##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AviII BanI
BbeI BbvCI BcgI BclI BfrI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BssHII
BssSI BstEII BstPI BstUI ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HgaI Hin1I KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SphI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 3355 AACGTT AA^CGTT
Alw44I 3655 GTGCAC G^TGCAC
AlwNI 2675 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3408 CCTAAGG CC^T[ACGT]AGG
ApaLI 3655 GTGCAC G^TGCAC
Asp700I 1982 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3655 GTGCAC G[AT]GC[AT]^C
AvaI 3732 CCCGAG C^[CT]CG[GA]G
AvrII 2107 CCTAGG C^CTAGG
BanII 2271 GAGCCC G[GA]GC[CT]^C
BbrPI 2039 CACGTG CAC^GTG
BbsI 2306 GAAGAC GAAGAC(2/6)
BcnI 1609 CCGGG CC^[GC]GG
BlnI 2107 CCTAGG C^CTAGG
BlpI 3787 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3787 GCTAAGC GC^T[ACGT]AGC
BpuAI 2306 GAAGAC GAAGAC(2/6)
BsiEI 155 CGGTCG CG[GA][CT]^CG
BsiHKAI 3655 GTGCAC G[AT]GC[AT]^C
BsoBI 3732 CCCGAG C^[CT]CG[GA]G
BsrBI 2000 GAGCGG CCGCTC(-3/-3)
Bst1107I 2995 GTATAC GTA^TAC
BstBI 1972 TTCGAA TT^CGAA
BstXI 1481 CCATGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2995 GTATAC GTA^TAC
Bsu36I 3408 CCTAAGG CC^T[ACGT]AGG
CelII 3787 GCTAAGC GC^T[ACGT]AGC
CfoI 780 GCGC GCG^C
DrdI 2388 GACACCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3408 CCTAAGG CC^T[ACGT]AGG
EcoRI 994 GAATTC G^AATTC
EcoT22I 1726 ATGCAT ATGCA^T
HaeII 779 GGCGCT [GA]GCGC^[CT]
HhaI 780 GCGC GCG^C
HinP1I 780 GCGC G^CGC
HindIII 624 AAGCTT A^AGCTT
HpaI 1295 GTTAAC GTT^AAC
MfeI 2180 CAATTG C^AATTG
MunI 2180 CAATTG C^AATTG
NciI 1609 CCGGG CC^[GC]GG
NsiI 1726 ATGCAT ATGCA^T
NspV 1972 TTCGAA TT^CGAA
PmaCI 2039 CACGTG CAC^GTG
PmlI 2039 CACGTG CAC^GTG
PpuMI 2356 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3355 AACGTT AA^CGTT
SfuI 1972 TTCGAA TT^CGAA
SmlI 903 CTCAAG C^T[CT][GA]AG
SnaBI 366 TACGTA TAC^GTA
SpeI 401 ACTAGT A^CTAGT
XmnI 1982 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 1619 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2283 GACTATGTC
BciVI 3634 GGATAC GTATCC(6/5)
3046 GTATCC
BglI 685 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3731 GCCCGAGGGGC
BsaAI 366 TACGTA [CT]AC^GT[GA]
2039 CACGTG
BsaI 2008 GAGACC GGTCTC(1/5)
2841 GAGACC
BseRI 934 CTCCTC GAGGAG(10/8)
1917 GAGGAG
BsmI 317 GAATGC GAATGC(1/-1)
3805 GCATTC
BsrFI 25 ACCGGC [GA]^CCGG[CT]
153 GCCGGT
Cfr10I 25 ACCGGC [GA]^CCGG[CT]
153 GCCGGT
DraI 1242 TTTAAA TTT^AAA
3611 TTTAAA
DraII 2356 AGGACCT [GA]G^G[ACGT]CC[CT]
3737 GGGGCCT
EcoO109I 2356 AGGACCT [GA]G^G[ACGT]CC[CT]
3737 GGGGCCT
HincII 341 GTCAAC GT[CT]^[GA]AC
1295 GTTAAC
HindII 341 GTCAAC GT[CT]^[GA]AC
1295 GTTAAC
NdeI 382 CATATG CA^TATG
1991 CATATG
PflFI 1619 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2283 GACTATGTC
PflMI 1946 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2674 CCAGGTGCTGG
PstI 117 CTGCAG CTGCA^G
3451 CTGCAG
SexAI 1072 ACCAGGT A^CC[AT]GGT
1945 ACCAGGT
StuI 373 AGGCCT AGG^CCT
3405 AGGCCT
Tth111I 1619 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2283 GACTATGTC
Van91I 1946 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2674 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BalI BamHI BbvI BfaI
BglII BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII