##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BcgI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BspDI BspEI BssHII
BssSI BstAPI BstEII BstPI CelII
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV FseI FspI KpnI KspI
MamI MluI MluNI MroI MscI
NotI NruI PaeR7I PinAI PshAI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SgrAI SphI SwaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1117 GTATAC GT^[AC][GT]AC
Alw44I 2215 GTGCAC G^TGCAC
AlwNI 166 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1459 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1672 AGCGCT AGC^GCT
ApaLI 2215 GTGCAC G^TGCAC
AspI 1346 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 913 GGATCC G^GATCC
BbrPI 5224 CACGTG CAC^GTG
BglII 2714 AGATCT A^GATCT
BsiEI 1165 CGACCG CG[GA][CT]^CG
Bst1107I 1117 GTATAC GTA^TAC
BstBI 5774 TTCGAA TT^CGAA
BstZ17I 1117 GTATAC GTA^TAC
Bsu36I 1459 CCTCAGG CC^T[ACGT]AGG
DraII 3416 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 1672 AGCGCT AGC^GCT
Eco81I 1459 CCTCAGG CC^T[ACGT]AGG
EcoO109I 3416 AGGACCT [GA]G^G[ACGT]CC[CT]
HindIII 3269 AAGCTT A^AGCTT
MfeI 908 CAATTG C^AATTG
MunI 908 CAATTG C^AATTG
NspV 5774 TTCGAA TT^CGAA
PacI 4817 TTAATTAA TTAAT^TAA
PflFI 1346 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2143 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5224 CACGTG CAC^GTG
PmeI 5496 GTTTAAAC GTTT^AAAC
PmlI 5224 CACGTG CAC^GTG
PpuMI 3416 AGGACCT [GA]G^G[AT]CC[CT]
SacI 4271 GAGCTC GAGCT^C
SbfI 1690 CCTGCAGG CCTGCA^GG
ScaI 3515 AGTACT AGT^ACT
SfuI 5774 TTCGAA TT^CGAA
SmaI 349 CCCGGG CCC^GGG
SnaBI 3075 TACGTA TAC^GTA
Sse8387I 1690 CCTGCAGG CCTGCA^GG
StuI 3186 AGGCCT AGG^CCT
Tth111I 1346 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2143 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 349 CCCGGG C^CCGGG
XmaI 349 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 2487 CTTAAG C^TTAAG
2954 CTTAAG
BbsI 1352 GTCTTC GAAGAC(2/6)
4947 GTCTTC
BbvCI 324 CCTCAGC CCTCAGC(-5/-2)
204 GCTGAGG
BfrI 2487 CTTAAG C^TTAAG
2954 CTTAAG
BpuAI 1352 GTCTTC GAAGAC(2/6)
4947 GTCTTC
BsgI 226 GTGCAG GTGCAG(16/14)
567 GTGCAG
BsmI 1874 GAATGC GAATGC(1/-1)
5688 GAATGC
BspHI 2739 TCATGA T^CATGA
3431 TCATGA
BsrDI 1319 GCAATG GCAATG(2/0)
1705 GCAATG
BstXI 556 CCATTAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1554 CCATGGCTCTGG
EcoRI 2232 GAATTC G^AATTC
3603 GAATTC
EcoT22I 1105 ATGCAT ATGCA^T
5188 ATGCAT
NcoI 1554 CCATGG C^CATGG
3443 CCATGG
NdeI 4585 CATATG CA^TATG
5534 CATATG
NheI 405 GCTAGC G^CTAGC
5097 GCTAGC
NsiI 1105 ATGCAT ATGCA^T
5188 ATGCAT
PvuII 202 CAGCTG CAG^CTG
3494 CAGCTG
RcaI 2739 TCATGA T^CATGA
3431 TCATGA
SpeI 2344 ACTAGT A^CTAGT
4156 ACTAGT
TaqI 1164 TCGA T^CGA
5775 TCGA
TthHB8I 1164 TCGA T^CGA
5775 TCGA
XbaI 4613 TCTAGA T^CTAGA
4623 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApaI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbeI BbvI BciVI
BclI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EarI EcoNI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PshBI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII