##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2667 GGTACC G^GTACC
AflIII 2376 ACATGT A^C[GA][CT]GT
Aor51HI 1459 AGCGCT AGC^GCT
ApaI 2060 GGGCCC GGGCC^C
Asp700I 2752 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2667 GGTACC G^GTACC
BamHI 789 GGATCC G^GATCC
BcnI 1352 CCCGG CC^[GC]GG
BsaAI 2336 CACGTA [CT]AC^GT[GA]
BsaBI 598 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2779 GGTCTC GGTCTC(1/5)
BsmBI 1156 CGTCTC CGTCTC(1/5)
BspHI 840 TCATGA T^CATGA
BspLU11I 2376 ACATGT A^CATGT
BsrBI 2076 GAGCGG CCGCTC(-3/-3)
BsrDI 749 GCAATG GCAATG(2/0)
BssSI 2330 CACGAG CACGAG(-5/-1)
BstAPI 1538 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 616 TTCGAA TT^CGAA
BstEII 1276 GGTGACC G^GT[ACGT]ACC
BstPI 1276 GGTGACC G^GT[ACGT]ACC
BstXI 976 CCAGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2175 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1459 AGCGCT AGC^GCT
EcoO65I 1276 GGTGACC G^GT[ACGT]ACC
EcoT14I 1272 CCTTGG C^C[AT][AT]GG
HapII 1353 CCGG C^CGG
HindIII 350 AAGCTT A^AGCTT
HpaII 1353 CCGG C^CGG
KpnI 2667 GGTACC GGTAC^C
MamI 598 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1353 CCGG C^CGG
NciI 1352 CCCGG CC^[GC]GG
NspV 616 TTCGAA TT^CGAA
PaeR7I 1072 CTCGAG C^TCGAG
PflMI 37 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2144 CAGCTG CAG^CTG
RcaI 840 TCATGA T^CATGA
SfuI 616 TTCGAA TT^CGAA
SphI 1674 GCATGC GCATG^C
StyI 1272 CCTTGG C^C[AT][AT]GG
Van91I 37 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1072 CTCGAG C^TCGAG
XmnI 2752 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1947 GTCTAC GT^[AC][GT]AC
1970 GTCTAC
AcyI 1178 GGCGTC G[GA]^CG[CT]C
2017 GACGCC
BalI 1789 TGGCCA TGG^CCA
2837 TGGCCA
BciVI 441 GGATAC GTATCC(6/5)
2818 GTATCC
BglI 1791 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2601 GCCCAGTGGGC
BsaHI 1178 GGCGTC G[GA]^CG[CT]C
2017 GACGCC
BseRI 270 CTCCTC GAGGAG(10/8)
1069 CTCCTC
BspMI 2239 ACCTGC ACCTGC(4/8)
1898 GCAGGT
BstYI 459 AGATCC [GA]^GATC[CT]
789 GGATCC
DsaI 1140 CCACGG C^C[GA][CT]GG
1525 CCGTGG
EarI 1111 CTCTTC CTCTTC(1/4)
2281 GAAGAG
FauI 1235 CCCGC CCCGC(4/6)
1550 CCCGC
HaeII 1106 AGCGCC [GA]GCGC^[CT]
1459 AGCGCT
HgaI 2017 GACGC GACGC(5/10)
1179 GCGTC
Hin1I 1178 GGCGTC G[GA]^CG[CT]C
2017 GACGCC
Ksp632I 1111 CTCTTC CTCTTC(1/4)
2281 GAAGAG
MaeII 1079 ACGT A^CGT
2337 ACGT
MflI 459 AGATCC [GA]^GATC[CT]
789 GGATCC
MluNI 1789 TGGCCA TGG^CCA
2837 TGGCCA
MscI 1789 TGGCCA TGG^CCA
2837 TGGCCA
MseI 509 TTAA T^TAA
626 TTAA
MspA1I 2144 CAGCTG C[AC]G^C[GT]G
2813 CAGCGG
PshAI 1150 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2375 GACATGTGTC
SacI 925 GAGCTC GAGCT^C
1394 GAGCTC
Tru9I 509 TTAA T^TAA
626 TTAA
XcmI 1771 CCAGCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2055 CCACTGGGCCCCTGG
XhoII 459 AGATCC [GA]^GATC[CT]
789 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoNI EcoO109I EcoRI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MslI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I