##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspHI Bst1107I BstXI BstZ17I
ClaI CpoI Eam1105I EcoRV FseI
FspI KasI KpnI MamI MluI
MroI NarI NdeI NheI NruI
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 138 GGCGTC G[GA]^CG[CT]C
AgeI 2162 ACCGGT A^CCGGT
AocI 160 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1319 AGCGCT AGC^GCT
ApaI 2655 GGGCCC GGGCC^C
AspHI 3828 GAGCTC G[AT]GC[AT]^C
AvaI 40 CCCGAG C^[CT]CG[GA]G
BalI 1499 TGGCCA TGG^CCA
BclI 821 TGATCA T^GATCA
BglII 2805 AGATCT A^GATCT
BsaHI 138 GGCGTC G[GA]^CG[CT]C
BsaWI 2162 ACCGGT [AT]^CCGG[AT]
BsiHKAI 3828 GAGCTC G[AT]GC[AT]^C
BsmI 2010 GAATGC GAATGC(1/-1)
BsoBI 40 CCCGAG C^[CT]CG[GA]G
BsrDI 789 CATTGC GCAATG(2/0)
BssHII 20 GCGCGC G^CGCGC
BstAPI 2494 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 887 TTCGAA TT^CGAA
BstEII 1705 GGTGACC G^GT[ACGT]ACC
BstPI 1705 GGTGACC G^GT[ACGT]ACC
Bsu36I 160 CCTAAGG CC^T[ACGT]AGG
DraIII 3941 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2829 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1319 AGCGCT AGC^GCT
Eco81I 160 CCTAAGG CC^T[ACGT]AGG
EcoNI 160 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1705 GGTGACC G^GT[ACGT]ACC
EcoRI 1216 GAATTC G^AATTC
FbaI 821 TGATCA T^GATCA
Hin1I 138 GGCGTC G[GA]^CG[CT]C
HpaI 1398 GTTAAC GTT^AAC
KspI 131 CCGCGG CCGC^GG
MfeI 1124 CAATTG C^AATTG
MluNI 1499 TGGCCA TGG^CCA
MscI 1499 TGGCCA TGG^CCA
MunI 1124 CAATTG C^AATTG
NaeI 32 GCCGGC GCC^GGC
NcoI 4081 CCATGG C^CATGG
NgoMIV 32 GCCGGC G^CCGGC
NotI 1 GCGGCCGC GC^GGCCGC
NspV 887 TTCGAA TT^CGAA
PinAI 2162 ACCGGT A^CCGGT
PshAI 1900 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 4383 CTGCAG CTGCA^G
SacI 3828 GAGCTC GAGCT^C
SacII 131 CCGCGG CCGC^GG
ScaI 3382 AGTACT AGT^ACT
SfuI 887 TTCGAA TT^CGAA
SspI 3791 AATATT AAT^ATT
StuI 2303 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 21 CGCG CG^CG
132 CGCG
AflIII 482 ACATGT A^C[GA][CT]GT
3995 ACATGT
AspI 1609 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCTTGTC
BanI 1970 GGTGCC G^G[CT][GA]CC
2043 GGCACC
BglI 1877 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2326 GCCAGTGAGGC
BlpI 24 GCTGAGC GC^T[ACGT]AGC
2511 GCTCAGC
Bpu1102I 24 GCTGAGC GC^T[ACGT]AGC
2511 GCTCAGC
BsgI 2549 GTGCAG GTGCAG(16/14)
3091 GTGCAG
BsiEI 2 CGGCCG CG[GA][CT]^CG
51 CGGCCG
Bsp1407I 3784 TGTACA T^GTACA
4175 TGTACA
BspLU11I 482 ACATGT A^CATGT
3995 ACATGT
BsrGI 3784 TGTACA T^GTACA
4175 TGTACA
BssSI 3305 CACGAG CACGAG(-5/-1)
2665 CTCGTG
BstUI 21 CGCG CG^CG
132 CGCG
CelII 24 GCTGAGC GC^T[ACGT]AGC
2511 GCTCAGC
EagI 2 CGGCCG C^GGCCG
51 CGGCCG
EclXI 2 CGGCCG C^GGCCG
51 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
51 CGGCCG
EcoT22I 2012 ATGCAT ATGCA^T
3232 ATGCAT
HgaI 1990 GACGC GACGC(5/10)
139 GCGTC
HindIII 2241 AAGCTT A^AGCTT
3202 AAGCTT
MaeII 555 ACGT A^CGT
1473 ACGT
MvnI 21 CGCG CG^CG
132 CGCG
NsiI 2012 ATGCAT ATGCA^T
3232 ATGCAT
PflFI 1609 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCTTGTC
SspBI 3784 TGTACA T^GTACA
4175 TGTACA
TaqI 795 TCGA T^CGA
888 TCGA
Tth111I 1609 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3004 GACCTTGTC
TthHB8I 795 TCGA T^CGA
888 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI