##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AocI ApaI
AscI Asp718I AspI AvaI AviII
BamHI BbrPI BcgI BciVI BcnI
BglI BlpI BpmI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraIII
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I FseI FspI KpnI KspI
MamI MroI NaeI NciI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 599 GTATAC GT^[AC][GT]AC
AccII 1784 CGCG CG^CG
AclI 1636 AACGTT AA^CGTT
AcyI 4204 GGCGCC G[GA]^CG[CT]C
AgeI 2959 ACCGGT A^CCGGT
AhdI 2958 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2770 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4160 AGCGCT AGC^GCT
AspEI 2958 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 50 CCTAGG C^CTAGG
BanI 4204 GGCGCC G^G[CT][GA]CC
BbeI 4204 GGCGCC GGCGC^C
BbvCI 3508 GCTGAGG CCTCAGC(-5/-2)
BclI 723 TGATCA T^GATCA
BlnI 50 CCTAGG C^CTAGG
BsaAI 1090 CACGTA [CT]AC^GT[GA]
BsaHI 4204 GGCGCC G[GA]^CG[CT]C
BsaI 2965 GGTCTC GGTCTC(1/5)
BsaWI 2959 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3441 TGTACA T^GTACA
BspLU11I 286 ACATGT A^CATGT
BsrDI 2540 GCAATG GCAATG(2/0)
BsrFI 2959 ACCGGT [GA]^CCGG[CT]
BsrGI 3441 TGTACA T^GTACA
Bst1107I 599 GTATAC GTA^TAC
BstUI 1784 CGCG CG^CG
BstZ17I 599 GTATAC GTA^TAC
Cfr10I 2959 ACCGGT [GA]^CCGG[CT]
DraII 2658 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 2651 GACTAGTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3066 CCATGG C^C[GA][CT]GG
Eam1105I 2958 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4160 AGCGCT AGC^GCT
EcoO109I 2658 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 4122 GAATTC G^AATTC
EcoRV 2232 GATATC GAT^ATC
EcoT22I 3924 ATGCAT ATGCA^T
FauI 3859 GCGGG CCCGC(4/6)
FbaI 723 TGATCA T^GATCA
HgaI 1782 GACGC GACGC(5/10)
Hin1I 4204 GGCGCC G[GA]^CG[CT]C
KasI 4204 GGCGCC G^GCGCC
MfeI 2434 CAATTG C^AATTG
MluI 1783 ACGCGT A^CGCGT
MunI 2434 CAATTG C^AATTG
MvnI 1784 CGCG CG^CG
NarI 4204 GGCGCC GG^CGCC
NcoI 3066 CCATGG C^CATGG
NdeI 727 CATATG CA^TATG
NsiI 3924 ATGCAT ATGCA^T
PacI 1653 TTAATTAA TTAAT^TAA
PinAI 2959 ACCGGT A^CCGGT
PpuMI 2658 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1636 AACGTT AA^CGTT
SacI 30 GAGCTC GAGCT^C
SapI 4145 GAAGAGC GCTCTTC(1/4)
SfoI 4204 GGCGCC GGC^GCC
SspBI 3441 TGTACA T^GTACA
StuI 53 AGGCCT AGG^CCT
XbaI 3767 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 1017 CTTAAG C^TTAAG
3814 CTTAAG
Alw44I 190 GTGCAC G^TGCAC
3172 GTGCAC
ApaLI 190 GTGCAC G^TGCAC
3172 GTGCAC
Asp700I 1857 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3655 GAAGATCTTC
BfrI 1017 CTTAAG C^TTAAG
3814 CTTAAG
BsmI 313 GAATGC GAATGC(1/-1)
677 GCATTC
CfoI 4161 GCGC GCG^C
4205 GCGC
HaeII 4160 AGCGCT [GA]GCGC^[CT]
4204 GGCGCC
HapII 2731 CCGG C^CGG
2960 CCGG
HhaI 4161 GCGC GCG^C
4205 GCGC
HinP1I 4161 GCGC G^CGC
4205 GCGC
HincII 3910 GTTAAC GT[CT]^[GA]AC
3979 GTTAAC
HindII 3910 GTTAAC GT[CT]^[GA]AC
3979 GTTAAC
HindIII 859 AAGCTT A^AGCTT
1039 AAGCTT
HpaI 3910 GTTAAC GTT^AAC
3979 GTTAAC
HpaII 2731 CCGG C^CGG
2960 CCGG
MspI 2731 CCGG C^CGG
2960 CCGG
SpeI 194 ACTAGT A^CTAGT
2652 ACTAGT
SphI 323 GCATGC GCATG^C
424 GCATGC
SwaI 1064 ATTTAAAT ATTT^AAAT
1837 ATTTAAAT
XcmI 884 CCAGGTACTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3144 CCACCTGTATAGTGG
XmnI 1857 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3655 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BalI BanII BbsI BbvI
BfaI BglII BmyI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII