##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AclI AgeI Aor51HI AscI AseI
AsnI AviII BbeI BglII BlpI
Bpu1102I BspDI BssHII BstBI CelII
ClaI Eco47III EcoRI EcoRV EcoT22I
FseI FspI HindIII KasI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 400 GACGTC GACGT^C
AflII 2104 CTTAAG C^TTAAG
Alw44I 545 GTGCAC G^TGCAC
AocI 5296 CCTCAGG CC^T[ACGT]AGG
ApaLI 545 GTGCAC G^TGCAC
Asp700I 3315 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1965 CACGTG CAC^GTG
BbsI 2262 GTCTTC GAAGAC(2/6)
BciVI 1633 GGATAC GTATCC(6/5)
BfrI 2104 CTTAAG C^TTAAG
BpuAI 2262 GTCTTC GAAGAC(2/6)
BsaBI 2059 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 54 CGGCCG CG[GA][CT]^CG
BsiWI 2008 CGTACG C^GTACG
BsmI 3618 GCATTC GAATGC(1/-1)
BspHI 5127 TCATGA T^CATGA
Bst1107I 5348 GTATAC GTA^TAC
BstEII 1669 GGTCACC G^GT[ACGT]ACC
BstPI 1669 GGTCACC G^GT[ACGT]ACC
BstZ17I 5348 GTATAC GTA^TAC
Bsu36I 5296 CCTCAGG CC^T[ACGT]AGG
CpoI 2243 CGGACCG CG^G[AT]CCG
DraIII 1826 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 54 CGGCCG C^GGCCG
EclXI 54 CGGCCG C^GGCCG
Eco52I 54 CGGCCG C^GGCCG
Eco81I 5296 CCTCAGG CC^T[ACGT]AGG
EcoNI 1042 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1669 GGTCACC G^GT[ACGT]ACC
HincII 2449 GTTAAC GT[CT]^[GA]AC
HindII 2449 GTTAAC GT[CT]^[GA]AC
HpaI 2449 GTTAAC GTT^AAC
KspI 51 CCGCGG CCGC^GG
MamI 2059 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1194 GCCGGC GCC^GGC
NcoI 129 CCATGG C^CATGG
NgoMIV 1194 GCCGGC G^CCGGC
PmaCI 1965 CACGTG CAC^GTG
PmlI 1965 CACGTG CAC^GTG
PshAI 3703 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5127 TCATGA T^CATGA
RsrII 2243 CGGACCG CG^G[AT]CCG
SacII 51 CCGCGG CCGC^GG
SfiI 4025 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 5387 AGGCCT AGG^CCT
XbaI 559 TCTAGA T^CTAGA
XmnI 3315 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1097 GGTACC G^GTACC
1281 GGTACC
AccI 1415 GTAGAC GT^[AC][GT]AC
5348 GTATAC
AccIII 633 TCCGGA T^CCGGA
2266 TCCGGA
AcyI 400 GACGTC G[GA]^CG[CT]C
1343 GACGCC
AhdI 1992 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2270 GACCCTCAGTC
AlwNI 2283 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
4871 CAGCCTCTG
Asp718I 1097 GGTACC G^GTACC
1281 GGTACC
AspEI 1992 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2270 GACCCTCAGTC
BsaHI 400 GACGTC G[GA]^CG[CT]C
1343 GACGCC
BseAI 633 TCCGGA T^CCGGA
2266 TCCGGA
Bsp1407I 2048 TGTACA T^GTACA
5533 TGTACA
BspEI 633 TCCGGA T^CCGGA
2266 TCCGGA
BsrGI 2048 TGTACA T^GTACA
5533 TGTACA
BssSI 2304 CTCGTG CACGAG(-5/-1)
2359 CTCGTG
DraI 3032 TTTAAA TTT^AAA
4530 TTTAAA
Eam1105I 1992 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2270 GACCCTCAGTC
EarI 1145 GAAGAG CTCTTC(1/4)
1759 GAAGAG
Hin1I 400 GACGTC G[GA]^CG[CT]C
1343 GACGCC
KpnI 1097 GGTACC GGTAC^C
1281 GGTACC
Ksp632I 1145 GAAGAG CTCTTC(1/4)
1759 GAAGAG
MroI 633 TCCGGA T^CCGGA
2266 TCCGGA
PflMI 1585 CCATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2584 CCACCTCCTGG
PpuMI 2144 GGGACCT [GA]G^G[AT]CC[CT]
2284 AGGTCCT
SexAI 1284 ACCAGGT A^CC[AT]GGT
2281 ACCAGGT
SspBI 2048 TGTACA T^GTACA
5533 TGTACA
SspI 3555 AATATT AAT^ATT
5546 AATATT
Van91I 1585 CCATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2584 CCACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AspHI
AspI AvaI AvaII AvrII BalI
BamHI BanI BanII BbvCI BbvI
BcgI BclI BcnI BfaI BglI
BlnI BmyI BpmI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoI XhoII
XmaCI XmaI