##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BcgI BglII BsaBI BsiWI BspDI
BspHI BspLU11I BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
HindIII HpaI KpnI KspI MamI
MluI NotI NruI NsiI PacI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SgrAI SnaBI
SpeI SspI SwaI Van91I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 2460 GTCTAC GT^[AC][GT]AC
AccII 2445 CGCG CG^CG
AccIII 869 TCCGGA T^CCGGA
AcsI 1398 AAATTT [GA]^AATT[CT]
AflII 2739 CTTAAG C^TTAAG
AflIII 3558 ACACGT A^C[GA][CT]GT
ApoI 1398 AAATTT [GA]^AATT[CT]
AspI 1581 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2566 TGCGCA TGC^GCA
AvrII 3307 CCTAGG C^CTAGG
BamHI 155 GGATCC G^GATCC
BbeI 1213 GGCGCC GGCGC^C
BbsI 1470 GTCTTC GAAGAC(2/6)
BciVI 533 GGATAC GTATCC(6/5)
BclI 1996 TGATCA T^GATCA
BfrI 2739 CTTAAG C^TTAAG
BlnI 3307 CCTAGG C^CTAGG
BpuAI 1470 GTCTTC GAAGAC(2/6)
BsaI 2818 GAGACC GGTCTC(1/5)
BsaWI 869 TCCGGA [AT]^CCGG[AT]
BseAI 869 TCCGGA T^CCGGA
BsmBI 497 CGTCTC CGTCTC(1/5)
Bsp1407I 2621 TGTACA T^GTACA
BspEI 869 TCCGGA T^CCGGA
BsrDI 3264 GCAATG GCAATG(2/0)
BsrGI 2621 TGTACA T^GTACA
BssSI 2040 CACGAG CACGAG(-5/-1)
BstUI 2445 CGCG CG^CG
DraI 168 TTTAAA TTT^AAA
FbaI 1996 TGATCA T^GATCA
FseI 2334 GGCCGGCC GGCCGG^CC
FspI 2566 TGCGCA TGC^GCA
HincII 3352 GTTGAC GT[CT]^[GA]AC
HindII 3352 GTTGAC GT[CT]^[GA]AC
KasI 1213 GGCGCC G^GCGCC
MfeI 3331 CAATTG C^AATTG
MroI 869 TCCGGA T^CCGGA
MslI 922 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3331 CAATTG C^AATTG
MvnI 2445 CGCG CG^CG
NarI 1213 GGCGCC GG^CGCC
NdeI 2928 CATATG CA^TATG
NheI 2646 GCTAGC G^CTAGC
NspI 3750 GCATGC [GA]CATG^[CT]
PaeR7I 3573 CTCGAG C^TCGAG
PflFI 1581 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 1213 GGCGCC GGC^GCC
SphI 3750 GCATGC GCATG^C
SspBI 2621 TGTACA T^GTACA
Tth111I 1581 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3573 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 317 GGCGTC G[GA]^CG[CT]C
1213 GGCGCC
Alw44I 1352 GTGCAC G^TGCAC
3760 GTGCAC
AocI 159 CCTCAGG CC^T[ACGT]AGG
2547 CCTGAGG
Aor51HI 3202 AGCGCT AGC^GCT
3369 AGCGCT
ApaI 950 GGGCCC GGGCC^C
3090 GGGCCC
ApaLI 1352 GTGCAC G^TGCAC
3760 GTGCAC
Asp700I 1529 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1875 GAATGCTTTC
BlpI 1 GCTGAGC GC^T[ACGT]AGC
1229 GCTGAGC
Bpu1102I 1 GCTGAGC GC^T[ACGT]AGC
1229 GCTGAGC
BsaHI 317 GGCGTC G[GA]^CG[CT]C
1213 GGCGCC
BsmI 413 GAATGC GAATGC(1/-1)
1875 GAATGC
BstBI 1882 TTCGAA TT^CGAA
3707 TTCGAA
BstXI 574 CCAACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1099 CCAGCAACCTGG
Bsu36I 159 CCTCAGG CC^T[ACGT]AGG
2547 CCTGAGG
CelII 1 GCTGAGC GC^T[ACGT]AGC
1229 GCTGAGC
Eco47III 3202 AGCGCT AGC^GCT
3369 AGCGCT
Eco81I 159 CCTCAGG CC^T[ACGT]AGG
2547 CCTGAGG
Hin1I 317 GGCGTC G[GA]^CG[CT]C
1213 GGCGCC
NcoI 313 CCATGG C^CATGG
2632 CCATGG
NspV 1882 TTCGAA TT^CGAA
3707 TTCGAA
SacI 831 GAGCTC GAGCT^C
1803 GAGCTC
SapI 2093 GCTCTTC GCTCTTC(1/4)
3638 GCTCTTC
SbfI 2504 CCTGCAGG CCTGCA^GG
2822 CCTGCAGG
SfiI 1592 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3056 GGCCAAGGAGGCC
SfuI 1882 TTCGAA TT^CGAA
3707 TTCGAA
Sse8387I 2504 CCTGCAGG CCTGCA^GG
2822 CCTGCAGG
StuI 2044 AGGCCT AGG^CCT
2743 AGGCCT
TfiI 102 GATTC G^A[AT]TC
3630 GAATC
XmnI 1529 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1875 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI