##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccIII AclI AcsI AgeI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BglII
BlnI BsaAI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI Tth111I
Van91I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2063 GGTACC G^GTACC
AccI 308 GTCGAC GT^[AC][GT]AC
AflII 720 CTTAAG C^TTAAG
AhdI 423 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 294 CCTGAGG CC^T[ACGT]AGG
Asp718I 2063 GGTACC G^GTACC
AspEI 423 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1317 GAGCTC G[AT]GC[AT]^C
BamHI 1715 GGATCC G^GATCC
BcnI 773 CCGGG CC^[GC]GG
BfrI 720 CTTAAG C^TTAAG
BsaWI 1966 ACCGGA [AT]^CCGG[AT]
BsgI 440 GTGCAG GTGCAG(16/14)
BsiHKAI 1317 GAGCTC G[AT]GC[AT]^C
BspMI 1070 GCAGGT ACCTGC(4/8)
BsrBI 289 CCGCTC CCGCTC(-3/-3)
BstEII 1176 GGTGACC G^GT[ACGT]ACC
BstPI 1176 GGTGACC G^GT[ACGT]ACC
BstXI 1231 CCATAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 294 CCTGAGG CC^T[ACGT]AGG
DraIII 952 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 423 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 294 CCTGAGG CC^T[ACGT]AGG
EcoNI 1738 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1176 GGTGACC G^GT[ACGT]ACC
EcoT14I 1914 CCTTGG C^C[AT][AT]GG
HincII 308 GTCGAC GT[CT]^[GA]AC
HindII 308 GTCGAC GT[CT]^[GA]AC
KpnI 2063 GGTACC GGTAC^C
KspI 411 CCGCGG CCGC^GG
NciI 773 CCGGG CC^[GC]GG
NdeI 303 CATATG CA^TATG
PshAI 1350 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1317 GAGCTC GAGCT^C
SacII 411 CCGCGG CCGC^GG
SalI 308 GTCGAC G^TCGAC
SspI 886 AATATT AAT^ATT
StuI 227 AGGCCT AGG^CCT
StyI 1914 CCTTGG C^C[AT][AT]GG
SwaI 2502 ATTTAAAT ATTT^AAAT
XcmI 2189 CCAGCATTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 412 CGCG CG^CG
1599 CGCG
AcyI 1370 GGCGTC G[GA]^CG[CT]C
1605 GGCGTC
AflIII 565 ACATGT A^C[GA][CT]GT
2239 ACATGT
AvaI 266 CTCGAG C^[CT]CG[GA]G
1361 CTCGAG
BanI 1292 GGTGCC G^G[CT][GA]CC
2063 GGTACC
BanII 455 GAGCCC G[GA]GC[CT]^C
1317 GAGCTC
BbsI 86 GAAGAC GAAGAC(2/6)
329 GTCTTC
BglI 112 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
783 GCCCAGTTGGC
BlpI 7 GCTCAGC GC^T[ACGT]AGC
452 GCTGAGC
Bpu1102I 7 GCTCAGC GC^T[ACGT]AGC
452 GCTGAGC
BpuAI 86 GAAGAC GAAGAC(2/6)
329 GTCTTC
BsaHI 1370 GGCGTC G[GA]^CG[CT]C
1605 GGCGTC
BsaI 1488 GAGACC GGTCTC(1/5)
1990 GAGACC
BsoBI 266 CTCGAG C^[CT]CG[GA]G
1361 CTCGAG
BspLU11I 565 ACATGT A^CATGT
2239 ACATGT
BstUI 412 CGCG CG^CG
1599 CGCG
BstYI 1643 GGATCT [GA]^GATC[CT]
1715 GGATCC
CelII 7 GCTCAGC GC^T[ACGT]AGC
452 GCTGAGC
CfoI 5 GCGC GCG^C
683 GCGC
DpnII 1644 GATC ^GATC
1716 GATC
HaeII 4 GGCGCT [GA]GCGC^[CT]
682 AGCGCC
HapII 773 CCGG C^CGG
1967 CCGG
HgaI 1371 GCGTC GACGC(5/10)
1606 GCGTC
HhaI 5 GCGC GCG^C
683 GCGC
Hin1I 1370 GGCGTC G[GA]^CG[CT]C
1605 GGCGTC
HinP1I 5 GCGC G^CGC
683 GCGC
HpaII 773 CCGG C^CGG
1967 CCGG
MaeII 1213 ACGT A^CGT
1288 ACGT
MboI 1644 GATC ^GATC
1716 GATC
MflI 1643 GGATCT [GA]^GATC[CT]
1715 GGATCC
MspI 773 CCGG C^CGG
1967 CCGG
MvnI 412 CGCG CG^CG
1599 CGCG
NdeII 1644 GATC ^GATC
1716 GATC
PaeR7I 266 CTCGAG C^TCGAG
1361 CTCGAG
PstI 627 CTGCAG CTGCA^G
1335 CTGCAG
PvuII 450 CAGCTG CAG^CTG
729 CAGCTG
SapI 1010 GAAGAGC GCTCTTC(1/4)
1065 GAAGAGC
Sau3AI 1644 GATC ^GATC
1716 GATC
ScaI 1618 AGTACT AGT^ACT
1695 AGTACT
SexAI 239 ACCAGGT A^CC[AT]GGT
1993 ACCTGGT
SfaNI 99 GATGC GCATC(5/9)
1509 GATGC
SphI 739 GCATGC GCATG^C
2033 GCATGC
XhoI 266 CTCGAG C^TCGAG
1361 CTCGAG
XhoII 1643 GGATCT [GA]^GATC[CT]
1715 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraI DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MslI MspA1I MvaI MwoI NlaIII
NlaIV NspI PleI PpuMI RcaI
RsaI Sau96I ScrFI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I