##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BfrI
BlnI BsaBI BsiEI BsiWI BspDI
BsrBI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV FseI FspI HpaI KasI
KpnI KspI MamI MluI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 377 TCCGGA T^CCGGA
AcyI 491 GGCGTC G[GA]^CG[CT]C
Alw44I 207 GTGCAC G^TGCAC
AocI 424 CCTAAGG CC^T[ACGT]AGG
Aor51HI 390 AGCGCT AGC^GCT
ApaI 681 GGGCCC GGGCC^C
ApaLI 207 GTGCAC G^TGCAC
AseI 4087 ATTAAT AT^TAAT
AsnI 4087 ATTAAT AT^TAAT
Asp700I 817 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1823 GCTGAGG CCTCAGC(-5/-2)
BcgI 1462 CGACTATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 358 GTATCC GTATCC(6/5)
BclI 4096 TGATCA T^GATCA
BglI 2439 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 217 AGATCT A^GATCT
BlpI 1343 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1343 GCTGAGC GC^T[ACGT]AGC
BsaAI 1121 TACGTG [CT]AC^GT[GA]
BsaHI 491 GGCGTC G[GA]^CG[CT]C
BsaI 3320 GAGACC GGTCTC(1/5)
BsaWI 377 TCCGGA [AT]^CCGG[AT]
BseAI 377 TCCGGA T^CCGGA
BsmBI 834 CGTCTC CGTCTC(1/5)
BsmI 3579 GCATTC GAATGC(1/-1)
Bsp1407I 3988 TGTACA T^GTACA
BspEI 377 TCCGGA T^CCGGA
BsrGI 3988 TGTACA T^GTACA
BssHII 610 GCGCGC G^CGCGC
BssSI 1060 CACGAG CACGAG(-5/-1)
BstAPI 796 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3899 CCATTAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 424 CCTAAGG CC^T[ACGT]AGG
CelII 1343 GCTGAGC GC^T[ACGT]AGC
DraI 4021 TTTAAA TTT^AAA
Eco47III 390 AGCGCT AGC^GCT
Eco81I 424 CCTAAGG CC^T[ACGT]AGG
FbaI 4096 TGATCA T^GATCA
HaeII 390 AGCGCT [GA]GCGC^[CT]
Hin1I 491 GGCGTC G[GA]^CG[CT]C
HindIII 1679 AAGCTT A^AGCTT
MaeII 1122 ACGT A^CGT
MroI 377 TCCGGA T^CCGGA
NcoI 2802 CCATGG C^CATGG
PaeR7I 240 CTCGAG C^TCGAG
PflMI 2349 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 4087 ATTAAT AT^TAAT
ScaI 2407 AGTACT AGT^ACT
SgrAI 844 CACCGGCG C[GA]^CCGG[CT]G
SmaI 678 CCCGGG CCC^GGG
SphI 1944 GCATGC GCATG^C
SspBI 3988 TGTACA T^GTACA
Van91I 2349 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2050 TCTAGA T^CTAGA
XcmI 591 CCAGCCTTCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 240 CTCGAG C^TCGAG
XmaCI 678 CCCGGG C^CCGGG
XmaI 678 CCCGGG C^CCGGG
XmnI 817 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 1553 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2388 GACATGGTC
AvaI 240 CTCGAG C^[CT]CG[GA]G
678 CCCGGG
BamHI 2217 GGATCC G^GATCC
2479 GGATCC
BbsI 3413 GAAGAC GAAGAC(2/6)
628 GTCTTC
BpuAI 3413 GAAGAC GAAGAC(2/6)
628 GTCTTC
BsgI 1431 CTGCAC GTGCAG(16/14)
645 GTGCAG
BsoBI 240 CTCGAG C^[CT]CG[GA]G
678 CCCGGG
BsrFI 845 ACCGGC [GA]^CCGG[CT]
1268 ACCGGC
Cfr10I 845 ACCGGC [GA]^CCGG[CT]
1268 ACCGGC
DsaI 556 CCACGG C^C[GA][CT]GG
2802 CCATGG
EcoNI 178 CCTCGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
871 CCTGCAACAGG
EcoRI 1319 GAATTC G^AATTC
1707 GAATTC
EcoT14I 2802 CCATGG C^C[AT][AT]GG
2851 CCTTGG
HgaI 492 GCGTC GACGC(5/10)
626 GCGTC
HincII 2377 GTCAAC GT[CT]^[GA]AC
2490 GTCAAC
HindII 2377 GTCAAC GT[CT]^[GA]AC
2490 GTCAAC
HphI 1238 GGTGA GGTGA(8/7)
2855 GGTGA
MfeI 136 CAATTG C^AATTG
2520 CAATTG
MslI 344 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2384 CACAGACATG
MunI 136 CAATTG C^AATTG
2520 CAATTG
PflFI 1553 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2388 GACATGGTC
PvuII 363 CAGCTG CAG^CTG
1821 CAGCTG
SacI 1199 GAGCTC GAGCT^C
1829 GAGCTC
StyI 2802 CCATGG C^C[AT][AT]GG
2851 CCTTGG
Tth111I 1553 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2388 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII