##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbrPI
BciVI BglII BlnI BlpI Bpu1102I
BsiWI BsmI BssHII Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I CelII
DraIII Eam1105I Eco47III EcoRI EcoRV
FseI FspI HpaI KpnI MflI
MluI NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII SalI SbfI SexAI SfiI
SfuI SgrAI SphI Sse8387I StuI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 896 GTCTAC GT^[AC][GT]AC
Alw44I 4579 GTGCAC G^TGCAC
AlwNI 3383 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 4579 GTGCAC G^TGCAC
AspI 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1305 TGGCCA TGG^CCA
BbsI 2604 GAAGAC GAAGAC(2/6)
BbvCI 2165 CCTCAGC CCTCAGC(-5/-2)
BglI 554 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2604 GAAGAC GAAGAC(2/6)
BsaBI 4552 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 565 GAGACC GGTCTC(1/5)
BsiEI 557 CGGCCG CG[GA][CT]^CG
BsmBI 2720 GAGACG CGTCTC(1/5)
BspHI 1473 TCATGA T^CATGA
BspLU11I 3082 ACATGT A^CATGT
BspMI 2421 ACCTGC ACCTGC(4/8)
BstEII 1548 GGTCACC G^GT[ACGT]ACC
BstPI 1548 GGTCACC G^GT[ACGT]ACC
CpoI 31 CGGACCG CG^G[AT]CCG
DrdI 1540 GACATTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 557 CGGCCG C^GGCCG
EclXI 557 CGGCCG C^GGCCG
Eco52I 557 CGGCCG C^GGCCG
EcoO65I 1548 GGTCACC G^GT[ACGT]ACC
EcoT22I 3803 ATGCAT ATGCA^T
HindIII 2252 AAGCTT A^AGCTT
MamI 4552 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3329 CAATTG C^AATTG
MluNI 1305 TGGCCA TGG^CCA
MscI 1305 TGGCCA TGG^CCA
MunI 3329 CAATTG C^AATTG
NaeI 559 GCCGGC GCC^GGC
NgoMIV 559 GCCGGC G^CCGGC
NsiI 3803 ATGCAT ATGCA^T
PflFI 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4231 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1696 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 2135 CTGCAG CTGCA^G
RcaI 1473 TCATGA T^CATGA
RsrII 31 CGGACCG CG^G[AT]CCG
SacI 2352 GAGCTC GAGCT^C
SapI 2141 GCTCTTC GCTCTTC(1/4)
SnaBI 2071 TACGTA TAC^GTA
SwaI 3369 ATTTAAAT ATTT^AAAT
Tth111I 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4231 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3175 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 248 TCCGGA T^CCGGA
1512 TCCGGA
AflII 1078 CTTAAG C^TTAAG
3916 CTTAAG
AflIII 831 ACACGT A^C[GA][CT]GT
3082 ACATGT
BcgI 1941 CGAGATGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
335 GCAACCGCCTCG
BclI 1800 TGATCA T^GATCA
3797 TGATCA
BfrI 1078 CTTAAG C^TTAAG
3916 CTTAAG
BpmI 2324 CTCCAG CTGGAG(16/14)
2015 CTGGAG
BsaAI 2071 TACGTA [CT]AC^GT[GA]
3702 CACGTA
BseAI 248 TCCGGA T^CCGGA
1512 TCCGGA
BseRI 411 CTCCTC GAGGAG(10/8)
1882 GAGGAG
Bsp1407I 1038 TGTACA T^GTACA
3085 TGTACA
BspDI 1454 ATCGAT AT^CGAT
2066 ATCGAT
BspEI 248 TCCGGA T^CCGGA
1512 TCCGGA
BsrBI 487 CCGCTC CCGCTC(-3/-3)
631 GAGCGG
BsrGI 1038 TGTACA T^GTACA
3085 TGTACA
BssSI 1565 CACGAG CACGAG(-5/-1)
2740 CTCGTG
ClaI 1454 ATCGAT AT^CGAT
2066 ATCGAT
EcoNI 946 CCTAATATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1957 CCTCTTGTAGG
FbaI 1800 TGATCA T^GATCA
3797 TGATCA
HincII 2475 GTCAAC GT[CT]^[GA]AC
4189 GTTGAC
HindII 2475 GTCAAC GT[CT]^[GA]AC
4189 GTTGAC
MroI 248 TCCGGA T^CCGGA
1512 TCCGGA
NdeI 2042 CATATG CA^TATG
4235 CATATG
NspI 2457 ACATGC [GA]CATG^[CT]
3082 ACATGT
PleI 2322 GACTC GAGTC(4/5)
1254 GAGTC
ScaI 2314 AGTACT AGT^ACT
3912 AGTACT
SmaI 84 CCCGGG CCC^GGG
536 CCCGGG
SpeI 2611 ACTAGT A^CTAGT
4135 ACTAGT
SspBI 1038 TGTACA T^GTACA
3085 TGTACA
XcmI 812 CCAAAGTCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2609 CCACTAGTTCAATGG
XmaCI 84 CCCGGG C^CCGGG
536 CCCGGG
XmaI 84 CCCGGG C^CCGGG
536 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BmyI BsaHI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI