##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglII
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FbaI
FseI FspI HapII Hin1I HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 437 GTAGAC GT^[AC][GT]AC
AflIII 1474 ACATGT A^C[GA][CT]GT
AhdI 872 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 474 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 497 GGGCCC GGGCC^C
AspEI 872 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 12 GTGCTC G[AT]GC[AT]^C
AvrII 1669 CCTAGG C^CTAGG
BglI 1295 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1669 CCTAGG C^CTAGG
BsaAI 39 CACGTA [CT]AC^GT[GA]
BsaI 778 GGTCTC GGTCTC(1/5)
BsiHKAI 12 GTGCTC G[AT]GC[AT]^C
BspLU11I 1474 ACATGT A^CATGT
BsrDI 222 GCAATG GCAATG(2/0)
BstAPI 1909 GCACTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1092 TTCGAA TT^CGAA
BstXI 1192 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 969 GGATCT [GA]^GATC[CT]
DraI 2442 TTTAAA TTT^AAA
DraII 1540 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1910 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2643 CCATGG C^C[GA][CT]GG
Eam1105I 872 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1540 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2451 GAATTC G^AATTC
EcoT22I 2268 ATGCAT ATGCA^T
FauI 495 GCGGG CCCGC(4/6)
HgaI 1381 GCGTC GACGC(5/10)
HincII 1383 GTCAAC GT[CT]^[GA]AC
HindII 1383 GTCAAC GT[CT]^[GA]AC
MaeII 40 ACGT A^CGT
MflI 969 GGATCT [GA]^GATC[CT]
NcoI 2643 CCATGG C^CATGG
NsiI 2268 ATGCAT ATGCA^T
NspV 1092 TTCGAA TT^CGAA
PpuMI 1540 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 800 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1092 TTCGAA TT^CGAA
SpeI 2284 ACTAGT A^CTAGT
SphI 34 GCATGC GCATG^C
StuI 958 AGGCCT AGG^CCT
XbaI 2661 TCTAGA T^CTAGA
XhoII 969 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 898 GATCC GGATC(4/5)
969 GGATC
BanII 497 GGGCCC G[GA]GC[CT]^C
2498 GAGCCC
BbsI 331 GAAGAC GAAGAC(2/6)
1166 GAAGAC
BbvCI 1039 CCTCAGC CCTCAGC(-5/-2)
80 GCTGAGG
BpuAI 331 GAAGAC GAAGAC(2/6)
1166 GAAGAC
BsiYI 485 CCCAATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1634 CCTAGATTTGG
BslI 485 CCCAATGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1634 CCTAGATTTGG
BspHI 1242 TCATGA T^CATGA
1440 TCATGA
EcoRV 278 GATATC GAT^ATC
1055 GATATC
HaeII 363 GGCGCT [GA]GCGC^[CT]
1401 AGCGCC
NspI 34 GCATGC [GA]CATG^[CT]
1474 ACATGT
PstI 432 CTGCAG CTGCA^G
1781 CTGCAG
PvuII 116 CAGCTG CAG^CTG
608 CAGCTG
RcaI 1242 TCATGA T^CATGA
1440 TCATGA
SspI 2579 AATATT AAT^ATT
2782 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BbvI BfaI BmyI
BpmI BsaJI BseRI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI