##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AseI AsnI Asp700I AviII BamHI
BbeI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 4240 GGTACC G^GTACC
AccI 3342 GTCTAC GT^[AC][GT]AC
AflII 4800 CTTAAG C^TTAAG
AflIII 3178 ACATGT A^C[GA][CT]GT
AhdI 4913 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4965 CCTCAGG CC^T[ACGT]AGG
Aor51HI 949 AGCGCT AGC^GCT
Asp718I 4240 GGTACC G^GTACC
AspEI 4913 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2070 CACGTG CAC^GTG
BbvCI 2453 CCTCAGC CCTCAGC(-5/-2)
BcgI 1174 GCAATGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 131 GTATCC GTATCC(6/5)
BclI 3833 TGATCA T^GATCA
BfrI 4800 CTTAAG C^TTAAG
BglII 2939 AGATCT A^GATCT
BsaAI 2070 CACGTG [CT]AC^GT[GA]
BsmBI 502 GAGACG CGTCTC(1/5)
BsmI 446 GCATTC GAATGC(1/-1)
BspLU11I 3178 ACATGT A^CATGT
BsrBI 2043 CCGCTC CCGCTC(-3/-3)
BstEII 426 GGTTACC G^GT[ACGT]ACC
BstPI 426 GGTTACC G^GT[ACGT]ACC
Bsu36I 4965 CCTCAGG CC^T[ACGT]AGG
Eam1105I 4913 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 949 AGCGCT AGC^GCT
Eco81I 4965 CCTCAGG CC^T[ACGT]AGG
EcoO65I 426 GGTTACC G^GT[ACGT]ACC
EcoRI 3653 GAATTC G^AATTC
FbaI 3833 TGATCA T^GATCA
KpnI 4240 GGTACC GGTAC^C
KspI 136 CCGCGG CCGC^GG
NdeI 3378 CATATG CA^TATG
PflFI 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2070 CACGTG CAC^GTG
PmlI 2070 CACGTG CAC^GTG
SacI 1058 GAGCTC GAGCT^C
SacII 136 CCGCGG CCGC^GG
SmaI 26 CCCGGG CCC^GGG
Tth111I 1968 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 26 CCCGGG C^CCGGG
XmaI 26 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 3404 GTGCAC G^TGCAC
5039 GTGCAC
ApaLI 3404 GTGCAC G^TGCAC
5039 GTGCAC
BbsI 2881 GAAGAC GAAGAC(2/6)
4574 GAAGAC
BglI 919 GCCGTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1666 GCCCAGTTGGC
BlpI 107 GCTGAGC GC^T[ACGT]AGC
2221 GCTGAGC
Bpu1102I 107 GCTGAGC GC^T[ACGT]AGC
2221 GCTGAGC
BpuAI 2881 GAAGAC GAAGAC(2/6)
4574 GAAGAC
BsrDI 3547 CATTGC GCAATG(2/0)
1174 GCAATG
BsrFI 112 GCCGGC [GA]^CCGG[CT]
1006 GCCGGC
BssSI 4752 CACGAG CACGAG(-5/-1)
798 CTCGTG
BstAPI 764 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1939 GCAACTGGTGC
CelII 107 GCTGAGC GC^T[ACGT]AGC
2221 GCTGAGC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
1006 GCCGGC
DraI 2167 TTTAAA TTT^AAA
3827 TTTAAA
DrdI 1038 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1460 GACGGATGAGTC
MaeII 505 ACGT A^CGT
2071 ACGT
NaeI 112 GCCGGC GCC^GGC
1006 GCCGGC
NgoMIV 112 GCCGGC G^CCGGC
1006 GCCGGC
PpuMI 1450 AGGACCT [GA]G^G[AT]CC[CT]
2688 GGGTCCT
TaqI 839 TCGA T^CGA
937 TCGA
TthHB8I 839 TCGA T^CGA
937 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbvI BcnI BfaI
BlnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII