##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I ApaLI AscI AspEI AspHI
BbeI BbrPI BbvCI BcgI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspEI BsrGI BssHII BstBI BstEII
BstPI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
KasI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SexAI SfiI SfoI
SfuI SmaI SpeI SphI SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 4358 CTTAAG C^TTAAG
AocI 5001 CCTAAGG CC^T[ACGT]AGG
Aor51HI 5404 AGCGCT AGC^GCT
ApaI 149 GGGCCC GGGCC^C
Asp700I 5431 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3397 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1695 CTCGGG C^[CT]CG[GA]G
AviII 5121 TGCGCA TGC^GCA
BciVI 4383 GTATCC GTATCC(6/5)
BclI 2833 TGATCA T^GATCA
BfrI 4358 CTTAAG C^TTAAG
BglII 213 AGATCT A^GATCT
BlpI 194 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 194 GCTGAGC GC^T[ACGT]AGC
BsaAI 5086 TACGTA [CT]AC^GT[GA]
BsaBI 1928 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2420 GGTCTC GGTCTC(1/5)
BsaWI 574 TCCGGT [AT]^CCGG[AT]
BsoBI 1695 CTCGGG C^[CT]CG[GA]G
BspHI 4789 TCATGA T^CATGA
BsrBI 43 GAGCGG CCGCTC(-3/-3)
BsrFI 2871 ACCGGC [GA]^CCGG[CT]
BssSI 2034 CTCGTG CACGAG(-5/-1)
Bst1107I 2312 GTATAC GTA^TAC
BstZ17I 2312 GTATAC GTA^TAC
Bsu36I 5001 CCTAAGG CC^T[ACGT]AGG
CelII 194 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2871 ACCGGC [GA]^CCGG[CT]
Eco47III 5404 AGCGCT AGC^GCT
Eco81I 5001 CCTAAGG CC^T[ACGT]AGG
EcoNI 3168 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2833 TGATCA T^GATCA
FspI 5121 TGCGCA TGC^GCA
HpaI 2101 GTTAAC GTT^AAC
KspI 153 CCGCGG CCGC^GG
MamI 1928 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3397 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1693 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 629 CAGCTG CAG^CTG
RcaI 4789 TCATGA T^CATGA
SacII 153 CCGCGG CCGC^GG
SalI 548 GTCGAC G^TCGAC
SbfI 2617 CCTGCAGG CCTGCA^GG
SgrAI 2870 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 5086 TACGTA TAC^GTA
Sse8387I 2617 CCTGCAGG CCTGCA^GG
Tth111I 3397 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5186 TCTAGA T^CTAGA
XmnI 5431 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 588 GACGCC G[GA]^CG[CT]C
3397 GACGCC
AseI 5465 ATTAAT AT^TAAT
5546 ATTAAT
AsnI 5465 ATTAAT AT^TAAT
5546 ATTAAT
AvrII 3377 CCTAGG C^CTAGG
4919 CCTAGG
BamHI 2066 GGATCC G^GATCC
2992 GGATCC
BanII 149 GGGCCC G[GA]GC[CT]^C
1676 GAGCCC
BglI 591 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1007 GCCACAGTGGC
BlnI 3377 CCTAGG C^CTAGG
4919 CCTAGG
BsaHI 588 GACGCC G[GA]^CG[CT]C
3397 GACGCC
BseRI 4530 CTCCTC GAGGAG(10/8)
4542 CTCCTC
BsgI 5154 CTGCAC GTGCAG(16/14)
1879 GTGCAG
BspDI 1598 ATCGAT AT^CGAT
3004 ATCGAT
ClaI 1598 ATCGAT AT^CGAT
3004 ATCGAT
DraI 5355 TTTAAA TTT^AAA
5704 TTTAAA
DraII 1254 GGGTCCT [GA]G^G[ACGT]CC[CT]
3015 AGGTCCC
DrdI 1901 GACAAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2429 GACAGCCCTGTC
EcoO109I 1254 GGGTCCT [GA]G^G[ACGT]CC[CT]
3015 AGGTCCC
EcoRI 2162 GAATTC G^AATTC
5529 GAATTC
EcoT22I 867 ATGCAT ATGCA^T
3882 ATGCAT
HaeII 135 AGCGCC [GA]GCGC^[CT]
5404 AGCGCT
Hin1I 588 GACGCC G[GA]^CG[CT]C
3397 GACGCC
HindIII 1581 AAGCTT A^AGCTT
2819 AAGCTT
NsiI 867 ATGCAT ATGCA^T
3882 ATGCAT
PflMI 1544 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2510 CCACCCAATGG
PpuMI 1254 GGGTCCT [GA]G^G[AT]CC[CT]
3015 AGGTCCC
PshBI 5465 ATTAAT AT^TAAT
5546 ATTAAT
SapI 2629 GAAGAGC GCTCTTC(1/4)
2573 GCTCTTC
StuI 1547 AGGCCT AGG^CCT
3486 AGGCCT
Van91I 1544 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2510 CCACCCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp718I AvaII BalI BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII