##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FauI
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KpnI KspI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 2082 ACATGT A^C[GA][CT]GT
Alw44I 95 GTGCAC G^TGCAC
ApaLI 95 GTGCAC G^TGCAC
Asp700I 1349 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 95 GTGCAC G[AT]GC[AT]^C
AspI 311 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2045 GGTGCC G^G[CT][GA]CC
BglII 4014 AGATCT A^GATCT
BpmI 987 CTCCAG CTGGAG(16/14)
BsgI 1221 CTGCAC GTGCAG(16/14)
BsiHKAI 95 GTGCAC G[AT]GC[AT]^C
BspLU11I 2082 ACATGT A^CATGT
BsrBI 1069 CCGCTC CCGCTC(-3/-3)
BstXI 1081 CCAAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 2818 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 1460 GCGC GCG^C
DraII 3808 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 2230 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 827 CCGTGG C^C[GA][CT]GG
EcoO109I 3808 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 3827 GATATC GAT^ATC
HaeII 1459 GGCGCT [GA]GCGC^[CT]
HhaI 1460 GCGC GCG^C
HinP1I 1460 GCGC G^CGC
HindIII 3073 AAGCTT A^AGCTT
PflFI 311 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 3514 CTGCAG CTGCA^G
SapI 1142 GAAGAGC GCTCTTC(1/4)
SwaI 2216 ATTTAAAT ATTT^AAAT
TaqI 1336 TCGA T^CGA
Tth111I 311 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1336 TCGA T^CGA
XbaI 1980 TCTAGA T^CTAGA
XcmI 1314 CCAAACTGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1349 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AciI 1069 CCGC CCGC(-3/-1)
874 GCGG
AflII 2122 CTTAAG C^TTAAG
2831 CTTAAG
AlwNI 456 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
581 CAGTTACTG
AocI 1610 CCTTAGG CC^T[ACGT]AGG
1622 CCTTAGG
AvaII 3552 GGTCC G^G[AT]CC
3624 GGTCC
BalI 830 TGGCCA TGG^CCA
2582 TGGCCA
BclI 2841 TGATCA T^GATCA
2973 TGATCA
BfrI 2122 CTTAAG C^TTAAG
2831 CTTAAG
BmyI 95 GTGCAC G[AGT]GC[ACT]^C
1310 GTGCCC
BsaBI 44 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
218 GATTTTTATC
BsmFI 1035 GTCCC GGGAC(10/14)
3625 GTCCC
Bsp1286I 95 GTGCAC G[AGT]GC[ACT]^C
1310 GTGCCC
BspHI 1576 TCATGA T^CATGA
2664 TCATGA
Bsu36I 1610 CCTTAGG CC^T[ACGT]AGG
1622 CCTTAGG
EaeI 830 TGGCCA [CT]^GGCC[GA]
2582 TGGCCA
Eco81I 1610 CCTTAGG CC^T[ACGT]AGG
1622 CCTTAGG
EcoT22I 3913 ATGCAT ATGCA^T
4038 ATGCAT
FbaI 2841 TGATCA T^GATCA
2973 TGATCA
HincII 431 GTTGAC GT[CT]^[GA]AC
976 GTCAAC
HindII 431 GTTGAC GT[CT]^[GA]AC
976 GTCAAC
MaeII 1273 ACGT A^CGT
3600 ACGT
MamI 44 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
218 GATTTTTATC
MluNI 830 TGGCCA TGG^CCA
2582 TGGCCA
MscI 830 TGGCCA TGG^CCA
2582 TGGCCA
MslI 72 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4060 CACTTAAATG
MwoI 187 GCCCTCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1071 GCTCATCTCGC
NsiI 3913 ATGCAT ATGCA^T
4038 ATGCAT
NspI 125 GCATGT [GA]CATG^[CT]
2082 ACATGT
PflMI 2478 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3555 CCACATCATGG
RcaI 1576 TCATGA T^CATGA
2664 TCATGA
ScaI 108 AGTACT AGT^ACT
2444 AGTACT
SpeI 2436 ACTAGT A^CTAGT
2933 ACTAGT
Van91I 2478 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3555 CCACATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI BbvI BfaI BsaJI
BsiYI BslI BsmAI BsmI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI NdeII NlaIII NlaIV
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII