##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BbsI BfrI
BlnI BpuAI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV FseI FspI
HaeII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PpuMI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 3866 GACGTC GACGT^C
AlwNI 1799 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 95 CCTGAGG CC^T[ACGT]AGG
ApaI 751 GGGCCC GGGCC^C
BanI 3779 GGCACC G^G[CT][GA]CC
BbrPI 2485 CACGTG CAC^GTG
BcgI 5014 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3835 GCCGTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2299 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2299 GCTGAGC GC^T[ACGT]AGC
BsaAI 2485 CACGTG [CT]AC^GT[GA]
BsaWI 166 TCCGGT [AT]^CCGG[AT]
BsmBI 3822 GAGACG CGTCTC(1/5)
Bsp1407I 3549 TGTACA T^GTACA
BsrGI 3549 TGTACA T^GTACA
Bst1107I 4410 GTATAC GTA^TAC
BstEII 1694 GGTTACC G^GT[ACGT]ACC
BstPI 1694 GGTTACC G^GT[ACGT]ACC
BstZ17I 4410 GTATAC GTA^TAC
Bsu36I 95 CCTGAGG CC^T[ACGT]AGG
CelII 2299 GCTGAGC GC^T[ACGT]AGC
Eco81I 95 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1694 GGTTACC G^GT[ACGT]ACC
FauI 3880 CCCGC CCCGC(4/6)
HgaI 3791 GACGC GACGC(5/10)
HindIII 4555 AAGCTT A^AGCTT
MfeI 5274 CAATTG C^AATTG
MunI 5274 CAATTG C^AATTG
NdeI 3528 CATATG CA^TATG
PflMI 902 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2485 CACGTG CAC^GTG
PmlI 2485 CACGTG CAC^GTG
PshAI 3245 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 5651 AGTACT AGT^ACT
SmaI 3727 CCCGGG CCC^GGG
SspBI 3549 TGTACA T^GTACA
StuI 2132 AGGCCT AGG^CCT
Van91I 902 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1730 CCATTTACGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3727 CCCGGG C^CCGGG
XmaI 3727 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 4410 GTATAC GT^[AC][GT]AC
4604 GTAGAC
AcyI 3791 GACGCC G[GA]^CG[CT]C
3866 GACGTC
AflIII 1930 ACATGT A^C[GA][CT]GT
2243 ACATGT
Alw44I 3688 GTGCAC G^TGCAC
4249 GTGCAC
ApaLI 3688 GTGCAC G^TGCAC
4249 GTGCAC
BamHI 1634 GGATCC G^GATCC
3134 GGATCC
BsaHI 3791 GACGCC G[GA]^CG[CT]C
3866 GACGTC
BspLU11I 1930 ACATGT A^CATGT
2243 ACATGT
BspMI 920 ACCTGC ACCTGC(4/8)
402 GCAGGT
BstAPI 242 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
725 GCAAGACGTGC
DraII 750 AGGGCCC [GA]G^G[ACGT]CC[CT]
751 GGGCCCT
DrdI 1129 GACACTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4346 GACTAATAAGTC
EcoO109I 750 AGGGCCC [GA]G^G[ACGT]CC[CT]
751 GGGCCCT
EcoRI 2498 GAATTC G^AATTC
5161 GAATTC
EcoT22I 3525 ATGCAT ATGCA^T
4390 ATGCAT
Hin1I 3791 GACGCC G[GA]^CG[CT]C
3866 GACGTC
NsiI 3525 ATGCAT ATGCA^T
4390 ATGCAT
SacI 1521 GAGCTC GAGCT^C
1554 GAGCTC
SapI 4517 GAAGAGC GCTCTTC(1/4)
767 GCTCTTC
SmlI 1536 CTCAAG C^T[CT][GA]AG
3211 CTCAAG
SwaI 1261 ATTTAAAT ATTT^AAAT
5260 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AspI AvaI AvaII BalI
BanII BbvCI BbvI BciVI BclI
BcnI BfaI BglII BmyI BpmI
BsaBI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraIII DsaI EaeI
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XhoII
XmnI