##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AscI Asp700I Asp718I BamHI BciVI
BclI BfrI BglII BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrGI Bst1107I BstBI BstYI
BstZ17I ClaI CpoI DrdI EcoRI
EcoRV FbaI HindIII HpaI KpnI
MamI MfeI MflI MroI MunI
NdeI NotI NruI NspV PacI
PmeI PshAI Psp1406I PvuI RsrII
SexAI SfuI SnaBI SpeI SspBI
SspI SwaI XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1486 GTCGAC GT^[AC][GT]AC
AcsI 5520 AAATTC [GA]^AATT[CT]
AgeI 5799 ACCGGT A^CCGGT
AlwI 3414 GGATC GGATC(4/5)
ApoI 5520 AAATTC [GA]^AATT[CT]
AseI 5786 ATTAAT AT^TAAT
AsnI 5786 ATTAAT AT^TAAT
BspHI 2159 TCATGA T^CATGA
FseI 5049 GGCCGGCC GGCCGG^CC
MluI 5224 ACGCGT A^CGCGT
NheI 909 GCTAGC G^CTAGC
PinAI 5799 ACCGGT A^CCGGT
PshBI 5786 ATTAAT AT^TAAT
RcaI 2159 TCATGA T^CATGA
SalI 1486 GTCGAC G^TCGAC
SapI 4204 GCTCTTC GCTCTTC(1/4)
SbfI 1473 CCTGCAGG CCTGCA^GG
ScaI 5767 AGTACT AGT^ACT
SfiI 4291 GGCCGAAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 5798 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1473 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 381 ACACGT A^C[GA][CT]GT
5224 ACGCGT
AhdI 2688 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3539 GACAAGCTGTC
Alw44I 234 GTGCAC G^TGCAC
2246 GTGCAC
Aor51HI 1697 AGCGCT AGC^GCT
4508 AGCGCT
ApaLI 234 GTGCAC G^TGCAC
2246 GTGCAC
AspEI 2688 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3539 GACAAGCTGTC
AviII 3249 TGCGCA TGC^GCA
3378 TGCGCA
AvrII 349 CCTAGG C^CTAGG
1525 CCTAGG
BbeI 3033 GGCGCC GGCGC^C
4487 GGCGCC
BbsI 1800 GAAGAC GAAGAC(2/6)
3042 GTCTTC
BcgI 3490 CGAGATTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2406 GCAGGTGGCTCG
BlnI 349 CCTAGG C^CTAGG
1525 CCTAGG
BpuAI 1800 GAAGAC GAAGAC(2/6)
3042 GTCTTC
BsaWI 2302 ACCGGA [AT]^CCGG[AT]
5799 ACCGGT
BsmI 5731 GCATTC GAATGC(1/-1)
5780 GCATTC
BssSI 3488 CACGAG CACGAG(-5/-1)
4058 CACGAG
Eam1105I 2688 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3539 GACAAGCTGTC
Eco47III 1697 AGCGCT AGC^GCT
4508 AGCGCT
EcoT22I 4874 ATGCAT ATGCA^T
5749 ATGCAT
FspI 3249 TGCGCA TGC^GCA
3378 TGCGCA
HincII 1000 GTTGAC GT[CT]^[GA]AC
1486 GTCGAC
HindII 1000 GTTGAC GT[CT]^[GA]AC
1486 GTCGAC
KasI 3033 GGCGCC G^GCGCC
4487 GGCGCC
MslI 1412 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5170 CACCACGGTG
NaeI 4514 GCCGGC GCC^GGC
5050 GCCGGC
NarI 3033 GGCGCC GG^CGCC
4487 GGCGCC
NgoMIV 4514 GCCGGC G^CCGGC
5050 GCCGGC
NsiI 4874 ATGCAT ATGCA^T
5749 ATGCAT
PaeR7I 3281 CTCGAG C^TCGAG
3290 CTCGAG
SfoI 3033 GGCGCC GGC^GCC
4487 GGCGCC
XhoI 3281 CTCGAG C^TCGAG
3290 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XmaCI
XmaI