##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AclI AflII AgeI ApaI AseI
AsnI Asp700I BamHI BbeI BbrPI
BciVI BfrI BsiWI BspDI BspHI
BstBI ClaI CpoI DrdI EcoRV
FseI HpaI KasI MfeI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 5842 GTATAC GT^[AC][GT]AC
AccIII 1253 TCCGGA T^CCGGA
AocI 2571 CCTCAGG CC^T[ACGT]AGG
AscI 23 GGCGCGCC GG^CGCGCC
AspI 501 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3192 TGCGCA TGC^GCA
AvrII 4038 CCTAGG C^CTAGG
BclI 5689 TGATCA T^GATCA
BglII 1905 AGATCT A^GATCT
BlnI 4038 CCTAGG C^CTAGG
BlpI 3236 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3236 GCTGAGC GC^T[ACGT]AGC
BsaWI 1253 TCCGGA [AT]^CCGG[AT]
BseAI 1253 TCCGGA T^CCGGA
BspEI 1253 TCCGGA T^CCGGA
BssHII 24 GCGCGC G^CGCGC
BssSI 5708 CACGAG CACGAG(-5/-1)
Bst1107I 5842 GTATAC GTA^TAC
BstZ17I 5842 GTATAC GTA^TAC
Bsu36I 2571 CCTCAGG CC^T[ACGT]AGG
CelII 3236 GCTGAGC GC^T[ACGT]AGC
DraI 5024 TTTAAA TTT^AAA
DraIII 2309 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2571 CCTCAGG CC^T[ACGT]AGG
EcoT22I 4026 ATGCAT ATGCA^T
FbaI 5689 TGATCA T^GATCA
FspI 3192 TGCGCA TGC^GCA
HincII 2577 GTCAAC GT[CT]^[GA]AC
HindII 2577 GTCAAC GT[CT]^[GA]AC
KspI 30 CCGCGG CCGC^GG
MluI 971 ACGCGT A^CGCGT
MroI 1253 TCCGGA T^CCGGA
NsiI 4026 ATGCAT ATGCA^T
PaeR7I 4676 CTCGAG C^TCGAG
PflFI 501 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 30 CCGCGG CCGC^GG
SapI 5748 GCTCTTC GCTCTTC(1/4)
SbfI 2795 CCTGCAGG CCTGCA^GG
Sse8387I 2795 CCTGCAGG CCTGCA^GG
SspI 1056 AATATT AAT^ATT
SwaI 5023 ATTTAAAT ATTT^AAAT
Tth111I 501 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4279 TCTAGA T^CTAGA
XhoI 4676 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1026 GACGTC GACGT^C
2528 GACGTC
Acc65I 1399 GGTACC G^GTACC
4233 GGTACC
AhdI 2586 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4134 GACAAGGCGTC
Alw44I 1332 GTGCAC G^TGCAC
4696 GTGCAC
ApaLI 1332 GTGCAC G^TGCAC
4696 GTGCAC
Asp718I 1399 GGTACC G^GTACC
4233 GGTACC
AspEI 2586 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4134 GACAAGGCGTC
BbvCI 3566 CCTCAGC CCTCAGC(-5/-2)
4588 CCTCAGC
BcgI 1054 CGAATATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2560 CGACGGCGCTGC
BsaBI 3045 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3126 GATGGGCATC
BsmI 1109 GAATGC GAATGC(1/-1)
1729 GCATTC
Bsp1407I 2836 TGTACA T^GTACA
3879 TGTACA
BsrFI 455 ACCGGC [GA]^CCGG[CT]
2325 ACCGGC
BsrGI 2836 TGTACA T^GTACA
3879 TGTACA
BstEII 4640 GGTGACC G^GT[ACGT]ACC
5175 GGTGACC
BstPI 4640 GGTGACC G^GT[ACGT]ACC
5175 GGTGACC
Cfr10I 455 ACCGGC [GA]^CCGG[CT]
2325 ACCGGC
EagI 257 CGGCCG C^GGCCG
1547 CGGCCG
Eam1105I 2586 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4134 GACAAGGCGTC
EclXI 257 CGGCCG C^GGCCG
1547 CGGCCG
Eco52I 257 CGGCCG C^GGCCG
1547 CGGCCG
EcoO65I 4640 GGTGACC G^GT[ACGT]ACC
5175 GGTGACC
KpnI 1399 GGTACC GGTAC^C
4233 GGTACC
MamI 3045 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3126 GATGGGCATC
NcoI 3037 CCATGG C^CATGG
3061 CCATGG
PshAI 2334 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4408 GACAGTGGTC
PstI 2796 CTGCAG CTGCA^G
4119 CTGCAG
SmaI 4149 CCCGGG CCC^GGG
5314 CCCGGG
SphI 680 GCATGC GCATG^C
712 GCATGC
SspBI 2836 TGTACA T^GTACA
3879 TGTACA
XcmI 217 CCATGCCAATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
941 CCATCAAATTTTTGG
XmaCI 4149 CCCGGG C^CCGGG
5314 CCCGGG
XmaI 4149 CCCGGG C^CCGGG
5314 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI Aor51HI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII