##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AcyI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI BamHI BbeI
BbrPI BcgI BlpI Bpu1102I BsaBI
BsaHI BsiWI BsmBI BspDI BspHI
BssHII BstBI BstEII BstPI Bsu36I
CelII ClaI CpoI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FauI FseI HaeII Hin1I KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SphI Sse8387I SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1090 CGCG CG^CG
AccIII 1823 TCCGGA T^CCGGA
AflIII 4330 ACATGT A^C[GA][CT]GT
AgeI 1146 ACCGGT A^CCGGT
AseI 4356 ATTAAT AT^TAAT
AsnI 4356 ATTAAT AT^TAAT
Asp700I 2256 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2803 TGCGCA TGC^GCA
BbsI 3016 GTCTTC GAAGAC(2/6)
BbvCI 1349 CCTCAGC CCTCAGC(-5/-2)
BciVI 3416 GGATAC GTATCC(6/5)
BglI 916 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 384 AGATCT A^GATCT
BpuAI 3016 GTCTTC GAAGAC(2/6)
BseAI 1823 TCCGGA T^CCGGA
BsgI 2301 CTGCAC GTGCAG(16/14)
BsiEI 2486 CGGCCG CG[GA][CT]^CG
BspEI 1823 TCCGGA T^CCGGA
BspLU11I 4330 ACATGT A^CATGT
BsrBI 1766 GAGCGG CCGCTC(-3/-3)
BsrDI 1958 GCAATG GCAATG(2/0)
BsrFI 1146 ACCGGT [GA]^CCGG[CT]
BssSI 4243 CTCGTG CACGAG(-5/-1)
Bst1107I 226 GTATAC GTA^TAC
BstUI 1090 CGCG CG^CG
BstXI 3161 CCAGCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 226 GTATAC GTA^TAC
Cfr10I 1146 ACCGGT [GA]^CCGG[CT]
EagI 2486 CGGCCG C^GGCCG
EclXI 2486 CGGCCG C^GGCCG
Eco52I 2486 CGGCCG C^GGCCG
EcoNI 84 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2803 TGCGCA TGC^GCA
HgaI 1235 GACGC GACGC(5/10)
HpaI 1410 GTTAAC GTT^AAC
MroI 1823 TCCGGA T^CCGGA
MvnI 1090 CGCG CG^CG
PinAI 1146 ACCGGT A^CCGGT
PshBI 4356 ATTAAT AT^TAAT
PstI 1282 CTGCAG CTGCA^G
SacI 2324 GAGCTC GAGCT^C
SalI 2787 GTCGAC G^TCGAC
ScaI 1665 AGTACT AGT^ACT
SexAI 3725 ACCTGGT A^CC[AT]GGT
SnaBI 1073 TACGTA TAC^GTA
SspI 1242 AATATT AAT^ATT
XmnI 2256 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1311 AAATTC [GA]^AATT[CT]
4136 GAATTT
AflII 718 CTTAAG C^TTAAG
1207 CTTAAG
ApoI 1311 AAATTC [GA]^AATT[CT]
4136 GAATTT
AvrII 2199 CCTAGG C^CTAGG
3393 CCTAGG
BalI 2999 TGGCCA TGG^CCA
3894 TGGCCA
BclI 985 TGATCA T^GATCA
2904 TGATCA
BfrI 718 CTTAAG C^TTAAG
1207 CTTAAG
BlnI 2199 CCTAGG C^CTAGG
3393 CCTAGG
BsaAI 1073 TACGTA [CT]AC^GT[GA]
4400 CACGTA
BstYI 384 AGATCT [GA]^GATC[CT]
3129 GGATCT
DraI 1081 TTTAAA TTT^AAA
2549 TTTAAA
EcoRV 461 GATATC GAT^ATC
1044 GATATC
FbaI 985 TGATCA T^GATCA
2904 TGATCA
MflI 384 AGATCT [GA]^GATC[CT]
3129 GGATCT
MluNI 2999 TGGCCA TGG^CCA
3894 TGGCCA
MscI 2999 TGGCCA TGG^CCA
3894 TGGCCA
NdeI 522 CATATG CA^TATG
2705 CATATG
NheI 145 GCTAGC G^CTAGC
1814 GCTAGC
PaeR7I 729 CTCGAG C^TCGAG
1942 CTCGAG
PflMI 641 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3690 CCAGGATGTGG
PpuMI 1227 AGGACCC [GA]G^G[AT]CC[CT]
1345 AGGACCT
SpeI 1388 ACTAGT A^CTAGT
2986 ACTAGT
Van91I 641 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3690 CCAGGATGTGG
XcmI 911 CCACTGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3716 CCAGTTCAAACCTGG
XhoI 729 CTCGAG C^TCGAG
1942 CTCGAG
XhoII 384 AGATCT [GA]^GATC[CT]
3129 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I