##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AclI AflII Aor51HI AscI
AseI AsnI Asp718I BfrI BsaBI
BsiWI BsmBI BspDI BssHII BstEII
BstPI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI HpaI KpnI MamI MluI
NheI NotI NruI PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SgrAI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 204 GACGTC GACGT^C
AccI 6201 GTATAC GT^[AC][GT]AC
AccIII 3338 TCCGGA T^CCGGA
AhdI 3169 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2451 GGGCCC GGGCC^C
AspEI 3169 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 823 TGCGCA TGC^GCA
BcgI 3372 CGATGCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 3338 TCCGGA T^CCGGA
BsiEI 1157 CGACCG CG[GA][CT]^CG
BspEI 3338 TCCGGA T^CCGGA
BsrDI 6850 GCAATG GCAATG(2/0)
Bst1107I 6201 GTATAC GTA^TAC
BstZ17I 6201 GTATAC GTA^TAC
Eam1105I 3169 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1186 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 823 TGCGCA TGC^GCA
HincII 1142 GTCAAC GT[CT]^[GA]AC
HindII 1142 GTCAAC GT[CT]^[GA]AC
HindIII 1369 AAGCTT A^AGCTT
MfeI 4949 CAATTG C^AATTG
MroI 3338 TCCGGA T^CCGGA
MunI 4949 CAATTG C^AATTG
NaeI 1416 GCCGGC GCC^GGC
NdeI 6845 CATATG CA^TATG
NgoMIV 1416 GCCGGC G^CCGGC
PflFI 3586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 4044 GAAGAGC GCTCTTC(1/4)
SexAI 6088 ACCTGGT A^CC[AT]GGT
SnaBI 5348 TACGTA TAC^GTA
SspI 4767 AATATT AAT^ATT
Tth111I 3586 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5360 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AocI 1240 CCTCAGG CC^T[ACGT]AGG
3538 CCTCAGG
AvrII 3016 CCTAGG C^CTAGG
3781 CCTAGG
BalI 2071 TGGCCA TGG^CCA
2796 TGGCCA
BamHI 3293 GGATCC G^GATCC
6344 GGATCC
BbeI 116 GGCGCC GGCGC^C
6114 GGCGCC
BglI 1239 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3526 GCCAGGCGGGC
BglII 1980 AGATCT A^GATCT
4457 AGATCT
BlnI 3016 CCTAGG C^CTAGG
3781 CCTAGG
Bsp1407I 32 TGTACA T^GTACA
5157 TGTACA
BspHI 4385 TCATGA T^CATGA
5218 TCATGA
BsrBI 950 GAGCGG CCGCTC(-3/-3)
3344 GAGCGG
BsrGI 32 TGTACA T^GTACA
5157 TGTACA
BstBI 3631 TTCGAA TT^CGAA
5741 TTCGAA
Bsu36I 1240 CCTCAGG CC^T[ACGT]AGG
3538 CCTCAGG
Eco81I 1240 CCTCAGG CC^T[ACGT]AGG
3538 CCTCAGG
EcoT22I 5495 ATGCAT ATGCA^T
5507 ATGCAT
HaeII 116 GGCGCC [GA]GCGC^[CT]
6114 GGCGCC
KasI 116 GGCGCC G^GCGCC
6114 GGCGCC
KspI 209 CCGCGG CCGC^GG
2673 CCGCGG
MluNI 2071 TGGCCA TGG^CCA
2796 TGGCCA
MscI 2071 TGGCCA TGG^CCA
2796 TGGCCA
NarI 116 GGCGCC GG^CGCC
6114 GGCGCC
NcoI 487 CCATGG C^CATGG
4512 CCATGG
NsiI 5495 ATGCAT ATGCA^T
5507 ATGCAT
NspV 3631 TTCGAA TT^CGAA
5741 TTCGAA
PflMI 978 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3963 CCAGAGATTGG
PpuMI 558 AGGTCCT [GA]G^G[AT]CC[CT]
2290 AGGTCCC
PvuII 2137 CAGCTG CAG^CTG
3008 CAGCTG
RcaI 4385 TCATGA T^CATGA
5218 TCATGA
SacII 209 CCGCGG CCGC^GG
2673 CCGCGG
SfoI 116 GGCGCC GGC^GCC
6114 GGCGCC
SfuI 3631 TTCGAA TT^CGAA
5741 TTCGAA
SspBI 32 TGTACA T^GTACA
5157 TGTACA
TaqI 3632 TCGA T^CGA
5742 TCGA
TthHB8I 3632 TCGA T^CGA
5742 TCGA
Van91I 978 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3963 CCAGAGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AgeI AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvCI BbvI BciVI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PinAI PleI PmaCI PmlI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmaCI
XmaI XmnI