##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BbeI BbrPI BcgI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspLU11I BspMI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FseI FspI HgaI HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AocI 2594 CCTGAGG CC^T[ACGT]AGG
AvaI 637 CCCGGG C^[CT]CG[GA]G
BalI 922 TGGCCA TGG^CCA
BanII 1331 GGGCTC G[GA]GC[CT]^C
BbvCI 245 GCTGAGG CCTCAGC(-5/-2)
BciVI 1582 GTATCC GTATCC(6/5)
BsmBI 3646 CGTCTC CGTCTC(1/5)
BsoBI 637 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3775 TGTACA T^GTACA
BspHI 217 TCATGA T^CATGA
BsrBI 1378 CCGCTC CCGCTC(-3/-3)
BsrGI 3775 TGTACA T^GTACA
Bsu36I 2594 CCTGAGG CC^T[ACGT]AGG
CfoI 64 GCGC GCG^C
DrdI 3013 GACAGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 977 CCATGG C^C[GA][CT]GG
EaeI 922 TGGCCA [CT]^GGCC[GA]
Eco81I 2594 CCTGAGG CC^T[ACGT]AGG
FauI 408 GCGGG CCCGC(4/6)
HaeII 63 GGCGCT [GA]GCGC^[CT]
HapII 638 CCGG C^CGG
HhaI 64 GCGC GCG^C
HinP1I 64 GCGC G^CGC
HincII 456 GTCAAC GT[CT]^[GA]AC
HindII 456 GTCAAC GT[CT]^[GA]AC
HindIII 498 AAGCTT A^AGCTT
HpaII 638 CCGG C^CGG
MluNI 922 TGGCCA TGG^CCA
MscI 922 TGGCCA TGG^CCA
MspI 638 CCGG C^CGG
NcoI 977 CCATGG C^CATGG
PshAI 449 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 217 TCATGA T^CATGA
SmaI 637 CCCGGG CCC^GGG
SspBI 3775 TGTACA T^GTACA
TaqI 3363 TCGA T^CGA
TthHB8I 3363 TCGA T^CGA
XmaCI 637 CCCGGG C^CCGGG
XmaI 637 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 1592 GACGTC GACGT^C
2758 GACGTC
Acc65I 1063 GGTACC G^GTACC
2457 GGTACC
AcyI 1592 GACGTC G[GA]^CG[CT]C
2758 GACGTC
AseI 2507 ATTAAT AT^TAAT
3973 ATTAAT
AsnI 2507 ATTAAT AT^TAAT
3973 ATTAAT
Asp700I 393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2656 GAATGCATTC
Asp718I 1063 GGTACC G^GTACC
2457 GGTACC
AspHI 548 GAGCAC G[AT]GC[AT]^C
1548 GAGCAC
BamHI 1559 GGATCC G^GATCC
1638 GGATCC
BclI 76 TGATCA T^GATCA
2819 TGATCA
BcnI 637 CCCGG CC^[GC]GG
638 CCGGG
BlpI 1668 GCTAAGC GC^T[ACGT]AGC
2765 GCTCAGC
Bpu1102I 1668 GCTAAGC GC^T[ACGT]AGC
2765 GCTCAGC
BsaHI 1592 GACGTC G[GA]^CG[CT]C
2758 GACGTC
BsiHKAI 548 GAGCAC G[AT]GC[AT]^C
1548 GAGCAC
BstXI 310 CCATGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1769 CCAATCAGCTGG
CelII 1668 GCTAAGC GC^T[ACGT]AGC
2765 GCTCAGC
EcoNI 2461 CCTAAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2665 CCTCTGTCAGG
EcoT14I 977 CCATGG C^C[AT][AT]GG
1963 CCTTGG
EcoT22I 469 ATGCAT ATGCA^T
2658 ATGCAT
FbaI 76 TGATCA T^GATCA
2819 TGATCA
Hin1I 1592 GACGTC G[GA]^CG[CT]C
2758 GACGTC
KpnI 1063 GGTACC GGTAC^C
2457 GGTACC
MaeII 1593 ACGT A^CGT
2759 ACGT
NciI 637 CCCGG CC^[GC]GG
638 CCGGG
NdeI 3223 CATATG CA^TATG
3779 CATATG
NsiI 469 ATGCAT ATGCA^T
2658 ATGCAT
PshBI 2507 ATTAAT AT^TAAT
3973 ATTAAT
SmlI 580 CTCAAG C^T[CT][GA]AG
1713 CTCAAG
StuI 185 AGGCCT AGG^CCT
1912 AGGCCT
StyI 977 CCATGG C^C[AT][AT]GG
1963 CCTTGG
XcmI 37 CCAATCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1067 CCAGTTCTTTTGTGG
XmnI 393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2656 GAATGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanI
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrDI BsrI BssKI Bst1107I
BstF5I BstNI BstYI BstZ17I Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI SspI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII