGCAT ATGCA^T
PmaCI 2715 CACGTG CAC^GTG
PmlI 2715 CACGTG CAC^GTG
PshBI 4924 ATTAAT AT^TAAT
SacI 267 GAGCTC GAGCT^C
SacII 3 CCGCGG CCGC^GG
SbfI 4041 CCTGCAGG CCTGCA^GG
ScaI 2198 AGTACT AGT^ACT
SexAI 1102 ACCAGGT A^CC[AT]GGT
SfiI 1317 GGCCTGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3996 GGCGCC GGC^GCC
SpeI 4680 ACTAGT A^CTAGT
Sse8387I 4041 CCTGCAGG CCTGCA^GG
SspBI 176 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 3322 GACGTC G[GA]^CG[CT]C
3996 GGCGCC
Asp700I 128 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3940 GAAAAAGTTC
AspHI 267 GAGCTC G[AT]GC[AT]^C
2749 GTGCTC
BsaAI 2715 CACGTG [CT]AC^GT[GA]
3902 TACGTG
BsaHI 3322 GACGTC G[GA]^CG[CT]C
3996 GGCGCC
BsaWI 228 ACCGGA [AT]^CCGG[AT]
4311 ACCGGA
BsiHKAI 267 GAGCTC G[AT]GC[AT]^C
2749 GTGCTC
BsmI 256 GAATGC GAATGC(1/-1)
1335 GAATGC
BspHI 2763 TCATGA T^CATGA
4341 TCATGA
BsrDI 1917 GCAATG GCAATG(2/0)
3081 GCAATG
BssSI 4016 CACGAG CACGAG(-5/-1)
524 CTCGTG
Hin1I 3322 GACGTC G[GA]^CG[CT]C
3996 GGCGCC
PpuMI 1665 AGGTCCT [GA]G^G[AT]CC[CT]
2013 AGGTCCC
PvuII 2532 CAGCTG CAG^CTG
3271 CAGCTG
RcaI 2763 TCATGA T^CATGA
4341 TCATGA
SapI 2605 GAAGAGC GCTCTTC(1/4)
4817 GAAGAGC
Tsp45I 2651 GTCAC ^GT[GC]AC
3873 GTCAC
XmnI 128 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3940 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII