##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
Acc65I AccIII AclI AscI Asp718I
AviII BcgI BseAI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
EagI EclXI Eco52I EcoO65I FseI
FspI HindIII KpnI KspI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PmeI Psp1406I SacII SalI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AgeI 1875 ACCGGT A^CCGGT
AhdI 2558 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5105 GTGCAC G^TGCAC
ApaLI 5105 GTGCAC G^TGCAC
AspEI 2558 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5716 GGATCC G^GATCC
BbeI 1059 GGCGCC GGCGC^C
BsiEI 4915 CGATCG CG[GA][CT]^CG
BspLU11I 8081 ACATGT A^CATGT
BspMI 9703 ACCTGC ACCTGC(4/8)
CpoI 6561 CGGACCG CG^G[AT]CCG
DraIII 8138 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 6388 GACTGGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2558 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HpaI 2738 GTTAAC GTT^AAC
KasI 1059 GGCGCC G^GCGCC
NaeI 4340 GCCGGC GCC^GGC
NarI 1059 GGCGCC GG^CGCC
NgoMIV 4340 GCCGGC G^CCGGC
PinAI 1875 ACCGGT A^CCGGT
PshAI 689 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4915 CGATCG CGAT^CG
RsrII 6561 CGGACCG CG^G[AT]CCG
SapI 4987 GAAGAGC GCTCTTC(1/4)
SfoI 1059 GGCGCC GGC^GCC
SmaI 6198 CCCGGG CCC^GGG
SspI 8148 AATATT AAT^ATT
XmaCI 6198 CCCGGG C^CCGGG
XmaI 6198 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 407 GACGTC GACGT^C
5039 GACGTC
AccI 1973 GTAGAC GT^[AC][GT]AC
3707 GTCTAC
AocI 6629 CCTGAGG CC^T[ACGT]AGG
8541 CCTAAGG
AseI 3416 ATTAAT AT^TAAT
9843 ATTAAT
AsnI 3416 ATTAAT AT^TAAT
9843 ATTAAT
AvrII 5398 CCTAGG C^CTAGG
7655 CCTAGG
BbrPI 866 CACGTG CAC^GTG
5108 CACGTG
BlnI 5398 CCTAGG C^CTAGG
7655 CCTAGG
Bsp1407I 1029 TGTACA T^GTACA
6956 TGTACA
BsrBI 3567 CCGCTC CCGCTC(-3/-3)
3514 GAGCGG
BsrFI 1875 ACCGGT [GA]^CCGG[CT]
4340 GCCGGC
BsrGI 1029 TGTACA T^GTACA
6956 TGTACA
BstAPI 1694 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3404 GCAGTCATTGC
Bsu36I 6629 CCTGAGG CC^T[ACGT]AGG
8541 CCTAAGG
Cfr10I 1875 ACCGGT [GA]^CCGG[CT]
4340 GCCGGC
Eco81I 6629 CCTGAGG CC^T[ACGT]AGG
8541 CCTAAGG
EcoRI 2660 GAATTC G^AATTC
5306 GAATTC
EcoRV 779 GATATC GAT^ATC
1820 GATATC
PmaCI 866 CACGTG CAC^GTG
5108 CACGTG
PmlI 866 CACGTG CAC^GTG
5108 CACGTG
PshBI 3416 ATTAAT AT^TAAT
9843 ATTAAT
SexAI 1444 ACCAGGT A^CC[AT]GGT
6246 ACCTGGT
SspBI 1029 TGTACA T^GTACA
6956 TGTACA
StuI 940 AGGCCT AGG^CCT
9577 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco47III EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SpeI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoII XmnI