##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII BfrI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII BstAPI BstEII BstPI
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FauI FseI FspI KspI MluI
MroI MspA1I NotI NsiI PacI
PaeR7I PinAI PshAI Psp1406I PvuI
PvuII RsrII SacII SapI SfiI
SgrAI SnaBI SphI StuI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 4916 GACGTC GACGT^C
AocI 4382 CCTCAGG CC^T[ACGT]AGG
ApaI 262 GGGCCC GGGCC^C
BamHI 1452 GGATCC G^GATCC
BbeI 203 GGCGCC GGCGC^C
BclI 5138 TGATCA T^GATCA
BglII 2006 AGATCT A^GATCT
BsaBI 4361 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 904 GAGGAG GAGGAG(10/8)
BsiEI 4175 CGACCG CG[GA][CT]^CG
BsmBI 329 GAGACG CGTCTC(1/5)
Bsp1407I 2722 TGTACA T^GTACA
BsrFI 393 GCCGGC [GA]^CCGG[CT]
BsrGI 2722 TGTACA T^GTACA
BssSI 1893 CACGAG CACGAG(-5/-1)
BstBI 1399 TTCGAA TT^CGAA
Bsu36I 4382 CCTCAGG CC^T[ACGT]AGG
Cfr10I 393 GCCGGC [GA]^CCGG[CT]
DrdI 5078 GACTCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 5282 CTCTTC CTCTTC(1/4)
Eco81I 4382 CCTCAGG CC^T[ACGT]AGG
FbaI 5138 TGATCA T^GATCA
HaeII 203 GGCGCC [GA]GCGC^[CT]
HgaI 5202 GCGTC GACGC(5/10)
HpaI 4812 GTTAAC GTT^AAC
KasI 203 GGCGCC G^GCGCC
Ksp632I 5282 CTCTTC CTCTTC(1/4)
MamI 4361 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 393 GCCGGC GCC^GGC
NarI 203 GGCGCC GG^CGCC
NcoI 4612 CCATGG C^CATGG
NdeI 1271 CATATG CA^TATG
NgoMIV 393 GCCGGC G^CCGGC
NheI 1222 GCTAGC G^CTAGC
NruI 3517 TCGCGA TCG^CGA
NspV 1399 TTCGAA TT^CGAA
SacI 4161 GAGCTC GAGCT^C
SbfI 2245 CCTGCAGG CCTGCA^GG
ScaI 5806 AGTACT AGT^ACT
SexAI 4489 ACCAGGT A^CC[AT]GGT
SfoI 203 GGCGCC GGC^GCC
SfuI 1399 TTCGAA TT^CGAA
SmaI 235 CCCGGG CCC^GGG
Sse8387I 2245 CCTGCAGG CCTGCA^GG
SspBI 2722 TGTACA T^GTACA
SwaI 2075 ATTTAAAT ATTT^AAAT
XcmI 5617 CCAGTCTGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 235 CCCGGG C^CCGGG
XmaI 235 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 4710 GGTACC G^GTACC
4833 GGTACC
AcyI 203 GGCGCC G[GA]^CG[CT]C
4916 GACGTC
Alw44I 1356 GTGCAC G^TGCAC
3038 GTGCAC
AlwNI 1012 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
4624 CAGAACCTG
ApaLI 1356 GTGCAC G^TGCAC
3038 GTGCAC
Asp718I 4710 GGTACC G^GTACC
4833 GGTACC
AspI 54 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
165 GACGGGGTC
AvrII 618 CCTAGG C^CTAGG
675 CCTAGG
BbrPI 289 CACGTG CAC^GTG
6184 CACGTG
BbvCI 1604 CCTCAGC CCTCAGC(-5/-2)
3689 CCTCAGC
BciVI 2265 GGATAC GTATCC(6/5)
4366 GTATCC
BglI 206 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3261 GCCAGCATGGC
BlnI 618 CCTAGG C^CTAGG
675 CCTAGG
BlpI 3252 GCTCAGC GC^T[ACGT]AGC
4617 GCTGAGC
Bpu1102I 3252 GCTCAGC GC^T[ACGT]AGC
4617 GCTGAGC
BsaAI 289 CACGTG [CT]AC^GT[GA]
6184 CACGTG
BsaHI 203 GGCGCC G[GA]^CG[CT]C
4916 GACGTC
BsaI 1961 GAGACC GGTCTC(1/5)
359 GGTCTC
BsgI 26 CTGCAC GTGCAG(16/14)
196 GTGCAG
BspMI 1529 GCAGGT ACCTGC(4/8)
2248 GCAGGT
BsrDI 5207 CATTGC GCAATG(2/0)
3439 GCAATG
Bst1107I 603 GTATAC GTA^TAC
2709 GTATAC
BstYI 1452 GGATCC [GA]^GATC[CT]
2006 AGATCT
BstZ17I 603 GTATAC GTA^TAC
2709 GTATAC
CelII 3252 GCTCAGC GC^T[ACGT]AGC
4617 GCTGAGC
DraIII 4488 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6184 CACGTGGTG
DsaI 3028 CCACGG C^C[GA][CT]GG
4612 CCATGG
EcoRV 4541 GATATC GAT^ATC
4578 GATATC
Hin1I 203 GGCGCC G[GA]^CG[CT]C
4916 GACGTC
KpnI 4710 GGTACC GGTAC^C
4833 GGTACC
MfeI 723 CAATTG C^AATTG
3300 CAATTG
MflI 1452 GGATCC [GA]^GATC[CT]
2006 AGATCT
MunI 723 CAATTG C^AATTG
3300 CAATTG
PflFI 54 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
165 GACGGGGTC
PmaCI 289 CACGTG CAC^GTG
6184 CACGTG
PmeI 1262 GTTTAAAC GTTT^AAAC
4567 GTTTAAAC
PmlI 289 CACGTG CAC^GTG
6184 CACGTG
PpuMI 4818 GGGACCC [GA]G^G[AT]CC[CT]
4984 GGGTCCC
SalI 4173 GTCGAC G^TCGAC
5385 GTCGAC
Tth111I 54 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
165 GACGGGGTC
XhoII 1452 GGATCC [GA]^GATC[CT]
2006 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspLU11I BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmnI