##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AcyI AgeI
Aor51HI ApaI AscI Asp718I AspI
AviII AvrII BalI BamHI BanI
BbeI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PflMI PinAI PmeI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 612 CGCG CG^CG
AccIII 538 TCCGGA T^CCGGA
AflII 1822 CTTAAG C^TTAAG
Alw44I 836 GTGCAC G^TGCAC
AocI 258 CCTGAGG CC^T[ACGT]AGG
ApaLI 836 GTGCAC G^TGCAC
AspHI 836 GTGCAC G[AT]GC[AT]^C
BanII 1704 GGGCTC G[GA]GC[CT]^C
BbrPI 966 CACGTG CAC^GTG
BbsI 1648 GTCTTC GAAGAC(2/6)
BbvCI 3 CCTCAGC CCTCAGC(-5/-2)
BfrI 1822 CTTAAG C^TTAAG
BglI 948 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1648 GTCTTC GAAGAC(2/6)
BsaBI 1888 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 399 GAGACC GGTCTC(1/5)
BsaWI 538 TCCGGA [AT]^CCGG[AT]
BseAI 538 TCCGGA T^CCGGA
BsiHKAI 836 GTGCAC G[AT]GC[AT]^C
Bsp1407I 889 TGTACA T^GTACA
BspDI 729 ATCGAT AT^CGAT
BspEI 538 TCCGGA T^CCGGA
BspHI 472 TCATGA T^CATGA
BspLU11I 886 ACATGT A^CATGT
BspMI 251 GCAGGT ACCTGC(4/8)
BsrBI 60 CCGCTC CCGCTC(-3/-3)
BsrGI 889 TGTACA T^GTACA
BstUI 612 CGCG CG^CG
Bsu36I 258 CCTGAGG CC^T[ACGT]AGG
CfoI 527 GCGC GCG^C
ClaI 729 ATCGAT AT^CGAT
DraIII 1236 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 341 GACCTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 222 CGGCCA [CT]^GGCC[GA]
Eco81I 258 CCTGAGG CC^T[ACGT]AGG
EcoNI 788 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1351 GAATTC G^AATTC
EcoT22I 1798 ATGCAT ATGCA^T
HaeII 526 GGCGCT [GA]GCGC^[CT]
HhaI 527 GCGC GCG^C
HinP1I 527 GCGC G^CGC
HindIII 935 AAGCTT A^AGCTT
MamI 1888 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 538 TCCGGA T^CCGGA
MvnI 612 CGCG CG^CG
NsiI 1798 ATGCAT ATGCA^T
PacI 2680 TTAATTAA TTAAT^TAA
PmaCI 966 CACGTG CAC^GTG
PmlI 966 CACGTG CAC^GTG
RcaI 472 TCATGA T^CATGA
ScaI 499 AGTACT AGT^ACT
SmaI 390 CCCGGG CCC^GGG
SmlI 1822 CTTAAG C^T[CT][GA]AG
SspBI 889 TGTACA T^GTACA
StuI 262 AGGCCT AGG^CCT
XbaI 1724 TCTAGA T^CTAGA
XmaCI 390 CCCGGG C^CCGGG
XmaI 390 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AclI 1758 AACGTT AA^CGTT
2675 AACGTT
AflIII 886 ACATGT A^C[GA][CT]GT
967 ACGTGT
AhdI 1284 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2265 GACTGAAAGTC
AlwNI 193 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1014 CAGACTCTG
Asp700I 905 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAAACGTTC
AspEI 1284 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2265 GACTGAAAGTC
AvaII 254 GGTCC G^G[AT]CC
1390 GGTCC
BmyI 836 GTGCAC G[AGT]GC[ACT]^C
1704 GGGCTC
BsaAI 550 TACGTG [CT]AC^GT[GA]
966 CACGTG
BsmI 1796 GAATGC GAATGC(1/-1)
145 GCATTC
Bsp1286I 836 GTGCAC G[AGT]GC[ACT]^C
1704 GGGCTC
BstYI 215 AGATCC [GA]^GATC[CT]
565 AGATCC
Eam1105I 1284 GACTGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2265 GACTGAAAGTC
FauI 59 CCCGC CCCGC(4/6)
961 CCCGC
MflI 215 AGATCC [GA]^GATC[CT]
565 AGATCC
PpuMI 253 AGGTCCC [GA]G^G[AT]CC[CT]
1389 AGGTCCT
Psp1406I 1758 AACGTT AA^CGTT
2675 AACGTT
XhoII 215 AGATCC [GA]^GATC[CT]
565 AGATCC
XmnI 905 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1755 GAAAACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaI BbvI
BcnI BfaI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I