##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AhdI AlwNI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BciVI BfrI BglII BlnI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspLU11I BsrDI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
MamI MfeI MluI MroI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 950 GGTACC G^GTACC
AflIII 1531 ACGTGT A^C[GA][CT]GT
AgeI 1975 ACCGGT A^CCGGT
AocI 482 CCTGAGG CC^T[ACGT]AGG
Asp718I 950 GGTACC G^GTACC
BalI 1113 TGGCCA TGG^CCA
BamHI 1346 GGATCC G^GATCC
BbrPI 1530 CACGTG CAC^GTG
BbsI 1437 GTCTTC GAAGAC(2/6)
BbvCI 1367 GCTGAGG CCTCAGC(-5/-2)
BlpI 1059 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1059 GCTGAGC GC^T[ACGT]AGC
BpuAI 1437 GTCTTC GAAGAC(2/6)
BsaAI 1530 CACGTG [CT]AC^GT[GA]
Bsp1407I 1325 TGTACA T^GTACA
BspHI 1453 TCATGA T^CATGA
BsrGI 1325 TGTACA T^GTACA
BssHII 2364 GCGCGC G^CGCGC
Bsu36I 482 CCTGAGG CC^T[ACGT]AGG
CelII 1059 GCTGAGC GC^T[ACGT]AGC
DrdI 1179 GACTCCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 477 CTCTTC CTCTTC(1/4)
Eco81I 482 CCTGAGG CC^T[ACGT]AGG
EcoNI 161 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KpnI 950 GGTACC GGTAC^C
Ksp632I 477 CTCTTC CTCTTC(1/4)
MluNI 1113 TGGCCA TGG^CCA
MscI 1113 TGGCCA TGG^CCA
MseI 2899 TTAA T^TAA
NcoI 1663 CCATGG C^CATGG
NspI 1144 GCATGT [GA]CATG^[CT]
PaeR7I 2265 CTCGAG C^TCGAG
PflMI 1376 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1975 ACCGGT A^CCGGT
PmaCI 1530 CACGTG CAC^GTG
PmlI 1530 CACGTG CAC^GTG
PshAI 827 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2436 CTGCAG CTGCA^G
PvuII 103 CAGCTG CAG^CTG
RcaI 1453 TCATGA T^CATGA
SacI 1083 GAGCTC GAGCT^C
SapI 476 GCTCTTC GCTCTTC(1/4)
SspBI 1325 TGTACA T^GTACA
StuI 1125 AGGCCT AGG^CCT
TfiI 1050 GATTC G^A[AT]TC
Tru9I 2899 TTAA T^TAA
Tsp509I 2897 AATT ^AATT
Van91I 1376 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2265 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1188 GTCTAC GT^[AC][GT]AC
1275 GTCTAC
Alw44I 63 GTGCAC G^TGCAC
780 GTGCAC
Aor51HI 1295 AGCGCT AGC^GCT
1894 AGCGCT
ApaLI 63 GTGCAC G^TGCAC
780 GTGCAC
BcgI 119 CGACCTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1093 CGAACTCCGTGC
BsaI 606 GAGACC GGTCTC(1/5)
2029 GAGACC
BsrBI 754 GAGCGG CCGCTC(-3/-3)
2104 GAGCGG
BssSI 2450 CACGAG CACGAG(-5/-1)
1071 CTCGTG
BstEII 762 GGTGACC G^GT[ACGT]ACC
3020 GGTGACC
BstPI 762 GGTGACC G^GT[ACGT]ACC
3020 GGTGACC
BstXI 1657 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2195 CCAAGACATTGG
DraIII 783 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2075 CACCGAGTG
EagI 1878 CGGCCG C^GGCCG
2014 CGGCCG
EclXI 1878 CGGCCG C^GGCCG
2014 CGGCCG
Eco47III 1295 AGCGCT AGC^GCT
1894 AGCGCT
Eco52I 1878 CGGCCG C^GGCCG
2014 CGGCCG
EcoO65I 762 GGTGACC G^GT[ACGT]ACC
3020 GGTGACC
MaeII 1531 ACGT A^CGT
1709 ACGT
SexAI 1037 ACCAGGT A^CC[AT]GGT
1150 ACCAGGT
SfaNI 2084 GATGC GCATC(5/9)
1680 GCATC
SfiI 1580 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1661 GGCCATGGAGGCC
SmlI 872 CTTGAG C^T[CT][GA]AG
2265 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KspI MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfcI SfoI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI