##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AvrII BamHI BanI BbeI BfrI
BlnI BlpI Bpu1102I BseAI BsiWI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FseI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PvuI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 4240 CGCG CG^CG
AclI 3358 AACGTT AA^CGTT
Alw44I 2228 GTGCAC G^TGCAC
AlwNI 3904 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4889 GGGCCC GGGCC^C
ApaLI 2228 GTGCAC G^TGCAC
Asp700I 2763 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3704 TGCGCA TGC^GCA
BbsI 2118 GTCTTC GAAGAC(2/6)
BbvCI 3366 CCTCAGC CCTCAGC(-5/-2)
BcgI 2676 GCAACAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 516 GGATAC GTATCC(6/5)
BglII 2549 AGATCT A^GATCT
BpuAI 2118 GTCTTC GAAGAC(2/6)
BsaWI 967 TCCGGT [AT]^CCGG[AT]
BsmBI 4617 CGTCTC CGTCTC(1/5)
BsmI 186 GCATTC GAATGC(1/-1)
Bsp1407I 1977 TGTACA T^GTACA
BspDI 3688 ATCGAT AT^CGAT
BspHI 3456 TCATGA T^CATGA
BsrGI 1977 TGTACA T^GTACA
BstUI 4240 CGCG CG^CG
ClaI 3688 ATCGAT AT^CGAT
CpoI 3994 CGGACCG CG^G[AT]CCG
EagI 2623 CGGCCG C^GGCCG
EclXI 2623 CGGCCG C^GGCCG
Eco52I 2623 CGGCCG C^GGCCG
EcoNI 1210 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2283 GAATTC G^AATTC
FspI 3704 TGCGCA TGC^GCA
HaeII 4421 AGCGCC [GA]GCGC^[CT]
HindIII 2209 AAGCTT A^AGCTT
HpaI 7 GTTAAC GTT^AAC
MvnI 4240 CGCG CG^CG
NaeI 3953 GCCGGC GCC^GGC
NgoMIV 3953 GCCGGC G^CCGGC
PshAI 1570 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3358 AACGTT AA^CGTT
RcaI 3456 TCATGA T^CATGA
RsrII 3994 CGGACCG CG^G[AT]CCG
SacI 4450 GAGCTC GAGCT^C
SalI 2645 GTCGAC G^TCGAC
ScaI 3052 AGTACT AGT^ACT
SmaI 2345 CCCGGG CCC^GGG
SspBI 1977 TGTACA T^GTACA
StuI 566 AGGCCT AGG^CCT
XmaCI 2345 CCCGGG C^CCGGG
XmaI 2345 CCCGGG C^CCGGG
XmnI 2763 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2645 GTCGAC GT^[AC][GT]AC
3546 GTCTAC
AcyI 353 GACGCC G[GA]^CG[CT]C
3598 GACGCC
AocI 2431 CCTCAGG CC^T[ACGT]AGG
3267 CCTCAGG
AseI 3241 ATTAAT AT^TAAT
4775 ATTAAT
AsnI 3241 ATTAAT AT^TAAT
4775 ATTAAT
AspI 2776 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3417 GACATGGTC
BclI 408 TGATCA T^GATCA
3734 TGATCA
BglI 1724 GCCCCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3953 GCCGGCTGGGC
BsaHI 353 GACGCC G[GA]^CG[CT]C
3598 GACGCC
BspLU11I 1373 ACATGT A^CATGT
3824 ACATGT
BspMI 675 ACCTGC ACCTGC(4/8)
4873 ACCTGC
BsrFI 2621 ACCGGC [GA]^CCGG[CT]
3953 GCCGGC
BstAPI 2667 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3707 GCACTCTGTGC
Bsu36I 2431 CCTCAGG CC^T[ACGT]AGG
3267 CCTCAGG
Cfr10I 2621 ACCGGC [GA]^CCGG[CT]
3953 GCCGGC
DraIII 3708 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4255 CACTAAGTG
DrdI 3199 GACCCTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3521 GACTGACTGGTC
DsaI 2123 CCATGG C^C[GA][CT]GG
3163 CCATGG
Eco81I 2431 CCTCAGG CC^T[ACGT]AGG
3267 CCTCAGG
FbaI 408 TGATCA T^GATCA
3734 TGATCA
Hin1I 353 GACGCC G[GA]^CG[CT]C
3598 GACGCC
NcoI 2123 CCATGG C^CATGG
3163 CCATGG
PflFI 2776 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3417 GACATGGTC
PshBI 3241 ATTAAT AT^TAAT
4775 ATTAAT
PstI 4275 CTGCAG CTGCA^G
4830 CTGCAG
SpeI 991 ACTAGT A^CTAGT
3322 ACTAGT
SphI 2351 GCATGC GCATG^C
3716 GCATGC
SspI 2053 AATATT AAT^ATT
4375 AATATT
Tth111I 2776 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3417 GACATGGTC
XcmI 2255 CCATAGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3613 CCAGGTTCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanII BbrPI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaBI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII