##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AvaI AvrII BbeI BbrPI
BcgI BclI BcnI BglI BlnI
BsaHI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BstBI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FbaI FseI HaeII
HapII HgaI Hin1I HpaII KasI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PstI
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 768 GGTACC G^GTACC
AclI 3099 AACGTT AA^CGTT
Asp718I 768 GGTACC G^GTACC
AspI 517 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 11 TGCGCA TGC^GCA
BamHI 1999 GGATCC G^GATCC
BanI 768 GGTACC G^G[CT][GA]CC
BbvCI 2039 CCTCAGC CCTCAGC(-5/-2)
BglII 1268 AGATCT A^GATCT
BlpI 2779 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2779 GCTGAGC GC^T[ACGT]AGC
BsaAI 1083 TACGTG [CT]AC^GT[GA]
BsaBI 1263 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1036 GAGACC GGTCTC(1/5)
BseRI 1105 GAGGAG GAGGAG(10/8)
BsmBI 1124 GAGACG CGTCTC(1/5)
BsmI 696 GCATTC GAATGC(1/-1)
BspHI 1397 TCATGA T^CATGA
BssSI 3009 CACGAG CACGAG(-5/-1)
Bst1107I 444 GTATAC GTA^TAC
BstEII 719 GGTCACC G^GT[ACGT]ACC
BstPI 719 GGTCACC G^GT[ACGT]ACC
BstXI 977 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 444 GTATAC GTA^TAC
CelII 2779 GCTGAGC GC^T[ACGT]AGC
CfoI 12 GCGC GCG^C
DraII 1478 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 3370 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 835 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1478 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 719 GGTCACC G^GT[ACGT]ACC
EcoRV 3816 GATATC GAT^ATC
FauI 29 GCGGG CCCGC(4/6)
FspI 11 TGCGCA TGC^GCA
HhaI 12 GCGC GCG^C
HinP1I 12 GCGC G^CGC
HpaI 3354 GTTAAC GTT^AAC
KpnI 768 GGTACC GGTAC^C
KspI 27 CCGCGG CCGC^GG
MamI 1263 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 776 CCATGG C^CATGG
PflFI 517 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3998 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1478 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3768 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3099 AACGTT AA^CGTT
RcaI 1397 TCATGA T^CATGA
SacI 907 GAGCTC GAGCT^C
SacII 27 CCGCGG CCGC^GG
SpeI 2686 ACTAGT A^CTAGT
TaqI 1836 TCGA T^CGA
Tth111I 517 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1836 TCGA T^CGA
Van91I 3998 CCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1120 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 28 CGCG CG^CG
534 CGCG
AflII 2849 CTTAAG C^TTAAG
3797 CTTAAG
AocI 835 CCTAAGG CC^T[ACGT]AGG
3896 CCTTAGG
AseI 2145 ATTAAT AT^TAAT
2199 ATTAAT
AsnI 2145 ATTAAT AT^TAAT
2199 ATTAAT
AspHI 907 GAGCTC G[AT]GC[AT]^C
1311 GAGCAC
BalI 2712 TGGCCA TGG^CCA
3601 TGGCCA
BanII 907 GAGCTC G[GA]GC[CT]^C
1108 GAGCCC
BbsI 202 GAAGAC GAAGAC(2/6)
4074 GTCTTC
BfrI 2849 CTTAAG C^TTAAG
3797 CTTAAG
BpuAI 202 GAAGAC GAAGAC(2/6)
4074 GTCTTC
BsgI 2565 GTGCAG GTGCAG(16/14)
3445 GTGCAG
BsiHKAI 907 GAGCTC G[AT]GC[AT]^C
1311 GAGCAC
Bsp1407I 1343 TGTACA T^GTACA
1411 TGTACA
BspMI 841 GCAGGT ACCTGC(4/8)
2241 GCAGGT
BsrDI 617 CATTGC GCAATG(2/0)
3363 GCAATG
BsrGI 1343 TGTACA T^GTACA
1411 TGTACA
BstUI 28 CGCG CG^CG
534 CGCG
Bsu36I 835 CCTAAGG CC^T[ACGT]AGG
3896 CCTTAGG
EaeI 2712 TGGCCA [CT]^GGCC[GA]
3601 TGGCCA
Eco81I 835 CCTAAGG CC^T[ACGT]AGG
3896 CCTTAGG
EcoT22I 1380 ATGCAT ATGCA^T
3659 ATGCAT
HincII 3354 GTTAAC GT[CT]^[GA]AC
3680 GTTGAC
HindII 3354 GTTAAC GT[CT]^[GA]AC
3680 GTTGAC
MluNI 2712 TGGCCA TGG^CCA
3601 TGGCCA
MscI 2712 TGGCCA TGG^CCA
3601 TGGCCA
MvnI 28 CGCG CG^CG
534 CGCG
NdeI 413 CATATG CA^TATG
455 CATATG
NsiI 1380 ATGCAT ATGCA^T
3659 ATGCAT
PshBI 2145 ATTAAT AT^TAAT
2199 ATTAAT
SapI 2277 GAAGAGC GCTCTTC(1/4)
1672 GCTCTTC
SmlI 2849 CTTAAG C^T[CT][GA]AG
3797 CTTAAG
SspBI 1343 TGTACA T^GTACA
1411 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AvaII BbvI BciVI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmnI