##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII Aor51HI AscI AseI
AsnI BbrPI BciVI BfrI BlpI
Bpu1102I BsaBI Bsp1407I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HindIII
HpaI KspI MamI MfeI MluI
MunI NheI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 677 GACGTC GACGT^C
Acc65I 1839 GGTACC G^GTACC
AccIII 1788 TCCGGA T^CCGGA
AgeI 1707 ACCGGT A^CCGGT
AhdI 3633 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1839 GGTACC G^GTACC
AspEI 3633 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3827 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1543 CCTAGG C^CTAGG
BcgI 975 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1473 TGATCA T^GATCA
BglII 132 AGATCT A^GATCT
BlnI 1543 CCTAGG C^CTAGG
BseAI 1788 TCCGGA T^CCGGA
BsiEI 2947 CGACCG CG[GA][CT]^CG
BsiWI 3766 CGTACG C^GTACG
BspDI 2109 ATCGAT AT^CGAT
BspEI 1788 TCCGGA T^CCGGA
BsrBI 1781 GAGCGG CCGCTC(-3/-3)
BsrDI 2576 CATTGC GCAATG(2/0)
BstAPI 433 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2109 ATCGAT AT^CGAT
Eam1105I 3633 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1859 ATGCAT ATGCA^T
FbaI 1473 TGATCA T^GATCA
HincII 3824 GTCGAC GT[CT]^[GA]AC
HindII 3824 GTCGAC GT[CT]^[GA]AC
KpnI 1839 GGTACC GGTAC^C
MroI 1788 TCCGGA T^CCGGA
NaeI 3298 GCCGGC GCC^GGC
NgoMIV 3298 GCCGGC G^CCGGC
NsiI 1859 ATGCAT ATGCA^T
PflFI 3827 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1707 ACCGGT A^CCGGT
PshAI 99 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3824 GTCGAC G^TCGAC
ScaI 669 AGTACT AGT^ACT
SmaI 1878 CCCGGG CCC^GGG
Tth111I 3827 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3312 TCTAGA T^CTAGA
XmaCI 1878 CCCGGG C^CCGGG
XmaI 1878 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 709 CGCG CG^CG
2911 CGCG
AflIII 2403 ACATGT A^C[GA][CT]GT
3320 ACATGT
ApaI 248 GGGCCC GGGCC^C
3855 GGGCCC
AviII 537 TGCGCA TGC^GCA
1002 TGCGCA
BalI 895 TGGCCA TGG^CCA
3335 TGGCCA
BamHI 456 GGATCC G^GATCC
1785 GGATCC
BbeI 1529 GGCGCC GGCGC^C
2999 GGCGCC
BbvCI 3646 CCTCAGC CCTCAGC(-5/-2)
1619 GCTGAGG
BglI 509 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1034 GCCCAGGAGGC
BsaAI 1946 CACGTA [CT]AC^GT[GA]
3251 TACGTG
BsaWI 1707 ACCGGT [AT]^CCGG[AT]
1788 TCCGGA
BsmI 1168 GAATGC GAATGC(1/-1)
1345 GCATTC
BspLU11I 2403 ACATGT A^CATGT
3320 ACATGT
BsrFI 1707 ACCGGT [GA]^CCGG[CT]
3298 GCCGGC
BstEII 1580 GGTGACC G^GT[ACGT]ACC
3076 GGTCACC
BstPI 1580 GGTGACC G^GT[ACGT]ACC
3076 GGTCACC
BstUI 709 CGCG CG^CG
2911 CGCG
BstXI 1498 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2692 CCAGCAAGGTGG
Cfr10I 1707 ACCGGT [GA]^CCGG[CT]
3298 GCCGGC
DrdI 725 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3567 GACTTCAGTGTC
EarI 1142 GAAGAG CTCTTC(1/4)
1181 GAAGAG
EcoO65I 1580 GGTGACC G^GT[ACGT]ACC
3076 GGTCACC
FspI 537 TGCGCA TGC^GCA
1002 TGCGCA
KasI 1529 GGCGCC G^GCGCC
2999 GGCGCC
Ksp632I 1142 GAAGAG CTCTTC(1/4)
1181 GAAGAG
MluNI 895 TGGCCA TGG^CCA
3335 TGGCCA
MscI 895 TGGCCA TGG^CCA
3335 TGGCCA
MvnI 709 CGCG CG^CG
2911 CGCG
NarI 1529 GGCGCC GG^CGCC
2999 GGCGCC
NdeI 848 CATATG CA^TATG
2363 CATATG
SacI 752 GAGCTC GAGCT^C
887 GAGCTC
SfoI 1529 GGCGCC GGC^GCC
2999 GGCGCC
XcmI 177 CCATCATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3264 CCATGCAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI