##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AccIII AflIII AgeI
AscI AseI AsnI AviII BbeI
BbrPI BbvCI BcgI BciVI BclI
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
EcoRV EcoT22I FbaI FseI FspI
KasI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SphI Sse8387I StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 1876 AACGTT AA^CGTT
AcyI 3302 GACGCC G[GA]^CG[CT]C
AflII 3038 CTTAAG C^TTAAG
AhdI 1782 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1849 CCTGAGG CC^T[ACGT]AGG
Aor51HI 268 AGCGCT AGC^GCT
Asp700I 1065 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1782 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3292 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2262 CCTAGG C^CTAGG
BalI 1084 TGGCCA TGG^CCA
BamHI 2667 GGATCC G^GATCC
BfrI 3038 CTTAAG C^TTAAG
BlnI 2262 CCTAGG C^CTAGG
BsaHI 3302 GACGCC G[GA]^CG[CT]C
BsaWI 117 TCCGGT [AT]^CCGG[AT]
BsmBI 1147 GAGACG CGTCTC(1/5)
Bsp1407I 898 TGTACA T^GTACA
BsrBI 258 GAGCGG CCGCTC(-3/-3)
BsrGI 898 TGTACA T^GTACA
BssHII 350 GCGCGC G^CGCGC
BssSI 617 CTCGTG CACGAG(-5/-1)
BstEII 3535 GGTCACC G^GT[ACGT]ACC
BstPI 3535 GGTCACC G^GT[ACGT]ACC
Bsu36I 1849 CCTGAGG CC^T[ACGT]AGG
DraIII 750 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3703 GACTGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1782 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 268 AGCGCT AGC^GCT
Eco81I 1849 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3535 GGTCACC G^GT[ACGT]ACC
Hin1I 3302 GACGCC G[GA]^CG[CT]C
HpaI 1879 GTTAAC GTT^AAC
MluNI 1084 TGGCCA TGG^CCA
MscI 1084 TGGCCA TGG^CCA
PflFI 3292 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 602 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1876 AACGTT AA^CGTT
PvuI 2626 CGATCG CGAT^CG
SapI 1463 GAAGAGC GCTCTTC(1/4)
SfiI 1227 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1036 CCCGGG CCC^GGG
SpeI 1067 ACTAGT A^CTAGT
SspBI 898 TGTACA T^GTACA
SspI 3072 AATATT AAT^ATT
Tth111I 3292 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3652 TCTAGA T^CTAGA
XmaCI 1036 CCCGGG C^CCGGG
XmaI 1036 CCCGGG C^CCGGG
XmnI 1065 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2708 GGTACC G^GTACC
2757 GGTACC
Alw44I 1773 GTGCAC G^TGCAC
3801 GTGCAC
ApaLI 1773 GTGCAC G^TGCAC
3801 GTGCAC
Asp718I 2708 GGTACC G^GTACC
2757 GGTACC
BglII 1312 AGATCT A^GATCT
3611 AGATCT
BsgI 625 CTGCAC GTGCAG(16/14)
1419 GTGCAG
BsmI 3478 GAATGC GAATGC(1/-1)
2578 GCATTC
BstXI 566 CCACAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2761 CCAAATAGTTGG
EcoNI 3259 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3660 CCTAGCCCAGG
EcoRI 939 GAATTC G^AATTC
1441 GAATTC
HincII 1818 GTTGAC GT[CT]^[GA]AC
1879 GTTAAC
HindII 1818 GTTGAC GT[CT]^[GA]AC
1879 GTTAAC
HindIII 3351 AAGCTT A^AGCTT
3427 AAGCTT
KpnI 2708 GGTACC GGTAC^C
2757 GGTACC
NaeI 362 GCCGGC GCC^GGC
1228 GCCGGC
NcoI 1618 CCATGG C^CATGG
2441 CCATGG
NgoMIV 362 GCCGGC G^CCGGC
1228 GCCGGC
NspI 771 GCATGT [GA]CATG^[CT]
3224 GCATGT
SmlI 3038 CTTAAG C^T[CT][GA]AG
3511 CTTGAG
XcmI 1310 CCAGATCTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2545 CCAATTAAGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII