##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AvaII AviII
BbeI BbrPI BbvCI BfrI BglI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SexAI SfiI SfoI SgrAI
SmaI SnaBI StuI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 4569 GTAGAC GT^[AC][GT]AC
AlwNI 1931 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1216 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4059 CCTAGG C^CTAGG
BanI 987 GGTGCC G^G[CT][GA]CC
BanII 1195 GGGCTC G[GA]GC[CT]^C
BcgI 1017 GCAGCTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1326 GTATCC GTATCC(6/5)
BcnI 1542 CCCGG CC^[GC]GG
BglII 3208 AGATCT A^GATCT
BlnI 4059 CCTAGG C^CTAGG
BlpI 10 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC
BsaAI 1151 TACGTG [CT]AC^GT[GA]
BsaBI 751 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 793 GGTCTC GGTCTC(1/5)
BsmBI 61 GAGACG CGTCTC(1/5)
BspHI 2149 TCATGA T^CATGA
BstBI 1715 TTCGAA TT^CGAA
BstEII 1821 GGTTACC G^GT[ACGT]ACC
BstPI 1821 GGTTACC G^GT[ACGT]ACC
CelII 10 GCTCAGC GC^T[ACGT]AGC
DraII 4055 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 4055 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1821 GGTTACC G^GT[ACGT]ACC
EcoRI 906 GAATTC G^AATTC
HapII 1543 CCGG C^CGG
HgaI 47 GACGC GACGC(5/10)
HpaII 1543 CCGG C^CGG
MamI 751 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1568 CAATTG C^AATTG
MspI 1543 CCGG C^CGG
MunI 1568 CAATTG C^AATTG
NciI 1542 CCCGG CC^[GC]GG
NcoI 1646 CCATGG C^CATGG
NspV 1715 TTCGAA TT^CGAA
PflFI 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2149 TCATGA T^CATGA
SapI 1875 GCTCTTC GCTCTTC(1/4)
SbfI 4289 CCTGCAGG CCTGCA^GG
ScaI 1592 AGTACT AGT^ACT
SfuI 1715 TTCGAA TT^CGAA
Sse8387I 4289 CCTGCAGG CCTGCA^GG
SspI 2421 AATATT AAT^ATT
Tth111I 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 819 TCTAGA T^CTAGA
XcmI 1778 CCAATTTACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1216 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 3486 ACATGT A^C[GA][CT]GT
4276 ACATGT
BalI 1345 TGGCCA TGG^CCA
3453 TGGCCA
BamHI 4366 GGATCC G^GATCC
4458 GGATCC
BbsI 144 GTCTTC GAAGAC(2/6)
4671 GTCTTC
BpuAI 144 GTCTTC GAAGAC(2/6)
4671 GTCTTC
BsgI 920 CTGCAC GTGCAG(16/14)
1706 CTGCAC
BsmI 649 GCATTC GAATGC(1/-1)
2520 GCATTC
BspLU11I 3486 ACATGT A^CATGT
4276 ACATGT
CfoI 212 GCGC GCG^C
3844 GCGC
DrdI 613 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
885 GACAGAGCTGTC
DsaI 26 CCGTGG C^C[GA][CT]GG
1646 CCATGG
EaeI 1345 TGGCCA [CT]^GGCC[GA]
3453 TGGCCA
EcoNI 1330 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2198 CCTAAGGGAGG
EcoT22I 1635 ATGCAT ATGCA^T
3095 ATGCAT
FauI 319 GCGGG CCCGC(4/6)
1130 GCGGG
HhaI 212 GCGC GCG^C
3844 GCGC
HinP1I 212 GCGC G^CGC
3844 GCGC
HincII 1183 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
HindII 1183 GTCAAC GT[CT]^[GA]AC
2473 GTTGAC
HindIII 955 AAGCTT A^AGCTT
2858 AAGCTT
MluNI 1345 TGGCCA TGG^CCA
3453 TGGCCA
MscI 1345 TGGCCA TGG^CCA
3453 TGGCCA
NdeI 2525 CATATG CA^TATG
4025 CATATG
NsiI 1635 ATGCAT ATGCA^T
3095 ATGCAT
SpeI 799 ACTAGT A^CTAGT
4486 ACTAGT
SphI 4206 GCATGC GCATG^C
4300 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI BbvI
BclI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
EarI Eco81I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII