##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII KasI
KpnI KspI MfeI MluI MroI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2432 GTAGAC GT^[AC][GT]AC
AclI 855 AACGTT AA^CGTT
AspHI 1659 GAGCAC G[AT]GC[AT]^C
AvaII 1510 GGACC G^G[AT]CC
BalI 860 TGGCCA TGG^CCA
BciVI 1783 GGATAC GTATCC(6/5)
BlpI 1937 GCTGAGC GC^T[ACGT]AGC
BmyI 1659 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 1937 GCTGAGC GC^T[ACGT]AGC
BsaWI 706 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1659 GAGCAC G[AT]GC[AT]^C
Bsp1286I 1659 GAGCAC G[AGT]GC[ACT]^C
Cac8I 299 GCTTGC GC[ACGT]^[ACGT]GC
CelII 1937 GCTGAGC GC^T[ACGT]AGC
Cfr13I 1510 GGACC G^G[ACGT]CC
EaeI 860 TGGCCA [CT]^GGCC[GA]
EcoNI 2677 CCTTCAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 405 GATATC GAT^ATC
EcoT14I 434 CCAAGG C^C[AT][AT]GG
HapII 707 CCGG C^CGG
HgaI 1255 GACGC GACGC(5/10)
HincII 1124 GTTAAC GT[CT]^[GA]AC
HindII 1124 GTTAAC GT[CT]^[GA]AC
HpaI 1124 GTTAAC GTT^AAC
HpaII 707 CCGG C^CGG
MluNI 860 TGGCCA TGG^CCA
MscI 860 TGGCCA TGG^CCA
MspI 707 CCGG C^CGG
NspI 808 GCATGT [GA]CATG^[CT]
Psp1406I 855 AACGTT AA^CGTT
PvuII 2554 CAGCTG CAG^CTG
Sau96I 1510 GGACC G^G[ACGT]CC
StyI 434 CCAAGG C^C[AT][AT]GG
XbaI 1399 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 1907 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2455 CAGAGACTG
Asp700I 888 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1880 GAATATTTTC
BbsI 1395 GTCTTC GAAGAC(2/6)
1891 GTCTTC
BpuAI 1395 GTCTTC GAAGAC(2/6)
1891 GTCTTC
BsgI 2236 CTGCAC GTGCAG(16/14)
2463 GTGCAG
BsmBI 416 CGTCTC CGTCTC(1/5)
1253 GAGACG
BsmFI 1026 GGGAC GGGAC(10/14)
2643 GGGAC
Bsp1407I 461 TGTACA T^GTACA
3043 TGTACA
BsrDI 1189 CATTGC GCAATG(2/0)
1361 CATTGC
BsrGI 461 TGTACA T^GTACA
3043 TGTACA
BstAPI 1906 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2351 GCAAGAATTGC
HaeIII 861 GGCC GG^CC
2126 GGCC
MaeII 856 ACGT A^CGT
1846 ACGT
MspA1I 1293 CCGCTG C[AC]G^C[GT]G
2554 CAGCTG
NlaIV 486 GGCTCC GG[ACGT]^[ACGT]CC
1510 GGACCC
PleI 424 GAGTC GAGTC(4/5)
1602 GAGTC
SexAI 691 ACCTGGT A^CC[AT]GGT
1354 ACCTGGT
SmlI 246 CTCAAG C^T[CT][GA]AG
2847 CTTGAG
SspBI 461 TGTACA T^GTACA
3043 TGTACA
Tsp45I 1060 GTCAC ^GT[GC]AC
2277 GTGAC
XmnI 888 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1880 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI BbvI BclI
BfaI BpmI BsaBI BsaJI BsiYI
BslI BsmAI BsmI BsrI BssKI
BstF5I BstNI BstYI DdeI DpnII
DraI EarI EcoRII FbaI Fnu4HI
FokI HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII PflMI PshBI PstI
RsaI Sau3AI ScaI ScrFI SfaNI
SfcI SspI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I
XhoII