##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I ApaLI AscI Asp718I AviII
BfrI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI EcoRV
EcoT22I FseI FspI HindIII KpnI
MamI MluI MroI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 4347 ACACGT A^C[GA][CT]GT
AgeI 337 ACCGGT A^CCGGT
AhdI 940 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1202 AGCGCT AGC^GCT
AseI 4543 ATTAAT AT^TAAT
AsnI 4543 ATTAAT AT^TAAT
AspEI 940 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 123 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1045 CACGTG CAC^GTG
BcgI 1664 GCAGCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4 AGATCT A^GATCT
BsmI 4335 GAATGC GAATGC(1/-1)
BsrDI 2318 GCAATG GCAATG(2/0)
BssHII 1494 GCGCGC G^CGCGC
BssSI 4071 CACGAG CACGAG(-5/-1)
BstAPI 1346 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 211 GGTTACC G^GT[ACGT]ACC
BstPI 211 GGTTACC G^GT[ACGT]ACC
DraI 4627 TTTAAA TTT^AAA
DrdI 3676 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 940 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1202 AGCGCT AGC^GCT
EcoO65I 211 GGTTACC G^GT[ACGT]ACC
EcoRI 4743 GAATTC G^AATTC
HpaI 3382 GTTAAC GTT^AAC
MslI 3814 CATGAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 2438 CTCGAG C^TCGAG
PflFI 123 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 337 ACCGGT A^CCGGT
PmaCI 1045 CACGTG CAC^GTG
PmlI 1045 CACGTG CAC^GTG
PshAI 367 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4543 ATTAAT AT^TAAT
ScaI 607 AGTACT AGT^ACT
Tth111I 123 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2147 CCAGGCCACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2438 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 977 GACGTC GACGT^C
1840 GACGTC
AocI 2479 CCTGAGG CC^T[ACGT]AGG
4472 CCTCAGG
AvrII 90 CCTAGG C^CTAGG
672 CCTAGG
BciVI 4482 GTATCC GTATCC(6/5)
4516 GTATCC
BclI 3614 TGATCA T^GATCA
4421 TGATCA
BlnI 90 CCTAGG C^CTAGG
672 CCTAGG
BsaAI 1045 CACGTG [CT]AC^GT[GA]
4348 CACGTA
BsaWI 337 ACCGGT [AT]^CCGG[AT]
923 ACCGGA
BsmBI 594 CGTCTC CGTCTC(1/5)
1327 CGTCTC
BspHI 2652 TCATGA T^CATGA
3002 TCATGA
BstXI 1777 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3018 CCAACTTGCTGG
Bsu36I 2479 CCTGAGG CC^T[ACGT]AGG
4472 CCTCAGG
EagI 774 CGGCCG C^GGCCG
1814 CGGCCG
EclXI 774 CGGCCG C^GGCCG
1814 CGGCCG
Eco52I 774 CGGCCG C^GGCCG
1814 CGGCCG
Eco81I 2479 CCTGAGG CC^T[ACGT]AGG
4472 CCTCAGG
FbaI 3614 TGATCA T^GATCA
4421 TGATCA
KspI 319 CCGCGG CCGC^GG
1526 CCGCGG
MfeI 2210 CAATTG C^AATTG
2885 CAATTG
MunI 2210 CAATTG C^AATTG
2885 CAATTG
NspI 2903 ACATGC [GA]CATG^[CT]
3374 GCATGT
PstI 1461 CTGCAG CTGCA^G
1591 CTGCAG
RcaI 2652 TCATGA T^CATGA
3002 TCATGA
SacII 319 CCGCGG CCGC^GG
1526 CCGCGG
SgrAI 1174 CGCCGGCG C[GA]^CCGG[CT]G
1674 CGCCGGTG
SmlI 1566 CTTGAG C^T[CT][GA]AG
2438 CTCGAG
StuI 87 AGGCCT AGG^CCT
1349 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI
XmnI