##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AflII AflIII AgeI
AocI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BfrI BlnI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco81I EcoRI EcoRV
EcoT22I FspI HindIII HpaI KpnI
MluI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AclI 3376 AACGTT AA^CGTT
AcsI 2609 AAATTT [GA]^AATT[CT]
Aor51HI 324 AGCGCT AGC^GCT
ApoI 2609 AAATTT [GA]^AATT[CT]
AspI 4688 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 141 GCAAGCCCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1 TGATCA T^GATCA
BglII 4121 AGATCT A^GATCT
BsaAI 4044 TACGTG [CT]AC^GT[GA]
BsaBI 3035 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstEII 1470 GGTCACC G^GT[ACGT]ACC
BstPI 1470 GGTCACC G^GT[ACGT]ACC
Eco47III 324 AGCGCT AGC^GCT
EcoNI 3098 CCTGCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1470 GGTCACC G^GT[ACGT]ACC
FbaI 1 TGATCA T^GATCA
FseI 746 GGCCGGCC GGCCGG^CC
MamI 3035 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2761 CAATTG C^AATTG
MunI 2761 CAATTG C^AATTG
NheI 2715 GCTAGC G^CTAGC
NspI 3860 GCATGT [GA]CATG^[CT]
PflFI 4688 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3376 AACGTT AA^CGTT
SexAI 2233 ACCAGGT A^CC[AT]GGT
SgrAI 4525 CGCCGGTG C[GA]^CCGG[CT]G
SspI 2544 AATATT AAT^ATT
Tth111I 4688 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 703 CCATCCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 944 GACGTC GACGT^C
3072 GACGTC
AccIII 1079 TCCGGA T^CCGGA
4439 TCCGGA
AhdI 401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5099 GACGCTTTGTC
Alw44I 3681 GTGCAC G^TGCAC
4710 GTGCAC
ApaLI 3681 GTGCAC G^TGCAC
4710 GTGCAC
AspEI 401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5099 GACGCTTTGTC
BbeI 2871 GGCGCC GGCGC^C
4716 GGCGCC
BbsI 1787 GAAGAC GAAGAC(2/6)
1149 GTCTTC
BciVI 2201 GGATAC GTATCC(6/5)
4064 GTATCC
BglI 1676 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2508 GCCTCCACGGC
BpuAI 1787 GAAGAC GAAGAC(2/6)
1149 GTCTTC
BseAI 1079 TCCGGA T^CCGGA
4439 TCCGGA
BsmBI 1389 GAGACG CGTCTC(1/5)
4521 GAGACG
BspEI 1079 TCCGGA T^CCGGA
4439 TCCGGA
BstYI 3700 GGATCT [GA]^GATC[CT]
4121 AGATCT
DraIII 570 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3786 CACAGGGTG
Eam1105I 401 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5099 GACGCTTTGTC
KasI 2871 GGCGCC G^GCGCC
4716 GGCGCC
KspI 736 CCGCGG CCGC^GG
4203 CCGCGG
MflI 3700 GGATCT [GA]^GATC[CT]
4121 AGATCT
MroI 1079 TCCGGA T^CCGGA
4439 TCCGGA
NarI 2871 GGCGCC GG^CGCC
4716 GGCGCC
SacI 3666 GAGCTC GAGCT^C
3719 GAGCTC
SacII 736 CCGCGG CCGC^GG
4203 CCGCGG
SfoI 2871 GGCGCC GGC^GCC
4716 GGCGCC
XhoII 3700 GGATCT [GA]^GATC[CT]
4121 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI