##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AclI AcsI AflII ApoI AscI
Asp700I BciVI BclI BfrI BglII
BsaBI Bsp1407I BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CpoI DraI DrdI EarI
EcoO65I EcoRI EcoT22I FbaI FseI
HindIII HpaI Ksp632I MamI MfeI
MunI NdeI NheI NotI NsiI
NspV PacI PmeI Psp1406I RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 986 GACGTC GACGT^C
Acc65I 2450 GGTACC G^GTACC
AccI 1823 GTCTAC GT^[AC][GT]AC
AgeI 1624 ACCGGT A^CCGGT
Aor51HI 2139 AGCGCT AGC^GCT
AseI 2975 ATTAAT AT^TAAT
AsnI 2975 ATTAAT AT^TAAT
Asp718I 2450 GGTACC G^GTACC
AvrII 3045 CCTAGG C^CTAGG
BamHI 4396 GGATCC G^GATCC
BbrPI 2495 CACGTG CAC^GTG
BbvCI 3630 CCTCAGC CCTCAGC(-5/-2)
BlnI 3045 CCTAGG C^CTAGG
BsaAI 2495 CACGTG [CT]AC^GT[GA]
BsiWI 1247 CGTACG C^GTACG
BspDI 4807 ATCGAT AT^CGAT
BssSI 4371 CTCGTG CACGAG(-5/-1)
ClaI 4807 ATCGAT AT^CGAT
DraIII 1194 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2444 CGGCCG C^GGCCG
EclXI 2444 CGGCCG C^GGCCG
Eco47III 2139 AGCGCT AGC^GCT
Eco52I 2444 CGGCCG C^GGCCG
EcoRV 3291 GATATC GAT^ATC
KpnI 2450 GGTACC GGTAC^C
NcoI 58 CCATGG C^CATGG
NruI 3315 TCGCGA TCG^CGA
NspI 659 ACATGC [GA]CATG^[CT]
PaeR7I 2723 CTCGAG C^TCGAG
PinAI 1624 ACCGGT A^CCGGT
PmaCI 2495 CACGTG CAC^GTG
PmlI 2495 CACGTG CAC^GTG
PshAI 3082 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2975 ATTAAT AT^TAAT
PvuI 2929 CGATCG CGAT^CG
SfiI 4315 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 754 AATATT AAT^ATT
XhoI 2723 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 4221 TCCGGA T^CCGGA
4399 TCCGGA
AhdI 415 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
931 GACGCTTAGTC
Alw44I 1458 GTGCAC G^TGCAC
1836 GTGCAC
AlwNI 3182 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
4060 CAGCCTCTG
AocI 2660 CCTCAGG CC^T[ACGT]AGG
3748 CCTCAGG
ApaLI 1458 GTGCAC G^TGCAC
1836 GTGCAC
AspEI 415 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
931 GACGCTTAGTC
AspI 3538 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACTTGGTC
BlpI 1276 GCTTAGC GC^T[ACGT]AGC
4167 GCTGAGC
Bpu1102I 1276 GCTTAGC GC^T[ACGT]AGC
4167 GCTGAGC
BseAI 4221 TCCGGA T^CCGGA
4399 TCCGGA
BsiEI 2444 CGGCCG CG[GA][CT]^CG
2929 CGATCG
BspEI 4221 TCCGGA T^CCGGA
4399 TCCGGA
BsrBI 4247 CCGCTC CCGCTC(-3/-3)
1633 GAGCGG
BsrDI 1393 CATTGC GCAATG(2/0)
1579 GCAATG
BstAPI 1193 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1420 GCAGGGGGTGC
Bsu36I 2660 CCTCAGG CC^T[ACGT]AGG
3748 CCTCAGG
CelII 1276 GCTTAGC GC^T[ACGT]AGC
4167 GCTGAGC
Eam1105I 415 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
931 GACGCTTAGTC
Eco81I 2660 CCTCAGG CC^T[ACGT]AGG
3748 CCTCAGG
EcoNI 574 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2032 CCTGGCCAAGG
MroI 4221 TCCGGA T^CCGGA
4399 TCCGGA
PflFI 3538 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACTTGGTC
PvuII 1145 CAGCTG CAG^CTG
2270 CAGCTG
StuI 1347 AGGCCT AGG^CCT
2029 AGGCCT
Tth111I 3538 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4179 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbeI BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI