##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AhdI AscI Asp718I AspEI AvaI
AviII AvrII BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI Eam1105I
EcoO65I EcoRI EcoRV FbaI FseI
FspI KpnI KspI MluI NheI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI Psp1406I PvuI
RcaI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 804 GTCTAC GT^[AC][GT]AC
AccII 1447 CGCG CG^CG
AccIII 3418 TCCGGA T^CCGGA
AgeI 1587 ACCGGT A^CCGGT
Alw44I 3133 GTGCAC G^TGCAC
AlwNI 1226 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 955 CCTCAGG CC^T[ACGT]AGG
Aor51HI 677 AGCGCT AGC^GCT
ApaI 1522 GGGCCC GGGCC^C
ApaLI 3133 GTGCAC G^TGCAC
AseI 2298 ATTAAT AT^TAAT
AsnI 2298 ATTAAT AT^TAAT
Asp700I 3622 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1095 CCTCAGC CCTCAGC(-5/-2)
BcgI 2350 GCAAGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 335 CCGGG CC^[GC]GG
BsaBI 2443 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3123 GGTCTC GGTCTC(1/5)
BseAI 3418 TCCGGA T^CCGGA
BsiEI 574 CGGCCG CG[GA][CT]^CG
BspEI 3418 TCCGGA T^CCGGA
BsrBI 1514 GAGCGG CCGCTC(-3/-3)
BssSI 2886 CTCGTG CACGAG(-5/-1)
BstAPI 1225 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1447 CGCG CG^CG
BstXI 879 CCAGGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 955 CCTCAGG CC^T[ACGT]AGG
CpoI 1584 CGGACCG CG^G[AT]CCG
DraIII 1750 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1582 GACGGACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 574 CGGCCG C^GGCCG
EclXI 574 CGGCCG C^GGCCG
Eco47III 677 AGCGCT AGC^GCT
Eco52I 574 CGGCCG C^GGCCG
Eco81I 955 CCTCAGG CC^T[ACGT]AGG
HgaI 2801 GCGTC GACGC(5/10)
HpaI 3236 GTTAAC GTT^AAC
MamI 2443 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3418 TCCGGA T^CCGGA
MvnI 1447 CGCG CG^CG
NaeI 1090 GCCGGC GCC^GGC
NciI 335 CCGGG CC^[GC]GG
NgoMIV 1090 GCCGGC G^CCGGC
PflFI 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1587 ACCGGT A^CCGGT
PpuMI 1707 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2298 ATTAAT AT^TAAT
RsrII 1584 CGGACCG CG^G[AT]CCG
SacI 3266 GAGCTC GAGCT^C
SapI 3327 GAAGAGC GCTCTTC(1/4)
SmlI 2541 CTTGAG C^T[CT][GA]AG
SspI 3813 AATATT AAT^ATT
StuI 1691 AGGCCT AGG^CCT
TaqI 1200 TCGA T^CGA
Tth111I 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1200 TCGA T^CGA
XbaI 1726 TCTAGA T^CTAGA
XmnI 3622 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 496 GGCGCC G[GA]^CG[CT]C
1423 GGCGCC
BamHI 547 GGATCC G^GATCC
3320 GGATCC
BbeI 496 GGCGCC GGCGC^C
1423 GGCGCC
BbsI 2598 GAAGAC GAAGAC(2/6)
3437 GAAGAC
BglI 683 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1210 GCCCCTCTGGC
BpuAI 2598 GAAGAC GAAGAC(2/6)
3437 GAAGAC
BsaHI 496 GGCGCC G[GA]^CG[CT]C
1423 GGCGCC
BsaWI 1587 ACCGGT [AT]^CCGG[AT]
3418 TCCGGA
BsmI 1083 GAATGC GAATGC(1/-1)
2376 GAATGC
BspMI 1146 ACCTGC ACCTGC(4/8)
2828 ACCTGC
BsrFI 1090 GCCGGC [GA]^CCGG[CT]
1587 ACCGGT
Cfr10I 1090 GCCGGC [GA]^CCGG[CT]
1587 ACCGGT
EcoNI 58 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1617 CCTGGCCCAGG
Hin1I 496 GGCGCC G[GA]^CG[CT]C
1423 GGCGCC
HindIII 1896 AAGCTT A^AGCTT
1935 AAGCTT
KasI 496 GGCGCC G^GCGCC
1423 GGCGCC
NarI 496 GGCGCC GG^CGCC
1423 GGCGCC
NdeI 5 CATATG CA^TATG
1911 CATATG
PflMI 213 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1537 CCAGGAGGTGG
PshAI 834 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3117 GACCAAGGTC
PstI 300 CTGCAG CTGCA^G
1598 CTGCAG
SfoI 496 GGCGCC GGC^GCC
1423 GGCGCC
SphI 2832 GCATGC GCATG^C
3159 GCATGC
Van91I 213 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1537 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII