##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
BamHI BclI BfrI BseAI BsiWI
BspDI BspEI BspLU11I BssHII BstBI
ClaI CpoI Eco47III EcoRI EcoRV
FbaI HindIII MfeI MluI MroI
MunI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 5177 GGTACC G^GTACC
AhdI 2010 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 5177 GGTACC G^GTACC
AspEI 2010 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 66 CCTAGG C^CTAGG
BglII 3880 AGATCT A^GATCT
BlnI 66 CCTAGG C^CTAGG
BsrDI 1090 GCAATG GCAATG(2/0)
Bst1107I 6752 GTATAC GTA^TAC
BstZ17I 6752 GTATAC GTA^TAC
DrdI 5394 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2010 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4778 ATGCAT ATGCA^T
KpnI 5177 GGTACC GGTAC^C
NdeI 319 CATATG CA^TATG
NheI 3555 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
NsiI 4778 ATGCAT ATGCA^T
PaeR7I 4684 CTCGAG C^TCGAG
PshAI 5588 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2157 GTCGAC G^TCGAC
SapI 302 GAAGAGC GCTCTTC(1/4)
ScaI 5111 AGTACT AGT^ACT
SexAI 510 ACCAGGT A^CC[AT]GGT
XhoI 4684 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 3346 CCTCAGG CC^T[ACGT]AGG
4950 CCTCAGG
BbeI 1533 GGCGCC GGCGC^C
2811 GGCGCC
BcgI 3514 GCACAGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5277 GCACACACTTCG
BsaBI 2870 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3881 GATCTACATC
BsaWI 861 ACCGGA [AT]^CCGG[AT]
3217 ACCGGA
BsmI 488 GAATGC GAATGC(1/-1)
2036 GAATGC
BspHI 3085 TCATGA T^CATGA
3331 TCATGA
BstAPI 1920 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6446 GCAGGCCTTGC
BstEII 3296 GGTCACC G^GT[ACGT]ACC
3377 GGTGACC
BstPI 3296 GGTCACC G^GT[ACGT]ACC
3377 GGTGACC
Bsu36I 3346 CCTCAGG CC^T[ACGT]AGG
4950 CCTCAGG
DraI 5067 TTTAAA TTT^AAA
6365 TTTAAA
Eco81I 3346 CCTCAGG CC^T[ACGT]AGG
4950 CCTCAGG
EcoO65I 3296 GGTCACC G^GT[ACGT]ACC
3377 GGTGACC
HpaI 4679 GTTAAC GTT^AAC
5644 GTTAAC
KasI 1533 GGCGCC G^GCGCC
2811 GGCGCC
MamI 2870 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3881 GATCTACATC
NarI 1533 GGCGCC GG^CGCC
2811 GGCGCC
PstI 3912 CTGCAG CTGCA^G
6405 CTGCAG
RcaI 3085 TCATGA T^CATGA
3331 TCATGA
SfiI 4038 GGCCTGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4291 GGCCAGGGTGGCC
SfoI 1533 GGCGCC GGC^GCC
2811 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FseI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI