##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BspDI BsrBI BssHII BstBI BstUI
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI PvuI RsrII SacI
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI StuI SwaI TaqI
TthHB8I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 1451 TCCGGA T^CCGGA
AclI 1668 AACGTT AA^CGTT
AcyI 1711 GGCGCC G[GA]^CG[CT]C
AflIII 1411 ACATGT A^C[GA][CT]GT
AspI 2492 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2839 TGGCCA TGG^CCA
BbeI 1711 GGCGCC GGCGC^C
BbrPI 615 CACGTG CAC^GTG
BbvCI 1740 CCTCAGC CCTCAGC(-5/-2)
BciVI 1454 GGATAC GTATCC(6/5)
BglII 2754 AGATCT A^GATCT
BsaAI 615 CACGTG [CT]AC^GT[GA]
BsaHI 1711 GGCGCC G[GA]^CG[CT]C
BsaWI 1451 TCCGGA [AT]^CCGG[AT]
BseAI 1451 TCCGGA T^CCGGA
BsmBI 800 CGTCTC CGTCTC(1/5)
BsmI 1547 GAATGC GAATGC(1/-1)
Bsp1407I 2234 TGTACA T^GTACA
BspEI 1451 TCCGGA T^CCGGA
BspLU11I 1411 ACATGT A^CATGT
BsrGI 2234 TGTACA T^GTACA
DrdI 792 GACTATTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 1711 GGCGCC G[GA]^CG[CT]C
HincII 717 GTCAAC GT[CT]^[GA]AC
HindII 717 GTCAAC GT[CT]^[GA]AC
KasI 1711 GGCGCC G^GCGCC
MluNI 2839 TGGCCA TGG^CCA
MroI 1451 TCCGGA T^CCGGA
MscI 2839 TGGCCA TGG^CCA
NarI 1711 GGCGCC GG^CGCC
NheI 2718 GCTAGC G^CTAGC
PflFI 2492 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2294 GAGTC GAGTC(4/5)
PmaCI 615 CACGTG CAC^GTG
PmlI 615 CACGTG CAC^GTG
Psp1406I 1668 AACGTT AA^CGTT
SapI 2477 GCTCTTC GCTCTTC(1/4)
SbfI 2796 CCTGCAGG CCTGCA^GG
ScaI 240 AGTACT AGT^ACT
SexAI 2846 ACCAGGT A^CC[AT]GGT
SfoI 1711 GGCGCC GGC^GCC
SmaI 2041 CCCGGG CCC^GGG
SmlI 735 CTCAAG C^T[CT][GA]AG
SphI 983 GCATGC GCATG^C
Sse8387I 2796 CCTGCAGG CCTGCA^GG
SspBI 2234 TGTACA T^GTACA
SspI 315 AATATT AAT^ATT
TfiI 2346 GAATC G^A[AT]TC
Tth111I 2492 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2041 CCCGGG C^CCGGG
XmaI 2041 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 2954 AAATTC [GA]^AATT[CT]
3101 AAATTT
AlwNI 2324 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2791 CAGAGCCTG
AocI 762 CCTGAGG CC^T[ACGT]AGG
834 CCTGAGG
ApoI 2954 AAATTC [GA]^AATT[CT]
3101 AAATTT
BamHI 21 GGATCC G^GATCC
1208 GGATCC
BanI 1385 GGTGCC G^G[CT][GA]CC
1711 GGCGCC
BbsI 78 GAAGAC GAAGAC(2/6)
556 GTCTTC
BglI 1641 GCCGTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1721 GCCCCTGCGGC
BpuAI 78 GAAGAC GAAGAC(2/6)
556 GTCTTC
BsgI 2197 CTGCAC GTGCAG(16/14)
2429 CTGCAC
BsrFI 1220 GCCGGT [GA]^CCGG[CT]
1833 GCCGGT
BssSI 1821 CACGAG CACGAG(-5/-1)
1606 CTCGTG
Bst1107I 875 GTATAC GTA^TAC
2161 GTATAC
BstAPI 1967 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2790 GCAGAGCCTGC
BstEII 2803 GGTGACC G^GT[ACGT]ACC
2850 GGTGACC
BstPI 2803 GGTGACC G^GT[ACGT]ACC
2850 GGTGACC
BstZ17I 875 GTATAC GTA^TAC
2161 GTATAC
Bsu36I 762 CCTGAGG CC^T[ACGT]AGG
834 CCTGAGG
Cfr10I 1220 GCCGGT [GA]^CCGG[CT]
1833 GCCGGT
EaeI 1515 CGGCCA [CT]^GGCC[GA]
2839 TGGCCA
EarI 933 CTCTTC CTCTTC(1/4)
2478 CTCTTC
Eco81I 762 CCTGAGG CC^T[ACGT]AGG
834 CCTGAGG
EcoNI 1104 CCTATGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2581 CCTCTGGAAGG
EcoO65I 2803 GGTGACC G^GT[ACGT]ACC
2850 GGTGACC
HgaI 2285 GACGC GACGC(5/10)
382 GCGTC
HinfI 2294 GAGTC G^A[ACGT]TC
2346 GAATC
Ksp632I 933 CTCTTC CTCTTC(1/4)
2478 CTCTTC
NcoI 2695 CCATGG C^CATGG
3079 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanII
BbvI BcgI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII